Phospho-WEE1 (Ser123) Antibody | Affinity Biosciences

IF/ICC

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AF3815 staining Hela cells(heat shock treatment) by IF/ICC.

Product:	Phospho-WEE1 (Ser123) Antibody
Catalog:	AF3815
Description:	Rabbit polyclonal antibody to Phospho-WEE1 (Ser123)
Application:	IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	55kD(isoform), 72~100kD; 72kD(Calculated).
Uniprot:	P30291
RRID:	AB_2847129

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Phospho-WEE1 (Ser123) Antibody detects endogenous levels of WEE1 only when phosphorylated at Ser123.

RRID:

AB_2847129
Cite Format: Affinity Biosciences Cat# AF3815, RRID:AB_2847129.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DKFZp686I18166; EC 2.7.10.2; FLJ16446; MGC105683; OTTHUMP00000231338; OTTHUMP00000231339; Wee 1; WEE 1 homolog 1 (S. pombe); WEE1; WEE1 homolog (S. pombe); Wee1 homolog; WEE1 homolog S. pombe; Wee1 like protein kinase; Wee1 tyrosine kinase; Wee1+ homolog; Wee1+ S. pombe homolog; WEE1, S. pombe, homolog of; WEE1, somatic; Wee1-like protein kinase; WEE1_HUMAN; WEE1A; Wee1A kinase; WEE1hu;

Immunogens

Immunogen:

A synthesized peptide derived from human WEE1 around the phosphorylation site of Ser123.

Uniprot:

P30291(WEE1_HUMAN) >>Visit HPA database.

Gene(ID):

WEE1(7465)

Sequence:

P30291 WEE1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSFLSRQQPPPPRRAGAACTLRQKLIFSPCSDCEEEEEEEEEEGSGHSTGEDSAFQEPDSPLPPARSPTEPGPERRRSPGPAPGSPGELEEDLLLPGACPGADEAGGGAEGDSWEEEGFGSSSPVKSPAAPYFLGSSFSPVRCGGPGDASPRGCGARRAGEGRRSPRPDHPGTPPHKTFRKLRLFDTPHTPKSLLSKARGIDSSSVKLRGSSLFMDTEKSGKREFDVRQTPQVNINPFTPDSLLLHSSGQCRRRKRTYWNDSCGEDMEASDYELEDETRPAKRITITESNMKSRYTTEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAVLGQHSHVVRYFSAWAEDDHMLIQNEYCNGGSLADAISENYRIMSYFKEAELKDLLLQVGRGLRYIHSMSLVHMDIKPSNIFISRTSIPNAASEEGDEDDWASNKVMFKIGDLGHVTRISSPQVEEGDSRFLANEVLQENYTHLPKADIFALALTVVCAAGAEPLPRNGDQWHEIRQGRLPRIPQVLSQEFTELLKVMIHPDPERRPSAMALVKHSVLLSASRKSAEQLRIELNAEKFKNSLLQKELKKAQMAKAAAEERALFTDRMATRSTTQSNRTSRLIGKKMNRSVSLTIY

PTMs - P30291 As Substrate

P30291

Site	PTM Type	Enzyme	Source
	Phosphorylation		Uniprot
R14	Methylation		Uniprot
S48	Phosphorylation		Uniprot
T49	Phosphorylation		Uniprot
S53	Phosphorylation	P53350 (PLK1)	Uniprot
S67	Phosphorylation		Uniprot
T69	Phosphorylation		Uniprot
S78	Phosphorylation		Uniprot
S85	Phosphorylation		Uniprot
S121	Phosphorylation	P68400 (CSNK2A1)	Uniprot
S123	Phosphorylation	P53350 (PLK1) , P06493 (CDK1) , P24941 (CDK2)	Uniprot
S127	Phosphorylation		Uniprot
Y132	Phosphorylation		Uniprot
S137	Phosphorylation		Uniprot
S139	Phosphorylation		Uniprot
S150	Phosphorylation		Uniprot
S165	Phosphorylation		Uniprot
T173	Phosphorylation		Uniprot
T187	Phosphorylation		Uniprot
T190	Phosphorylation		Uniprot
S193	Phosphorylation		Uniprot
S196	Phosphorylation		Uniprot
R199	Methylation		Uniprot
K207	Ubiquitination		Uniprot
S211	Phosphorylation	P49841 (GSK3B)	Uniprot
S212	Phosphorylation	P48730 (CSNK1D)	Uniprot
T217	Phosphorylation		Uniprot
T239	Phosphorylation		Uniprot
S242	Phosphorylation		Uniprot
Y258	Phosphorylation		Uniprot
S262	Phosphorylation		Uniprot
S270	Phosphorylation		Uniprot
Y272	Phosphorylation		Uniprot
K292	Ubiquitination		Uniprot
S293	Phosphorylation		Uniprot
K304	Ubiquitination		Uniprot
S307	Phosphorylation		Uniprot
S312	Phosphorylation		Uniprot
K315	Ubiquitination		Uniprot
S444	Phosphorylation		Uniprot
K460	Ubiquitination		Uniprot
S472	Phosphorylation		Uniprot
S480	Phosphorylation		Uniprot
K547	Ubiquitination		Uniprot
S571	Phosphorylation		Uniprot
S576	Phosphorylation		Uniprot
K588	Ubiquitination		Uniprot
K590	Sumoylation		Uniprot
K590	Ubiquitination		Uniprot
K596	Ubiquitination		Uniprot
K600	Ubiquitination		Uniprot
R617	Methylation		Uniprot
S642	Phosphorylation	Q8IWQ3 (BRSK2) , Q8TDC3 (BRSK1) , P31749 (AKT1)	Uniprot
Y646	Phosphorylation		Uniprot

PTMs - P30291 As Enzyme

P30291

Substrate	Site	Source
P06493-2 (CDK1)	Y15	Uniprot
P07900 (HSP90AA1)	Y38	Uniprot
P24941 (CDK2)	T14	Uniprot
P24941-1 (CDK2)	Y15	Uniprot
P30291-1 (WEE1)	T173	Uniprot

Research Backgrounds

P30291_WEE1_HUMAN

Function:

Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

PTMs:

Phosphorylated during M and G1 phases. Also autophosphorylated. Phosphorylation at Ser-642 by BRSK1 and BRSK2 in post-mitotic neurons, leads to down-regulate WEE1 activity in polarized neurons. Phosphorylated at Ser-53 and Ser-123 by PLK1 and CDK1, respectively, generating an signal for degradation that can be recognized by the SCF(BTRC) complex, leading to its ubiquitination and degradation at the onset of G2/M phase.

Dephosphorylated at Thr-239 by CTDP1.

Ubiquitinated and degraded at the onset of G2/M phase.

Subcellular Location:

Nucleus.

Family&Domains:

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)

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Phospho-WEE1 (Ser123) Antibody - #AF3815