Product: Phospho-53BP1 (Thr543) Antibody
Catalog: AF3817
Description: Rabbit polyclonal antibody to Phospho-53BP1 (Thr543)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 214kD(Calculated).
Uniprot: Q12888
RRID: AB_2847131

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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-53BP1 (Thr543) Antibody detects endogenous levels of 53BP1 only when phosphorylated at Thr543.
RRID:
AB_2847131
Cite Format: Affinity Biosciences Cat# AF3817, RRID:AB_2847131.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

53 BP1; 53BP1; FLJ41424; MGC138366; p202; p53 binding protein 1; p53 BP1; p53-binding protein 1; p53BP1; TP53 BP1; TP53B_HUMAN; Tp53bp1; TRP53 BP1; Tumor protein 53 binding protein 1; Tumor protein p53 binding protein 1; Tumor suppressor p53 binding protein 1; Tumor suppressor p53-binding protein 1;

Immunogens

Immunogen:

A synthesized peptide derived from human 53BP1 around the phosphorylation site of Thr543.

Uniprot:
Gene(ID):
Sequence:
MDPTGSQLDSDFSQQDTPCLIIEDSQPESQVLEDDSGSHFSMLSRHLPNLQTHKENPVLDVVSNPEQTAGEERGDGNSGFNEHLKENKVADPVDSSNLDTCGSISQVIEQLPQPNRTSSVLGMSVESAPAVEEEKGEELEQKEKEKEEDTSGNTTHSLGAEDTASSQLGFGVLELSQSQDVEENTVPYEVDKEQLQSVTTNSGYTRLSDVDANTAIKHEEQSNEDIPIAEQSSKDIPVTAQPSKDVHVVKEQNPPPARSEDMPFSPKASVAAMEAKEQLSAQELMESGLQIQKSPEPEVLSTQEDLFDQSNKTVSSDGCSTPSREEGGCSLASTPATTLHLLQLSGQRSLVQDSLSTNSSDLVAPSPDAFRSTPFIVPSSPTEQEGRQDKPMDTSVLSEEGGEPFQKKLQSGEPVELENPPLLPESTVSPQASTPISQSTPVFPPGSLPIPSQPQFSHDIFIPSPSLEEQSNDGKKDGDMHSSSLTVECSKTSEIEPKNSPEDLGLSLTGDSCKLMLSTSEYSQSPKMESLSSHRIDEDGENTQIEDTEPMSPVLNSKFVPAENDSILMNPAQDGEVQLSQNDDKTKGDDTDTRDDISILATGCKGREETVAEDVCIDLTCDSGSQAVPSPATRSEALSSVLDQEEAMEIKEHHPEEGSSGSEVEEIPETPCESQGEELKEENMESVPLHLSLTETQSQGLCLQKEMPKKECSEAMEVETSVISIDSPQKLAILDQELEHKEQEAWEEATSEDSSVVIVDVKEPSPRVDVSCEPLEGVEKCSDSQSWEDIAPEIEPCAENRLDTKEEKSVEYEGDLKSGTAETEPVEQDSSQPSLPLVRADDPLRLDQELQQPQTQEKTSNSLTEDSKMANAKQLSSDAEAQKLGKPSAHASQSFCESSSETPFHFTLPKEGDIIPPLTGATPPLIGHLKLEPKRHSTPIGISNYPESTIATSDVMSESMVETHDPILGSGKGDSGAAPDVDDKLCLRMKLVSPETEASEESLQFNLEKPATGERKNGSTAVAESVASPQKTMSVLSCICEARQENEARSEDPPTTPIRGNLLHFPSSQGEEEKEKLEGDHTIRQSQQPMKPISPVKDPVSPASQKMVIQGPSSPQGEAMVTDVLEDQKEGRSTNKENPSKALIERPSQNNIGIQTMECSLRVPETVSAATQTIKNVCEQGTSTVDQNFGKQDATVQTERGSGEKPVSAPGDDTESLHSQGEEEFDMPQPPHGHVLHRHMRTIREVRTLVTRVITDVYYVDGTEVERKVTEETEEPIVECQECETEVSPSQTGGSSGDLGDISSFSSKASSLHRTSSGTSLSAMHSSGSSGKGAGPLRGKTSGTEPADFALPSSRGGPGKLSPRKGVSQTGTPVCEEDGDAGLGIRQGGKAPVTPRGRGRRGRPPSRTTGTRETAVPGPLGIEDISPNLSPDDKSFSRVVPRVPDSTRRTDVGAGALRRSDSPEIPFQAAAGPSDGLDASSPGNSFVGLRVVAKWSSNGYFYSGKITRDVGAGKYKLLFDDGYECDVLGKDILLCDPIPLDTEVTALSEDEYFSAGVVKGHRKESGELYYSIEKEGQRKWYKRMAVILSLEQGNRLREQYGLGPYEAVTPLTKAADISLDNLVEGKRKRRSNVSSPATPTASSSSSTTPTRKITESPRASMGVLSGKRKLITSEEERSPAKRGRKSATVKPGAVGAGEFVSPCESGDNTGEPSALEEQRGPLPLNKTLFLGYAFLLTMATTSDKLASRSKLPDGPTGSSEEEEEFLEIPPFNKQYTESQLRAGAGYILEDFNEAQCNTAYQCLLIADQHCRTRKYFLCLASGIPCVSHVWVHDSCHANQLQNYRNYLLPAGYSLEEQRILDWQPRENPFQNLKVLLVSDQQQNFLELWSEILMTGGAASVKQHHSSAHNKDIALGVFDVVVTDPSCPASVLKCAEALQLPVVSQEWVIQCLIVGERIGFKQHPKYKHDYVSH

PTMs - Q12888 As Substrate

Site PTM Type Enzyme
Sumoylation
S6 Phosphorylation Q13315 (ATM)
S25 Phosphorylation Q13315 (ATM)
S29 Phosphorylation Q13315 (ATM)
K54 Acetylation
K54 Ubiquitination
S63 Phosphorylation
S78 Phosphorylation
T100 Phosphorylation
S103 Phosphorylation
S105 Phosphorylation
T117 Phosphorylation
S119 Phosphorylation
S124 Phosphorylation
S127 Phosphorylation
K135 Methylation
S166 Phosphorylation
S176 Phosphorylation
S178 Phosphorylation
Y188 Phosphorylation
S202 Phosphorylation
S208 Phosphorylation
T214 Phosphorylation
K217 Acetylation
K217 Sumoylation
S222 Phosphorylation
S232 Phosphorylation
K234 Ubiquitination
K244 Acetylation
K244 Ubiquitination
S259 Phosphorylation
S265 Phosphorylation
S280 Phosphorylation
S287 Phosphorylation
S294 Phosphorylation
S301 Phosphorylation
T302 Phosphorylation Q13315 (ATM)
S310 Phosphorylation
T313 Phosphorylation
S315 Phosphorylation
S316 Phosphorylation
S320 Phosphorylation
T321 Phosphorylation
S323 Phosphorylation
T334 Phosphorylation
T337 Phosphorylation
S349 Phosphorylation
S359 Phosphorylation
S360 Phosphorylation
S366 Phosphorylation
T373 Phosphorylation
S379 Phosphorylation
S380 Phosphorylation
T382 Phosphorylation
T394 Phosphorylation
S395 Phosphorylation
S398 Phosphorylation
S452 Phosphorylation
S482 Phosphorylation
S483 Phosphorylation
S484 Phosphorylation
S493 Phosphorylation
S500 Phosphorylation
S507 Phosphorylation
T509 Phosphorylation
S512 Phosphorylation
C513 S-Nitrosylation
S518 Phosphorylation
T519 Phosphorylation
S520 Phosphorylation
Y522 Phosphorylation
S523 Phosphorylation
S525 Phosphorylation
K527 Acetylation
K527 Ubiquitination
S530 Phosphorylation
S532 Phosphorylation
S533 Phosphorylation
T543 Phosphorylation
T548 Phosphorylation
S552 Phosphorylation
S557 Phosphorylation
S566 Phosphorylation
S580 Phosphorylation
T586 Phosphorylation
T591 Phosphorylation
T593 Phosphorylation
S598 Phosphorylation
S625 Phosphorylation
S630 Phosphorylation
S635 Phosphorylation
S639 Phosphorylation
S640 Phosphorylation
S659 Phosphorylation
S660 Phosphorylation
S662 Phosphorylation
T670 Phosphorylation
S674 Phosphorylation
K680 Sumoylation
S686 Phosphorylation
S692 Phosphorylation
T696 Phosphorylation
S698 Phosphorylation
S724 Phosphorylation
S727 Phosphorylation
T750 Phosphorylation
S751 Phosphorylation
S754 Phosphorylation
S755 Phosphorylation
S765 Phosphorylation
S771 Phosphorylation
S782 Phosphorylation
S784 Phosphorylation Q13315 (ATM)
S786 Phosphorylation
T804 Phosphorylation
K808 Ubiquitination
S809 Phosphorylation
Y812 Phosphorylation
T820 Phosphorylation
T823 Phosphorylation
S830 Phosphorylation
S831 Phosphorylation Q13315 (ATM)
S834 Phosphorylation
T855 Phosphorylation
T859 Phosphorylation
S860 Phosphorylation
S862 Phosphorylation
S867 Phosphorylation
K868 Sumoylation
K873 Ubiquitination
S876 Phosphorylation
S877 Phosphorylation
S892 Phosphorylation
S894 Phosphorylation
S898 Phosphorylation
S899 Phosphorylation
S900 Phosphorylation
T919 Phosphorylation
T922 Phosphorylation
K930 Sumoylation
T938 Phosphorylation
T952 Phosphorylation
S970 Phosphorylation
S975 Phosphorylation
K984 Ubiquitination
S993 Phosphorylation
T996 Phosphorylation
S999 Phosphorylation
S1002 Phosphorylation
K1009 Ubiquitination
T1012 Phosphorylation
T1020 Phosphorylation
S1025 Phosphorylation
S1028 Phosphorylation
T1032 Phosphorylation
S1034 Phosphorylation
S1037 Phosphorylation
S1050 Phosphorylation
T1055 Phosphorylation
T1056 Phosphorylation
S1067 Phosphorylation
S1068 Phosphorylation
S1086 Phosphorylation
S1094 Phosphorylation
S1101 Phosphorylation
S1104 Phosphorylation
S1113 Phosphorylation
S1114 Phosphorylation
T1122 Phosphorylation
S1148 Phosphorylation
T1156 Phosphorylation
S1160 Phosphorylation
R1162 Methylation
T1166 Phosphorylation
T1171 Phosphorylation
K1175 Methylation
S1202 Phosphorylation
S1208 Phosphorylation
T1214 Phosphorylation
S1216 Phosphorylation
S1219 Phosphorylation Q13315 (ATM)
Y1258 Phosphorylation
Y1259 Phosphorylation
T1263 Phosphorylation
S1288 Phosphorylation
S1290 Phosphorylation
T1292 Phosphorylation
T1315 Phosphorylation
S1316 Phosphorylation
S1317 Phosphorylation
T1319 Phosphorylation
S1320 Phosphorylation
S1327 Phosphorylation
S1329 Phosphorylation
S1330 Phosphorylation
K1332 Acetylation
K1340 Ubiquitination
T1341 Phosphorylation
S1342 Phosphorylation
T1344 Phosphorylation
S1354 Phosphorylation
R1355 Methylation
S1362 Phosphorylation
S1368 Phosphorylation
T1370 Phosphorylation
T1372 Phosphorylation
C1375 S-Nitrosylation
K1390 Acetylation
T1394 Phosphorylation
R1396 Methylation
R1398 Methylation
R1400 Methylation
R1401 Methylation
R1403 Methylation
S1426 Phosphorylation
S1430 Phosphorylation
K1434 Sumoylation
S1435 Phosphorylation
S1437 Phosphorylation
T1450 Phosphorylation
S1460 Phosphorylation
S1462 Phosphorylation
S1474 Phosphorylation
S1480 Phosphorylation
S1481 Phosphorylation
S1485 Phosphorylation
S1496 Phosphorylation
Y1500 Phosphorylation
K1505 Acetylation
K1516 Ubiquitination
Y1523 Phosphorylation
S1548 Phosphorylation
Y1552 Phosphorylation
S1554 Phosphorylation
K1559 Ubiquitination
K1563 Sumoylation
K1563 Ubiquitination
Y1569 Phosphorylation
K1574 Ubiquitination
Y1605 Phosphorylation
T1609 Phosphorylation P06493 (CDK1) , P24941 (CDK2)
T1612 Phosphorylation P24941 (CDK2)
K1613 Ubiquitination
S1618 Phosphorylation P53350 (PLK1)
K1626 Acetylation
K1626 Methylation
K1626 Ubiquitination
S1631 Phosphorylation
S1634 Phosphorylation
S1635 Phosphorylation
T1638 Phosphorylation
T1640 Phosphorylation
S1642 Phosphorylation
S1643 Phosphorylation
S1644 Phosphorylation
S1645 Phosphorylation
S1646 Phosphorylation
T1647 Phosphorylation
T1648 Phosphorylation
T1654 Phosphorylation
S1656 Phosphorylation
S1660 Phosphorylation
S1665 Phosphorylation
K1667 Acetylation
T1672 Phosphorylation
S1673 Phosphorylation
S1678 Phosphorylation O14757 (CHEK1) , P06493 (CDK1)
S1686 Phosphorylation
K1690 Ubiquitination
S1701 Phosphorylation
S1705 Phosphorylation
T1709 Phosphorylation
S1713 Phosphorylation
S1749 Phosphorylation
T1756 Phosphorylation
S1758 Phosphorylation
S1759 Phosphorylation
K1773 Ubiquitination
S1778 Phosphorylation Q13315 (ATM)
S1929 Phosphorylation
K1960 Ubiquitination
K1966 Ubiquitination
S1971 Phosphorylation

Research Backgrounds

Function:

Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis. Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1. In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites. Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites. Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs. Participates to the repair and the orientation of the broken DNA ends during CSR (By similarity). In contrast, it is not required for classic NHEJ and V(D)J recombination (By similarity). Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1.

PTMs:

Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.

Phosphorylated at basal level in the absence of DNA damage. Phosphorylated by ATM in response to DNA damage: phosphorylation at different sites promotes interaction with different set of proteins: phosphorylation at the N-terminus by ATM (residues from 6-178) promotes interaction with PAXIP1 and non-homologous end joining (NHEJ) of dysfunctional telomeres. Phosphorylation by ATM at residues that are located more C-terminus (residues 300-650) leads to promote interaction with RIF1. Interaction with RIF1 leads to disrupt interaction with NUDT16L1/TIRR. Phosphorylation at Thr-1609 and Ser-1618 in the UDR motif blocks interaction with H2AK15ub. Dephosphorylated by PPP4C. Hyperphosphorylation during mitosis correlates with its exclusion from chromatin and DNA lesions. Hyperphosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation. Dephosphorylated by PPP5C.

Subcellular Location:

Nucleus. Chromosome. Chromosome>Centromere>Kinetochore.
Note: Localizes to the nucleus in absence of DNA damage (PubMed:28241136). Following DNA damage, recruited to sites of DNA damage, such as double stand breaks (DSBs): recognizes and binds histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:23333306, PubMed:23760478, PubMed:24703952, PubMed:28241136, PubMed:17190600). Associated with kinetochores during mitosis (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homoligomer. Interacts with p53/TP53 (via the central domain). Interacts with DCLRE1C. Interacts with histone H2AX and this requires phosphorylation of H2AX on 'Ser-139'. Interacts with histone H4 that has been dimethylated at 'Lys-20' (H4K20me2). Has low affinity for histone H4 containing monomethylated 'Lys-20' (H4K20me1). Does not bind histone H4 containing unmethylated or trimethylated 'Lys-20' (H4K20me3). Has low affinity for histone H3 that has been dimethylated on 'Lys-79'. Has very low affinity for histone H3 that has been monomethylated on 'Lys-79' (in vitro). Does not bind unmethylated histone H3. Interacts with histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub). Interacts with PWWP3A/EXPAND1. Interacts with CHEK2; modulates CHEK2 phosphorylation at 'Thr-68' in response to infrared. Interacts with MSL1; this interaction may be required for MSL1 DNA repair activity, but not for histone acetyltransferase activity. Interacts (when phosphorylated by ATM) with RIF1. Interacts (via the Tudor-like domain) with NUDT16L1/TIRR; interaction masks the Tudor-like domain and prevents recruitment to chromatin. Interacts with PAXIP1. Interacts with IFI202A (By similarity). Interacts with SHLD2.

Family&Domains:

The Tudor-like region mediates binding to histone H4 dimethylated at 'Lys-20' (H4K20me2) (PubMed:17190600). Interaction with NUDT16L1/TIRR masks the Tudor-like domain and prevents recruitment to chromatin (PubMed:28241136).

The UDR (ubiquitin-dependent recruitment) motif specifically recognizes and binds histone H2A monoubiquitinated at 'Lys-15' (H2AK15ub) (PubMed:23760478, PubMed:24703952). Phosphorylation of the UDR blocks interaction with H2AK15ub (PubMed:24703952).

Research Fields

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

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