Phospho-p23 (Ser113) Antibody | Affinity Biosciences

IF/ICC

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AF3794 staining Hela cells(heat shock treatment) by IF/ICC.

Product:	Phospho-p23 (Ser113) Antibody
Catalog:	AF3794
Description:	Rabbit polyclonal antibody to Phospho-p23 (Ser113)
Application:	IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	19kD(Calculated).
Uniprot:	Q15185
RRID:	AB_2847108

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Phospho-p23 (Ser113) Antibody detects endogenous levels of p23 only when phosphorylated at Ser113.

RRID:

AB_2847108
Cite Format: Affinity Biosciences Cat# AF3794, RRID:AB_2847108.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Co chaperone p23; cPGES; Cytosolic prostaglandin E synthase; Cytosolic prostaglandin E2 synthase; Hsp90 co chaperone; Hsp90 co-chaperone; P23; Progesterone receptor complex; Progesterone receptor complex p23; Prostaglandin E synthase 3 (cytosolic); Prostaglandin E synthase 3; PTGES 3; PTGES3; Sid 3177; TEBP; TEBP_HUMAN; Telomerase binding protein p23; Telomerase-binding protein p23; Unactive progesterone receptor 23 kD;

Immunogens

Immunogen:

A synthesized peptide derived from human p23 around the phosphorylation site of Ser113.

Uniprot:

Q15185(TEBP_HUMAN) >>Visit HPA database.

Gene(ID):

PTGES3(10728)

Sequence:

Q15185 TEBP_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MQPASAKWYDRRDYVFIEFCVEDSKDVNVNFEKSKLTFSCLGGSDNFKHLNEIDLFHCIDPNDSKHKRTDRSILCCLRKGESGQSWPRLTKERAKLNWLSVDFNNWKDWEDDSDEDMSNFDRFSEMMNNMGGDEDVDLPEVDGADDDSQDSDDEKMPDLE

PTMs - Q15185 As Substrate

Q15185

Site	PTM Type	Enzyme	Source
K7	Acetylation		Uniprot
K7	Ubiquitination		Uniprot
Y14	Phosphorylation		Uniprot
K33	Acetylation		Uniprot
K33	Methylation		Uniprot
K33	Sumoylation		Uniprot
K33	Ubiquitination		Uniprot
K35	Acetylation		Uniprot
K35	Sumoylation		Uniprot
K35	Ubiquitination		Uniprot
S39	Phosphorylation		Uniprot
C40	S-Nitrosylation		Uniprot
S44	Phosphorylation		Uniprot
K48	Acetylation		Uniprot
K48	Methylation		Uniprot
K48	Ubiquitination		Uniprot
C58	S-Nitrosylation		Uniprot
S64	Phosphorylation		Uniprot
K65	Sumoylation		Uniprot
K65	Ubiquitination		Uniprot
K67	Ubiquitination		Uniprot
S72	Phosphorylation		Uniprot
C75	S-Nitrosylation		Uniprot
C76	S-Nitrosylation		Uniprot
K79	Sumoylation		Uniprot
K79	Ubiquitination		Uniprot
S82	Phosphorylation		Uniprot
S85	Phosphorylation		Uniprot
K91	Acetylation		Uniprot
K91	Ubiquitination		Uniprot
K95	Sumoylation		Uniprot
K95	Ubiquitination		Uniprot
S100	Phosphorylation		Uniprot
S113	Phosphorylation	P68400 (CSNK2A1)	Uniprot
S118	Phosphorylation	P68400 (CSNK2A1)	Uniprot
S124	Phosphorylation		Uniprot
S148	Phosphorylation		Uniprot
S151	Phosphorylation		Uniprot

Research Backgrounds

Q15185_TEBP_HUMAN

Function:

Cytosolic prostaglandin synthase that catalyzes the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). Molecular chaperone that localizes to genomic response elements in a hormone-dependent manner and disrupts receptor-mediated transcriptional activation, by promoting disassembly of transcriptional regulatory complexes. Facilitates HIF alpha proteins hydroxylation via interaction with EGLN1/PHD2, leading to recruit EGLN1/PHD2 to the HSP90 pathway.

Subcellular Location:

Cytoplasm.

Subunit Structure:

Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, CDC37, PPP5C, PTGES3/p23, TSC1 and client protein TSC2. Binds to the progesterone receptor. Interacts with TERT; the interaction, together with HSP90AA1, is required for correct assembly and stabilization of the telomerase holoenzyme complex. Interacts (via PXLE motif) with EGLN1/PHD2, recruiting EGLN1/PHD2 to the HSP90 pathway to facilitate HIF alpha proteins hydroxylation. Interacts with HSP90AA1, FLCN, FNIP1 and FNIP2.

Family&Domains:

The PXLE motif mediates interaction with EGLN1/PHD2.

Belongs to the p23/wos2 family.

Research Fields

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

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For Research Use Only.
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Phospho-p23 (Ser113) Antibody - #AF3794