Phospho-MYPT1 (Ser473) Antibody | Affinity Biosciences

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Western blot analysis of extracts from A549 cells, using Phospho-MYPT1 (Ser473) Antibody.

AF3776 at 1/100 staining Rat heart tissue by IHC-P.

AF3776 at 1/100 staining Rat skin tissue by IHC-P.

Product:	Phospho-MYPT1 (Ser473) Antibody
Catalog:	AF3776
Description:	Rabbit polyclonal antibody to Phospho-MYPT1 (Ser473)
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	150kDa; 115kD(Calculated).
Uniprot:	O14974
RRID:	AB_2847090

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Phospho-MYPT1 (Ser473) Antibody detects endogenous levels of MYPT1 only when phosphorylated at Ser473.

RRID:

AB_2847090
Cite Format: Affinity Biosciences Cat# AF3776, RRID:AB_2847090.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

M130; MBS; Myosin binding subunit; Myosin phosphatase target subunit 1; Myosin phosphatase targeting subunit 1; Myosin phosphatase-targeting subunit 1; MYPT1; MYPT1_HUMAN; PPP1R12A; Protein phosphatase 1 regulatory inhibitor subunit 12A; Protein phosphatase 1 regulatory subunit 12A; Protein phosphatase 1, regulatory (inhibitor) subunit 12A; Protein phosphatase myosin binding subunit; Protein phosphatase myosin-binding subunit;

Immunogens

Immunogen:

A synthesized peptide derived from human MYPT1 around the phosphorylation site of Ser473.

Uniprot:

O14974(MYPT1_HUMAN) >>Visit HPA database.

Gene(ID):

PPP1R12A(4659)

Expression:

O14974 MYPT1_HUMAN:

Expressed in striated muscles, specifically in type 2a fibers (at protein level).

Sequence:

O14974 MYPT1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKMADAKQKRNEQLKRWIGSETDLEPPVVKRQKTKVKFDDGAVFLAACSSGDTDEVLKLLHRGADINYANVDGLTALHQACIDDNVDMVKFLVENGANINQPDNEGWIPLHAAASCGYLDIAEFLIGQGAHVGAVNSEGDTPLDIAEEEAMEELLQNEVNRQGVDIEAARKEEERIMLRDARQWLNSGHINDVRHAKSGGTALHVAAAKGYTEVLKLLIQAGYDVNIKDYDGWTPLHAAAHWGKEEACRILVDNLCDMEMVNKVGQTAFDVADEDILGYLEELQKKQNLLHSEKRDKKSPLIESTANMDNNQSQKTFKNKETLIIEPEKNASRIESLEQEKVDEEEEGKKDESSCSSEEDEEDDSESEAETDKTKPLASVTNANTSSTQAAPVAVTTPTVSSGQATPTSPIKKFPTTATKISPKEEERKDESPATWRLGLRKTGSYGALAEITASKEGQKEKDTAGVTRSASSPRLSSSLDNKEKEKDSKGTRLAYVAPTIPRRLASTSDIEEKENRDSSSLRTSSSYTRRKWEDDLKKNSSVNEGSTYHKSCSFGRRQDDLISSSVPSTTSTPTVTSAAGLQKSLLSSTSTTTKITTGSSSAGTQSSTSNRLWAEDSTEKEKDSVPTAVTIPVAPTVVNAAASTTTLTTTTAGTVSSTTEVRERRRSYLTPVRDEESESQRKARSRQARQSRRSTQGVTLTDLQEAEKTIGRSRSTRTREQENEEKEKEEKEKQDKEKQEEKKESETSREDEYKQKYSRTYDETYQRYRPVSTSSSTTPSSSLSTMSSSLYASSQLNRPNSLVGITSAYSRGITKENEREGEKREEEKEGEDKSQPKSIRERRRPREKRRSTGVSFWTQDSDENEQEQQSDTEEGSNKKETQTDSISRYETSSTSAGDRYDSLLGRSGSYSYLEERKPYSSRLEKDDSTDFKKLYEQILAENEKLKAQLHDTNMELTDLKLQLEKATQRQERFADRSLLEMEKRERRALERRISEMEEELKMLPDLKADNQRLKDENGALIRVISKLSK

PTMs - O14974 As Substrate

O14974

Site	PTM Type	Enzyme	Source
K9	Acetylation		Uniprot
S20	Phosphorylation		Uniprot
T22	Phosphorylation		Uniprot
T34	Phosphorylation		Uniprot
Y68	Phosphorylation		Uniprot
T234	Phosphorylation		Uniprot
K244	Ubiquitination		Uniprot
K285	Ubiquitination		Uniprot
K286	Ubiquitination		Uniprot
S292	Phosphorylation		Uniprot
S299	Phosphorylation		Uniprot
S304	Phosphorylation		Uniprot
S313	Phosphorylation		Uniprot
S336	Phosphorylation		Uniprot
S365	Phosphorylation		Uniprot
T406	Phosphorylation		Uniprot
T408	Phosphorylation		Uniprot
S409	Phosphorylation		Uniprot
T416	Phosphorylation		Uniprot
T417	Phosphorylation		Uniprot
T419	Phosphorylation		Uniprot
S422	Phosphorylation		Uniprot
S432	Phosphorylation	P06493 (CDK1)	Uniprot
K442	Acetylation		Uniprot
T443	Phosphorylation	O75116 (ROCK2)	Uniprot
S445	Phosphorylation	O60285 (NUAK1) , O95835 (LATS1)	Uniprot
Y446	Phosphorylation		Uniprot
T453	Phosphorylation		Uniprot
K456	Acetylation		Uniprot
K460	Acetylation		Uniprot
T464	Phosphorylation		Uniprot
S470	Phosphorylation		Uniprot
S472	Phosphorylation	O60285 (NUAK1)	Uniprot
S473	Phosphorylation	P06493 (CDK1)	Uniprot
S477	Phosphorylation		Uniprot
S478	Phosphorylation		Uniprot
S479	Phosphorylation		Uniprot
S489	Phosphorylation		Uniprot
Y496	Phosphorylation		Uniprot
T500	Phosphorylation		Uniprot
S507	Phosphorylation		Uniprot
T508	Phosphorylation		Uniprot
S509	Phosphorylation		Uniprot
S520	Phosphorylation		Uniprot
S525	Phosphorylation		Uniprot
T529	Phosphorylation		Uniprot
K539	Methylation		Uniprot
S541	Phosphorylation		Uniprot
S542	Phosphorylation		Uniprot
S547	Phosphorylation		Uniprot
T548	Phosphorylation		Uniprot
Y549	Phosphorylation		Uniprot
K551	Methylation		Uniprot
S552	Phosphorylation		Uniprot
S554	Phosphorylation		Uniprot
T571	Phosphorylation		Uniprot
T575	Phosphorylation		Uniprot
S578	Phosphorylation		Uniprot
S585	Phosphorylation		Uniprot
S601	Phosphorylation	P06493 (CDK1)	Uniprot
S607	Phosphorylation		Uniprot
S608	Phosphorylation		Uniprot
T609	Phosphorylation		Uniprot
S618	Phosphorylation		Uniprot
T631	Phosphorylation		Uniprot
T645	Phosphorylation		Uniprot
T647	Phosphorylation		Uniprot
T652	Phosphorylation		Uniprot
S668	Phosphorylation		Uniprot
Y669	Phosphorylation		Uniprot
T671	Phosphorylation		Uniprot
S678	Phosphorylation		Uniprot
S680	Phosphorylation		Uniprot
S692	Phosphorylation	Q13976 (PRKG1)	Uniprot
S695	Phosphorylation	P17612 (PRKACA) , Q13976 (PRKG1)	Uniprot
T696	Phosphorylation	Q09013 (DMPK) , P04049 (RAF1) , Q13464 (ROCK1) , P17612 (PRKACA) , O75116 (ROCK2) , Q13418 (ILK)	Uniprot
T700	Phosphorylation		Uniprot
T702	Phosphorylation		Uniprot
T710	Phosphorylation	Q13418 (ILK)	Uniprot
S716	Phosphorylation		Uniprot
T717	Phosphorylation		Uniprot
T719	Phosphorylation		Uniprot
K743	Acetylation		Uniprot
T748	Phosphorylation		Uniprot
S749	Phosphorylation		Uniprot
Y754	Phosphorylation		Uniprot
K755	Acetylation		Uniprot
Y758	Phosphorylation		Uniprot
S759	Phosphorylation		Uniprot
T761	Phosphorylation		Uniprot
Y762	Phosphorylation		Uniprot
T765	Phosphorylation		Uniprot
Y766	Phosphorylation		Uniprot
Y769	Phosphorylation		Uniprot
S781	Phosphorylation		Uniprot
S790	Phosphorylation		Uniprot
Y792	Phosphorylation		Uniprot
S794	Phosphorylation		Uniprot
S795	Phosphorylation		Uniprot
S802	Phosphorylation		Uniprot
Y810	Phosphorylation		Uniprot
S811	Phosphorylation		Uniprot
S839	Phosphorylation		Uniprot
S852	Phosphorylation	P17612 (PRKACA) , O75116 (ROCK2) , Q13976 (PRKG1) , Q9Y5S2 (CDC42BPB) , Q13464 (ROCK1)	Uniprot
T853	Phosphorylation	P17612 (PRKACA) , Q13464 (ROCK1) , Q04759 (PRKCQ)	Uniprot
S856	Phosphorylation		Uniprot
T859	Phosphorylation		Uniprot
S862	Phosphorylation		Uniprot
S871	Phosphorylation		Uniprot
T873	Phosphorylation		Uniprot
S877	Phosphorylation		Uniprot
T884	Phosphorylation		Uniprot
S886	Phosphorylation		Uniprot
S888	Phosphorylation		Uniprot
Y890	Phosphorylation		Uniprot
T892	Phosphorylation		Uniprot
S893	Phosphorylation		Uniprot
S894	Phosphorylation		Uniprot
S896	Phosphorylation		Uniprot
Y901	Phosphorylation		Uniprot
S903	Phosphorylation		Uniprot
S908	Phosphorylation		Uniprot
S910	Phosphorylation	O60285 (NUAK1)	Uniprot
Y911	Phosphorylation		Uniprot
S912	Phosphorylation		Uniprot
Y913	Phosphorylation		Uniprot
Y920	Phosphorylation		Uniprot
S921	Phosphorylation		Uniprot
S922	Phosphorylation		Uniprot
Y936	Phosphorylation		Uniprot
K945	Ubiquitination		Uniprot
S978	Phosphorylation		Uniprot
S995	Phosphorylation		Uniprot
K1002	Methylation		Uniprot
K1008	Methylation		Uniprot
S1029	Phosphorylation		Uniprot

Research Backgrounds

O14974_MYPT1_HUMAN

Function:

Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.

PTMs:

Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR. In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1. Phosphorylation at Ser-445, Ser-472 and Ser-910 by NUAK1 promotes interaction with 14-3-3, leading to inhibit interaction with myosin light chain MLC2, preventing dephosphorylation of MLC2. May be phosphorylated at Thr-696 by DMPK; may inhibit the myosin phosphatase activity. Phosphorylated at Ser-473 by CDK1 during mitosis, creating docking sites for the POLO box domains of PLK1. Subsequently, PLK1 binds and phosphorylates PPP1R12A.

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton>Stress fiber.
Note: Also along actomyosin filaments.

Tissue Specificity:

Expressed in striated muscles, specifically in type 2a fibers (at protein level).

Subunit Structure:

PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12A mediates binding to myosin. Interacts with ARHA and CIT (By similarity). Binds PPP1R12B, ROCK1 and IL16. Interacts directly with PRKG1. Non-covalent dimer of 2 dimers; PRKG1-PRKG1 and PPP1R12A-PPP1R12A. Interacts with SMTNL1 (By similarity). Interacts with PPP1CB; the interaction is direct. Interacts (when phosphorylated at Ser-445, Ser-472 and Ser-910) with 14-3-3. Interacts with ROCK1 and ROCK2. Interacts with isoform 1 and isoform 2 of ZIPK/DAPK3. Interacts with RAF1. Interacts with HIF1AN. Interacts with NCKAP1L.

Family&Domains:

Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.

The KVKF motif mediates interaction with PPP1CB.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)

· Organismal Systems > Immune system > Platelet activation. (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

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Phospho-MYPT1 (Ser473) Antibody - #AF3776