Phospho-BCKDHA (Ser292/Ser337) Antibody

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AF3826 at 1/100 staining Mouse heart tissue by IHC-P.

Product:	Phospho-BCKDHA (Ser292/Ser337) Antibody
Catalog:	AF3826
Description:	Rabbit polyclonal antibody to Phospho-BCKDHA (Ser292/Ser337)
Application:	IHC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	50kD(Calculated).
Uniprot:	P12694
RRID:	AB_2847140

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Phospho-BCKDHA (Ser292/Ser337) Antibody detects endogenous levels of BCKDHA only when phosphorylated at Ser292/337.

RRID:

AB_2847140
Cite Format: Affinity Biosciences Cat# AF3826, RRID:AB_2847140.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

;Branched chain alpha keto acid dehydrogenase E1 component alpha polypeptide; FLJ45695; OVD1A; 2 oxoisovalerate dehydrogenase (lipoamide); 2 oxoisovalerate dehydrogenase subunit alpha, mitochondrial; 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial; BCKDE1A; BCKDH E1 alpha; BCKDH E1-alpha; BCKDHA; Branched chain alpha keto acid dehydrogenase E1 component alpha chain; Branched chain keto acid dehydrogenase E1 alpha polypeptide; Branched chain keto acid dehydrogenase E1, alpha polypeptide (maple syrup urine disease); Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; MSU; MSUD1; ODBA_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human BCKDHA around the phosphorylation site of Ser292/337.

Uniprot:

P12694(ODBA_HUMAN) >>Visit HPA database.

Gene(ID):

BCKDHA(593)

Sequence:

P12694 ODBA_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAVAIAAARVWRLNRGLSQAALLLLRQPGARGLARSHPPRQQQQFSSLDDKPQFPGASAEFIDKLEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPKEKVLKLYKSMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALDNTDLVFGQYREAGVLMYRDYPLELFMAQCYGNISDLGKGRQMPVHYGCKERHFVTISSPLATQIPQAVGAAYAAKRANANRVVICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYRGDGIAARGPGYGIMSIRVDGNDVFAVYNATKEARRRAVAENQPFLIEAMTYRIGHHSTSDDSSAYRSVDEVNYWDKQDHPISRLRHYLLSQGWWDEEQEKAWRKQSRRKVMEAFEQAERKPKPNPNLLFSDVYQEMPAQLRKQQESLARHLQTYGEHYPLDHFDK

PTMs - P12694 As Substrate

P12694

Site	PTM Type	Enzyme	Source
S46	Phosphorylation		Uniprot
S47	Phosphorylation		Uniprot
Y106	Phosphorylation		Uniprot
K107	Ubiquitination		Uniprot
T114	Phosphorylation		Uniprot
R117	Methylation		Uniprot
S206	Phosphorylation		Uniprot
S207	Phosphorylation		Uniprot
K224	Ubiquitination		Uniprot
S295	Phosphorylation		Uniprot
Y307	Phosphorylation		Uniprot
K311	Ubiquitination		Uniprot
S337	Phosphorylation	O14874 (BCKDK)	Uniprot
T338	Phosphorylation		Uniprot
S339	Phosphorylation		Uniprot
S342	Phosphorylation		Uniprot
S343	Phosphorylation		Uniprot
Y345	Phosphorylation		Uniprot
S347	Phosphorylation		Uniprot
Y353	Phosphorylation		Uniprot
K356	Ubiquitination		Uniprot
K445	Acetylation		Uniprot

Research Backgrounds

P12694_ODBA_HUMAN

Function:

The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).

Subcellular Location:

Mitochondrion matrix.

Subunit Structure:

Heterotetramer of alpha and beta chains.

Family&Domains:

Belongs to the BCKDHA family.

Research Fields

· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.

· Metabolism > Carbohydrate metabolism > Propanoate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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Phospho-BCKDHA (Ser292/Ser337) Antibody - #AF3826