Phospho-MyoD1 (Ser200) Antibody - #AF3799
| Product: | Phospho-MyoD1 (Ser200) Antibody |
| Catalog: | AF3799 |
| Description: | Rabbit polyclonal antibody to Phospho-MyoD1 (Ser200) |
| Application: | IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Mol.Wt.: | 35kD(Calculated). |
| Uniprot: | P15172 |
| RRID: | AB_2847113 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF3799, RRID:AB_2847113.
Fold/Unfold
bHLHc1; Class C basic helix-loop-helix protein 1; MYF 3; Myf-3; MYF3; Myoblast determination protein 1; Myod 1; MYOD; MYOD1; MYOD1_HUMAN; Myogenic differentiation 1; Myogenic factor 3; Myogenic factor MYF 3; Myogenin D1; PUM;
Immunogens
A synthesized peptide derived from human MyoD1 around the phosphorylation site of Ser200.
- P15172 MYOD1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MELLSPPLRDVDLTAPDGSLCSFATTDDFYDDPCFDSPDLRFFEDLDPRLMHVGALLKPEEHSHFPAAVHPAPGAREDEHVRAPSGHHQAGRCLLWACKACKRKTTNADRRKAATMRERRRLSKVNEAFETLKRCTSSNPNQRLPKVEILRNAIRYIEGLQALLRDQDAAPPGAAAAFYAPGPLPPGRGGEHYSGDSDASSPRSNCSDGMMDYSGPPSGARRRNCYEGAYYNEAPSEPRPGKSAAVSSLDCLSSIVERISTESPAAPALLLADVPSESPPRRQEAAAPSEGESSGDPTQSPDAAPQCPAGANPNPIYQVL
PTMs - P15172 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S5 | Phosphorylation | P06493 (CDK1) | Uniprot |
| Y30 | Phosphorylation | P00519 (ABL1) | Uniprot |
| K58 | Acetylation | Uniprot | |
| K99 | Acetylation | Uniprot | |
| K102 | Acetylation | Uniprot | |
| K104 | Acetylation | Uniprot | |
| K104 | Methylation | Uniprot | |
| T115 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| Y156 | Phosphorylation | Q02750 (MAP2K1) | Uniprot |
| S200 | Phosphorylation | P53778 (MAPK12) , P06493 (CDK1) , P24941 (CDK2) | Uniprot |
| S201 | Phosphorylation | P53778 (MAPK12) | Uniprot |
| S204 | Phosphorylation | Uniprot | |
| S207 | Phosphorylation | Uniprot | |
| Y213 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot |
Research Backgrounds
Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).
Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation.
Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function (By similarity).
Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity.
Nucleus.
Efficient DNA binding requires dimerization with another bHLH protein. Seems to form active heterodimers with ITF-2. Interacts with SUV39H1. Interacts with DDX5. Interacts with CHD2. Interacts with TSC22D3 (By similarity). Interacts with SETD3 (By similarity). Interacts with P-TEFB complex; promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation (By similarity). Interacts with CSRP3. Interacts with NUPR1 (By similarity).
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