Phospho-SNAP23 (Ser95) Antibody | Affinity Biosciences

Product:	Phospho-SNAP23 (Ser95) Antibody
Catalog:	AF3788
Description:	Rabbit polyclonal antibody to Phospho-SNAP23 (Ser95)
Application:	ELISA(peptide)
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	23kD(Calculated).
Uniprot:	O00161
RRID:	AB_2847102

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Phospho-SNAP23 (Ser95) Antibody detects endogenous levels of SNAP23 only when phosphorylated at Ser95.

RRID:

AB_2847102
Cite Format: Affinity Biosciences Cat# AF3788, RRID:AB_2847102.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

HsT17016; LS-B8340; SNAP 23; SNAP-23; SNAP23; SNAP23A; SNAP23B; SNP23_HUMAN; Synaptosomal associated protein 23; Synaptosomal associated protein 23kDa; Synaptosomal associated protein; Synaptosomal-associated protein 23; Vesicle membrane fusion protein SNAP 23; Vesicle membrane fusion protein SNAP23; Vesicle-membrane fusion protein SNAP-23;

Immunogens

Immunogen:

A synthesized peptide derived from human SNAP23 around the phosphorylation site of Ser95.

Uniprot:

O00161(SNP23_HUMAN) >>Visit HPA database.

Gene(ID):

SNAP23(8773)

Expression:

O00161 SNP23_HUMAN:

Ubiquitous. Highest levels where found in placenta.

Sequence:

O00161 SNP23_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDNLSSEEIQQRAHQITDESLESTRRILGLAIESQDAGIKTITMLDEQKEQLNRIEEGLDQINKDMRETEKTLTELNKCCGLCVCPCNRTKNFESGKAYKTTWGDGGENSPCNVVSKQPGPVTNGQLQQPTTGAASGGYIKRITNDAREDEMEENLTQVGSILGNLKDMALNIGNEIDAQNPQIKRITDKADTNRDRIDIANARAKKLIDS

PTMs - O00161 As Substrate

O00161

Site	PTM Type	Enzyme	Source
M1	Acetylation		Uniprot
S5	Phosphorylation		Uniprot
S6	Phosphorylation		Uniprot
T17	Phosphorylation		Uniprot
S20	Phosphorylation		Uniprot
S23	Phosphorylation	P17252 (PRKCA)	Uniprot
T24	Phosphorylation	P17252 (PRKCA)	Uniprot
S34	Phosphorylation		Uniprot
K40	Ubiquitination		Uniprot
K49	Ubiquitination		Uniprot
K64	Ubiquitination		Uniprot
K71	Ubiquitination		Uniprot
T72	Phosphorylation		Uniprot
K91	Ubiquitination		Uniprot
S95	Phosphorylation	P14618 (PKM)	Uniprot
K97	Ubiquitination		Uniprot
Y99	Phosphorylation		Uniprot
K100	Ubiquitination		Uniprot
T102	Phosphorylation		Uniprot
S110	Phosphorylation		Uniprot
C112	S-Nitrosylation		Uniprot
K117	Ubiquitination		Uniprot
S136	Phosphorylation		Uniprot
Y139	Phosphorylation		Uniprot
K141	Ubiquitination		Uniprot
T144	Phosphorylation		Uniprot
T157	Phosphorylation		Uniprot
S161	Phosphorylation	P17252 (PRKCA)	Uniprot
K185	Ubiquitination		Uniprot
K190	Ubiquitination		Uniprot

Research Backgrounds

O00161_SNP23_HUMAN

Function:

Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.

Subcellular Location:

Cell membrane>Peripheral membrane protein. Cell membrane>Lipid-anchor. Cell junction>Synapse>Synaptosome.
Note: Mainly localized to the plasma membrane.

Tissue Specificity:

Ubiquitous. Highest levels where found in placenta.

Subunit Structure:

Homotetramer (via coiled-coil domain), also forms heterotetramers with STX4 and VAMP3. Found in a complex with VAMP8 and STX1A. Found in a complex with VAMP8 and STX4 in pancreas (By similarity). Interacts simultaneously with SNAPIN and SYN4 (By similarity). Interacts with STX1A (By similarity). Interacts with STX12 (By similarity). Interacts tightly to multiple syntaxins and synaptobrevins/VAMPs (By similarity). Interacts with ZDHHC13 (via ANK repeats) (By similarity). Interacts with ZDHHC17 (via ANK repeats).

Family&Domains:

Belongs to the SNAP-25 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > SNARE interactions in vesicular transport.

· Organismal Systems > Immune system > Platelet activation. (View pathway)

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Phospho-SNAP23 (Ser95) Antibody - #AF3788