Phospho-SGK2 (Thr253) Antibody - #AF3791

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Product: Phospho-SGK2 (Thr253) Antibody
Catalog: AF3791
Description: Rabbit polyclonal antibody to Phospho-SGK2 (Thr253)
Application: WB IHC
Reactivity: Human, Rat
Mol.Wt.: 48kD(Calculated).
Uniprot: Q9HBY8
RRID: AB_2847105

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Clonality:
Polyclonal
Specificity:
Phospho-SGK2 (Thr253) Antibody detects endogenous levels of SGK2 only when phosphorylated at Thr253.
RRID:
AB_2847105
Cite Format: Affinity Biosciences Cat# AF3791, RRID:AB_2847105.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DJ138B7.2; H SGK2; HSGK2; Serine/threonine protein kinase SGK2; Serine/threonine-protein kinase Sgk2; Serum/glucocorticoid regulated kinase 2; Serum/glucocorticoid-regulated kinase 2; SGK2; SGK2_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human SGK2 around the phosphorylation site of Thr253.

Uniprot:

Q9HBY8(SGK2_HUMAN) >>Visit HPA database.

Gene(ID):
SGK2(10110)
Expression:
Q9HBY8 SGK2_HUMAN:

Highly expressed in liver, kidney and pancreas, and at lower levels in brain.

Sequence:
MQGLLTSGRKPSGGGRCTGRGGWRGQWCLKPWMGGADPPTPTLSCLLLPVPPELPDHCYRMNSSPAGTPSPQPSRANGNINLGPSANPNAQPTDFDFLKVIGKGNYGKVLLAKRKSDGAFYAVKVLQKKSILKKKEQSHIMAERSVLLKNVRHPFLVGLRYSFQTPEKLYFVLDYVNGGELFFHLQRERRFLEPRARFYAAEVASAIGYLHSLNIIYRDLKPENILLDCQGHVVLTDFGLCKEGVEPEDTTSTFCGTPEYLAPEVLRKEPYDRAVDWWCLGAVLYEMLHGLPPFYSQDVSQMYENILHQPLQIPGGRTVAACDLLQSLLHKDQRQRLGSKADFLEIKNHVFFSPINWDDLYHKRLTPPFNPNVTGPADLKHFDPEFTQEAVSKSIGCTPDTVASSSGASSAFLGFSYAPEDDDILDC

Research Backgrounds

Function:

Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, survival and proliferation. Up-regulates Na(+) channels: SCNN1A/ENAC, K(+) channels: KCNA3/Kv1.3, KCNE1 and KCNQ1, amino acid transporter: SLC6A19, glutamate transporter: SLC1A6/EAAT4, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na(+)/H(+) exchanger: SLC9A3/NHE3, and the Na(+)/K(+) ATPase.

PTMs:

Activated by phosphorylation on Ser-416 by an unknown kinase (may be mTORC2 but not confirmed), transforming it into a substrate for PDPK1 which then phosphorylates it on Thr-253.

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in liver, kidney and pancreas, and at lower levels in brain.

Family&Domains:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Research Fields

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

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