Phospho-SCD1 (Tyr55) Antibody - #AF3781

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Product: Phospho-SCD1 (Tyr55) Antibody
Catalog: AF3781
Description: Rabbit polyclonal antibody to Phospho-SCD1 (Tyr55)
Application: IF/ICC
Reactivity: Human
Mol.Wt.: 41 kDa; 42kD(Calculated).
Uniprot: O00767
RRID: AB_2847095

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
Phospho-SCD1 (Tyr55) Antibody detects endogenous levels of SCD1 only when phosphorylated at Tyr55.
RRID:
AB_2847095
Cite Format: Affinity Biosciences Cat# AF3781, RRID:AB_2847095.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ACOD_HUMAN; Acyl-CoA desaturase; Delta(9)-desaturase; Delta-9 desaturase; Delta-9-Desaturase; FADS5; Fatty acid desaturase; PRO0998; SCD 1; SCD; SCD1; Stearoyl-CoA desaturase;

Immunogens

Immunogen:

A synthesized peptide derived from human SCD1 around the phosphorylation site of Tyr55.

Uniprot:

O00767(ACOD_HUMAN) >>Visit HPA database.

Gene(ID):
SCD(6319)
Expression:
O00767 ACOD_HUMAN:

Detected in fetal liver, lung and brain. Highly expressed in adult adipose tissue, and at lower levels in adult brain and lung.

Sequence:
MPAHLLQDDISSSYTTTTTITAPPSRVLQNGGDKLETMPLYLEDDIRPDIKDDIYDPTYKDKEGPSPKVEYVWRNIILMSLLHLGALYGITLIPTCKFYTWLWGVFYYFVSALGITAGAHRLWSHRSYKARLPLRLFLIIANTMAFQNDVYEWARDHRAHHKFSETHADPHNSRRGFFFSHVGWLLVRKHPAVKEKGSTLDLSDLEAEKLVMFQRRYYKPGLLMMCFILPTLVPWYFWGETFQNSVFVATFLRYAVVLNATWLVNSAAHLFGYRPYDKNISPRENILVSLGAVGEGFHNYHHSFPYDYSASEYRWHINFTTFFIDCMAALGLAYDRKKVSKAAILARIKRTGDGNYKSG

PTMs - O00767 As Substrate

Site PTM Type Enzyme
Y14 Phosphorylation
K34 Ubiquitination
Y41 Phosphorylation
K51 Sumoylation
K51 Ubiquitination
Y55 Phosphorylation
Y59 Phosphorylation
K60 Ubiquitination
K62 Ubiquitination
K68 Sumoylation
K68 Ubiquitination
S80 Phosphorylation
T91 Phosphorylation
T95 Phosphorylation
K162 Ubiquitination
K189 Ubiquitination
K194 Ubiquitination
K196 Ubiquitination
S198 Phosphorylation
T199 Phosphorylation
S203 Phosphorylation
K209 Ubiquitination
K278 Ubiquitination
K338 Ubiquitination
K341 Ubiquitination
T351 Phosphorylation
K357 Ubiquitination

Research Backgrounds

Function:

Stearyl-CoA desaturase that utilizes O(2) and electrons from reduced cytochrome b5 to introduce the first double bond into saturated fatty acyl-CoA substrates. Catalyzes the insertion of a cis double bond at the delta-9 position into fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA. Gives rise to a mixture of 16:1 and 18:1 unsaturated fatty acids. Plays an important role in lipid biosynthesis. Plays an important role in regulating the expression of genes that are involved in lipogenesis and in regulating mitochondrial fatty acid oxidation (By similarity). Plays an important role in body energy homeostasis (By similarity). Contributes to the biosynthesis of membrane phospholipids, cholesterol esters and triglycerides (By similarity).

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in fetal liver, lung and brain. Highly expressed in adult adipose tissue, and at lower levels in adult brain and lung.

Subunit Structure:

May self-associate and form homodimers.

Family&Domains:

The histidine box domains are involved in binding the catalytic metal ions.

Belongs to the fatty acid desaturase type 1 family.

Research Fields

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Metabolism > Lipid metabolism > Biosynthesis of unsaturated fatty acids.

· Metabolism > Global and overview maps > Fatty acid metabolism.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

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