Phospho-HEC1 (Ser165) Antibody - #AF3780

Product: Phospho-HEC1 (Ser165) Antibody
Catalog: AF3780
Description: Rabbit polyclonal antibody to Phospho-HEC1 (Ser165)
Application: ELISA(peptide)
Reactivity: Human, Mouse
Mol.Wt.: 74kD(Calculated).
Uniprot: O14777
RRID: AB_2847094

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
Phospho-HEC1 (Ser165) Antibody detects endogenous levels of HEC1 only when phosphorylated at Ser165.
RRID:
AB_2847094
Cite Format: Affinity Biosciences Cat# AF3780, RRID:AB_2847094.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

from Entrez Gene ;; HEC; Highly expressed in cancer; Highly expressed in cancer protein; Highly expressed in cancer rich in leucine heptad repeats; HsHec1; hsNDC80; Kinetochore associated 2; Kinetochore associated protein 2; Kinetochore protein Hec1; Kinetochore protein NDC80 homolog; Kinetochore-associated protein 2; KNTC2; ndc80; NDC80 homolog kinetochore complex component; NDC80 kinetochore complex component homolog; NDC80, S. cerevisiae, homolog of; NDC80_HUMAN; Retinoblastoma associated protein HEC; Retinoblastoma-associated protein HEC; TID3;

Immunogens

Immunogen:

A synthesized peptide derived from human HEC1 around the phosphorylation site of Ser165.

Uniprot:

O14777(NDC80_HUMAN) >>Visit HPA database.

Gene(ID):
NDC80(10403)
Sequence:
MKRSSVSSGGAGRLSMQELRSQDVNKQGLYTPQTKEKPTFGKLSINKPTSERKVSLFGKRTSGHGSRNSQLGIFSSSEKIKDPRPLNDKAFIQQCIRQLCEFLTENGYAHNVSMKSLQAPSVKDFLKIFTFLYGFLCPSYELPDTKFEEEVPRIFKDLGYPFALSKSSMYTVGAPHTWPHIVAALVWLIDCIKIHTAMKESSPLFDDGQPWGEETEDGIMHNKLFLDYTIKCYESFMSGADSFDEMNAELQSKLKDLFNVDAFKLESLEAKNRALNEQIARLEQEREKEPNRLESLRKLKASLQGDVQKYQAYMSNLESHSAILDQKLNGLNEEIARVELECETIKQENTRLQNIIDNQKYSVADIERINHERNELQQTINKLTKDLEAEQQKLWNEELKYARGKEAIETQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCLVKYRAQVYVPLKELLNETEEEINKALNKKMGLEDTLEQLNAMITESKRSVRTLKEEVQKLDDLYQQKIKEAEEEDEKCASELESLEKHKHLLESTVNQGLSEAMNELDAVQREYQLVVQTTTEERRKVGNNLQRLLEMVATHVGSVEKHLEEQIAKVDREYEECMSEDLSENIKEIRDKYEKKATLIKSSEE

PTMs - O14777 As Substrate

Site PTM Type Enzyme
S5 Phosphorylation Q96GD4 (AURKB)
S15 Phosphorylation Q96GD4 (AURKB)
R20 Methylation
K26 Ubiquitination
Y30 Phosphorylation
T31 Phosphorylation
T34 Phosphorylation
K37 Ubiquitination
T39 Phosphorylation
K42 Ubiquitination
S44 Phosphorylation Q96GD4 (AURKB)
K47 Ubiquitination
T49 Phosphorylation Q96GD4 (AURKB)
S50 Phosphorylation
K53 Acetylation
S55 Phosphorylation Q96GD4 (AURKB) , O14965 (AURKA)
K59 Acetylation
K59 Methylation
K59 Ubiquitination
T61 Phosphorylation
S62 Phosphorylation
S66 Phosphorylation
S69 Phosphorylation Q96GD4 (AURKB)
S76 Phosphorylation
S77 Phosphorylation
K81 Ubiquitination
K89 Ubiquitination
K123 Ubiquitination
R153 Methylation
K156 Ubiquitination
S165 Phosphorylation P51955 (NEK2) , Q96PY6 (NEK1)
S202 Phosphorylation
K253 Ubiquitination
K255 Ubiquitination
K264 Ubiquitination
K271 Ubiquitination
K300 Ubiquitination
K309 Ubiquitination
K327 Ubiquitination
K346 Ubiquitination
K360 Ubiquitination
Y361 Phosphorylation
K382 Ubiquitination
K393 Ubiquitination
K400 Ubiquitination
K405 Ubiquitination
K417 Ubiquitination
K433 Ubiquitination
K452 Ubiquitination
Y458 Phosphorylation
K462 Ubiquitination
K474 Ubiquitination
T502 Phosphorylation
K504 Ubiquitination
K509 Ubiquitination
K517 Ubiquitination
K527 Acetylation
S530 Phosphorylation
T591 Phosphorylation
S595 Phosphorylation
K606 Ubiquitination
S616 Phosphorylation

Research Backgrounds

Function:

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules.

PTMs:

Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB.

Subcellular Location:

Nucleus. Chromosome>Centromere>Kinetochore.
Note: Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end. Interacts with isoform 1 of NEK2 and ZWINT specifically during mitosis. Interacts with CENPH and MIS12. May interact with AURKB, PSMC2, PSMC5 and SMC1A. May interact with RB1 during G2 phase and mitosis. Interacts with CKAP5. Interacts with CDT1; leading to kinetochore localization of CDT1.

Family&Domains:

Belongs to the NDC80/HEC1 family.

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