Phospho-TIF1 alpha (Ser649) Antibody

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Western blot analysis of extracts from Hela cells(serum starvation treatment), using Phospho-TIF1 alpha (Ser649) Antibody.

Product:	Phospho-TIF1 alpha (Ser649) Antibody
Catalog:	AF3774
Description:	Rabbit polyclonal antibody to Phospho-TIF1 alpha (Ser649)
Application:	WB
Reactivity:	Human
Mol.Wt.:	74kD(Calculated).
Uniprot:	Q9NYV6
RRID:	AB_2847088

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

Phospho-TIF1 alpha (Ser649) Antibody detects endogenous levels of TIF1 alpha only when phosphorylated at Ser649.

RRID:

AB_2847088
Cite Format: Affinity Biosciences Cat# AF3774, RRID:AB_2847088.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AL023001; DKFZp566E104; E130302O19Rik; MGC104238; R75565; RGD1305001; RNA polymerase I-specific transcription initiation factor RRN3; Rrn3; RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae); RRN3_HUMAN; TIF-IA; TIF1A; TIFIA; Transcription Initiation Factor 1A; Transcription initiation factor IA; Transcription initiation factor TIF IA;

Immunogens

Immunogen:

A synthesized peptide derived from human TIF1 alpha around the phosphorylation site of Ser649.

Uniprot:

Q9NYV6(RRN3_HUMAN) >>Visit HPA database.

Gene(ID):

RRN3(54700)

Sequence:

Q9NYV6 RRN3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAPLLHTRLPGDAAASSSAVKKLGASRTGISNMRALENDFFNSPPRKTVRFGGTVTEVLLKYKKGETNDFELLKNQLLDPDIKDDQIINWLLEFRSSIMYLTKDFEQLISIILRLPWLNRSQTVVEEYLAFLGNLVSAQTVFLRPCLSMIASHFVPPRVIIKEGDVDVSDSDDEDDNLPANFDTCHRALQIIARYVPSTPWFLMPILVEKFPFVRKSERTLECYVHNLLRISVYFPTLRHEILELIIEKLLKLDVNASRQGIEDAEETATQTCGGTDSTEGLFNMDEDEETEHETKAGPERLDQMVHPVAERLDILMSLVLSYMKDVCYVDGKVDNGKTKDLYRDLINIFDKLLLPTHASCHVQFFMFYLCSFKLGFAEAFLEHLWKKLQDPSNPAIIRQAAGNYIGSFLARAKFIPLITVKSCLDLLVNWLHIYLNNQDSGTKAFCDVALHGPFYSACQAVFYTFVFRHKQLLSGNLKEGLQYLQSLNFERIVMSQLNPLKICLPSVVNFFAAITNKYQLVFCYTIIERNNRQMLPVIRSTAGGDSVQICTNPLDTFFPFDPCVLKRSKKFIDPIYQVWEDMSAEELQEFKKPMKKDIVEDEDDDFLKGEVPQNDTVIGITPSSFDTHFRSPSSSVGSPPVLYMQPSPL

PTMs - Q9NYV6 As Substrate

Q9NYV6

Site	PTM Type	Enzyme	Source
R9	Methylation		Uniprot
S19	Phosphorylation		Uniprot
K22	Ubiquitination		Uniprot
S44	Phosphorylation	P24941 (CDK2)	Uniprot
K62	Ubiquitination		Uniprot
K65	Ubiquitination		Uniprot
K75	Ubiquitination		Uniprot
S170	Phosphorylation	P68400 (CSNK2A1)	Uniprot
S172	Phosphorylation	P68400 (CSNK2A1)	Uniprot
S199	Phosphorylation		Uniprot
T200	Phosphorylation	P45984 (MAPK9)	Uniprot
S233	Phosphorylation		Uniprot
K253	Ubiquitination		Uniprot
T271	Phosphorylation		Uniprot
S319	Phosphorylation		Uniprot
K334	Ubiquitination		Uniprot
K415	Ubiquitination		Uniprot
K480	Ubiquitination		Uniprot
K568	Ubiquitination		Uniprot
K593	Ubiquitination		Uniprot
K598	Ubiquitination		Uniprot
K610	Ubiquitination		Uniprot
T623	Phosphorylation		Uniprot
S633	Phosphorylation	P27361 (MAPK3)	Uniprot
S635	Phosphorylation	Q13131 (PRKAA1)	Uniprot
S636	Phosphorylation		Uniprot
S640	Phosphorylation		Uniprot
S649	Phosphorylation	P51812 (RPS6KA3) , P27361 (MAPK3)	Uniprot

Research Backgrounds

Q9NYV6_RRN3_HUMAN

Function:

Required for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent preinitiation complex (PIC). Dissociates from pol I as a consequence of transcription. In vitro, cannot activate transcription in a subsequent transcription reaction (By similarity).

PTMs:

Phosphorylation is required for participation in rDNA transcription (By similarity). Phosphorylated at Thr-200 by MAPK9/JNK2, which abrogates initiation complex formation.

Subcellular Location:

Nucleus>Nucleolus.

Subunit Structure:

Interacts with TWISTNB, EIF3L, TAF1B and TAF1C.

Family&Domains:

Belongs to the RRN3 family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Phospho-TIF1 alpha (Ser649) Antibody - #AF3774