Product: Phospho-Dnmt1 (Tyr399) Antibody
Catalog: AF3790
Description: Rabbit polyclonal antibody to Phospho-Dnmt1 (Tyr399)
Application: WB
Reactivity: Human
Mol.Wt.: 150kDa; 183kD(Calculated).
Uniprot: P26358
RRID: AB_2847104

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
Phospho-Dnmt1 (Tyr399) Antibody detects endogenous levels of Dnmt1 only when phosphorylated at Tyr399.
RRID:
AB_2847104
Cite Format: Affinity Biosciences Cat# AF3790, RRID:AB_2847104.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ADCADN; AIM; CXXC finger protein 9; CXXC-type zinc finger protein 9; CXXC9; DNA (cytosine 5 ) methyltransferase 1; DNA (cytosine-5)-methyltransferase 1; DNA methyltransferase 1; DNA methyltransferase HsaI; DNA methyltransferase M.HsaI.; DNA MTase; DNA MTase HsaI; DNMT 1; DNMT; Dnmt1; DNMT1_HUMAN; Dnmt1o; FLJ16293; HSN1E; M.HsaI; MCMT; Met1; MGC104992; mMmul; MommeD2;

Immunogens

Immunogen:

A synthesized peptide derived from human Dnmt1 around the phosphorylation site of Tyr399.

Uniprot:
Gene(ID):
Expression:
P26358 DNMT1_HUMAN:

Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.

Sequence:
MPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYNREVNGRLENGNQARSEARRVGMADANSPPKPLSKPRTPRRSKSDGEAKPEPSPSPRITRKSTRQTTITSHFAKGPAKRKPQEESERAKSDESIKEEDKDQDEKRRRVTSRERVARPLPAEEPERAKSGTRTEKEEERDEKEEKRLRSQTKEPTPKQKLKEEPDREARAGVQADEDEDGDEKDEKKHRSQPKDLAAKRRPEEKEPEKVNPQISDEKDEDEKEEKRRKTTPKEPTEKKMARAKTVMNSKTHPPKCIQCGQYLDDPDLKYGQHPPDAVDEPQMLTNEKLSIFDANESGFESYEALPQHKLTCFSVYCKHGHLCPIDTGLIEKNIELFFSGSAKPIYDDDPSLEGGVNGKNLGPINEWWITGFDGGEKALIGFSTSFAEYILMDPSPEYAPIFGLMQEKIYISKIVVEFLQSNSDSTYEDLINKIETTVPPSGLNLNRFTEDSLLRHAQFVVEQVESYDEAGDSDEQPIFLTPCMRDLIKLAGVTLGQRRAQARRQTIRHSTREKDRGPTKATTTKLVYQIFDTFFAEQIEKDDREDKENAFKRRRCGVCEVCQQPECGKCKACKDMVKFGGSGRSKQACQERRCPNMAMKEADDDEEVDDNIPEMPSPKKMHQGKKKKQNKNRISWVGEAVKTDGKKSYYKKVCIDAETLEVGDCVSVIPDDSSKPLYLARVTALWEDSSNGQMFHAHWFCAGTDTVLGATSDPLELFLVDECEDMQLSYIHSKVKVIYKAPSENWAMEGGMDPESLLEGDDGKTYFYQLWYDQDYARFESPPKTQPTEDNKFKFCVSCARLAEMRQKEIPRVLEQLEDLDSRVLYYSATKNGILYRVGDGVYLPPEAFTFNIKLSSPVKRPRKEPVDEDLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIFCPKKSNGRPNETDIKIRVNKFYRPENTHKSTPASYHADINLLYWSDEEAVVDFKAVQGRCTVEYGEDLPECVQVYSMGGPNRFYFLEAYNAKSKSFEDPPNHARSPGNKGKGKGKGKGKPKSQACEPSEPEIEIKLPKLRTLDVFSGCGGLSEGFHQAGISDTLWAIEMWDPAAQAFRLNNPGSTVFTEDCNILLKLVMAGETTNSRGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSFKRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEVRNGASALEISYNGEPQSWFQRQLRGAQYQPILRDHICKDMSALVAARMRHIPLAPGSDWRDLPNIEVRLSDGTMARKLRYTHHDRKNGRSSSGALRGVCSCVEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVGNAVPPPLAKAIGLEIKLCMLAKARESASAKIKEEEAAKD

PTMs - P26358 As Substrate

Site PTM Type Enzyme
K45 Ubiquitination
K58 Ubiquitination
K67 Ubiquitination
K70 Methylation
K70 Ubiquitination
K81 Ubiquitination
K83 Ubiquitination
S84 Phosphorylation
K88 Ubiquitination
S127 Phosphorylation
S133 Phosphorylation
K134 Ubiquitination
T137 Phosphorylation
S141 Phosphorylation
K142 Methylation
S143 Phosphorylation P31749 (AKT1)
K148 Sumoylation
S152 Phosphorylation
S154 Phosphorylation P06493 (CDK1) , Q00535 (CDK5) , P24941 (CDK2)
K160 Acetylation
T165 Phosphorylation
T166 Phosphorylation
T168 Phosphorylation
K173 Acetylation
K173 Ubiquitination
K188 Acetylation
S189 Phosphorylation
S192 Phosphorylation
K194 Sumoylation
T208 Phosphorylation
S209 Phosphorylation
S227 Phosphorylation
S247 Phosphorylation
K259 Acetylation
K259 Sumoylation
S288 Phosphorylation
S312 Phosphorylation
T328 Phosphorylation
K335 Acetylation
K336 Ubiquitination
S346 Phosphorylation
Y359 Phosphorylation
K366 Acetylation
K385 Ubiquitination
S394 Phosphorylation
S398 Phosphorylation
Y399 Phosphorylation
S549 Phosphorylation
K586 Ubiquitination
T591 Phosphorylation
K638 Ubiquitination
K644 Ubiquitination
K666 Ubiquitination
K668 Ubiquitination
K675 Sumoylation
K675 Ubiquitination
S714 Phosphorylation P42345 (MTOR)
K716 Ubiquitination
S732 Phosphorylation
K739 Sumoylation
K749 Acetylation
S878 Phosphorylation
K881 Ubiquitination
T882 Phosphorylation
K889 Ubiquitination
K891 Acetylation
S919 Phosphorylation
K928 Ubiquitination
S954 Phosphorylation
K957 Acetylation
K957 Sumoylation
K957 Ubiquitination
K961 Acetylation
K961 Ubiquitination
Y969 Phosphorylation
K975 Acetylation
K975 Ubiquitination
S977 Phosphorylation
K981 Methylation
K981 Ubiquitination
S983 Phosphorylation
K997 Ubiquitination
K1020 Ubiquitination
K1054 Acetylation
K1092 Ubiquitination
K1094 Ubiquitination
S1105 Phosphorylation
K1111 Acetylation
K1113 Acetylation
K1115 Acetylation
K1117 Acetylation
K1119 Acetylation
K1121 Acetylation
S1122 Phosphorylation
K1135 Sumoylation
K1135 Ubiquitination
K1196 Ubiquitination
T1204 Phosphorylation
K1214 Ubiquitination
Y1254 Phosphorylation
K1323 Ubiquitination
K1349 Acetylation
K1349 Ubiquitination
S1358 Phosphorylation
R1401 Methylation
Y1405 Phosphorylation
K1415 Acetylation
K1415 Ubiquitination
S1447 Phosphorylation
R1473 Methylation
K1483 Ubiquitination
S1524 Phosphorylation
T1525 Phosphorylation
T1526 Phosphorylation
K1535 Ubiquitination
K1572 Ubiquitination
K1586 Ubiquitination
K1599 Ubiquitination
S1605 Phosphorylation
K1609 Sumoylation

Research Backgrounds

Function:

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing. Promotes tumor growth.

PTMs:

Sumoylated; sumoylation increases activity.

Acetylation on multiple lysines, mainly by KAT2B/PCAF, regulates cell cycle G(2)/M transition. Deacetylation of Lys-1349 and Lys-1415 by SIRT1 increases methyltransferase activity.

Phosphorylation of Ser-154 by CDKs is important for enzymatic activity and protein stability. Phosphorylation of Ser-143 by AKT1 prevents methylation by SETD7 therebye increasing DNMT1 stability.

Methylation at Lys-142 by SETD7 promotes DNMT1 proteasomal degradation.

Ubiquitinated by UHRF1; interaction with USP7 counteracts ubiquitination by UHRF1 by promoting deubiquitination and preventing degradation by the proteasome.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.

Subunit Structure:

Homodimer. Forms a stable complex with E2F1, BB1 and HDAC1. Forms a complex with DMAP1 and HDAC2, with direct interaction. Interacts with the PRC2/EED-EZH2 complex. Probably part of a corepressor complex containing ZNF304, TRIM28, SETDB1 and DNMT1. Interacts with UHRF1; promoting its recruitment to hemimethylated DNA. Interacts with USP7, promoting its deubiquitination. Interacts with PCNA. Interacts with MBD2 and MBD3. Interacts with DNMT3A and DNMT3B. Interacts with UBC9. Interacts with CSNK1D (By similarity). Interacts with HDAC1 (By similarity). Interacts with BAZ2A/TIP5 (By similarity). Interacts with SIRT7 (By similarity).

Family&Domains:

The N-terminal part is required for homodimerization and acts as a regulatory domain.

The CXXC-type zinc finger specifically binds to unmethylated CpG dinucleotides, positioning the autoinhibitory linker between the DNA and the active site, thus providing a mechanism to ensure that only hemimethylated CpG dinucleotides undergo methylation.

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Research Fields

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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