Phospho-SLC31A1 / CTR1 (Thr114) Antibody

Product:	Phospho-SLC31A1 / CTR1 (Thr114) Antibody
Catalog:	AF3773
Description:	Rabbit polyclonal antibody to Phospho-SLC31A1 / CTR1 (Thr114)
Application:	ELISA(peptide)
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	21kD(Calculated).
Uniprot:	O15431
RRID:	AB_2847087

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Phospho-SLC31A1 / CTR1 (Thr114) Antibody detects endogenous levels of SLC31A1 / CTR1 only when phosphorylated at Thr114.

RRID:

AB_2847087
Cite Format: Affinity Biosciences Cat# AF3773, RRID:AB_2847087.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Copper transport 1 homolog; Copper transporter 1; COPT1; COPT1_HUMAN; CTR1; hCTR1; High affinity copper uptake protein 1; SLC31A1; solute carrier family 31 (copper tansporters) member 1; Solute carrier family 31 member 1;

Immunogens

Immunogen:

A synthesized peptide derived from human SLC31A1 / CTR1 around the phosphorylation site of Thr114.

Uniprot:

O15431(COPT1_HUMAN) >>Visit HPA database.

Gene(ID):

SLC31A1(1317)

Sequence:

O15431 COPT1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDHSHHMGMSYMDSNSTMQPSHHHPTTSASHSHGGGDSSMMMMPMTFYFGFKNVELLFSGLVINTAGEMAGAFVAVFLLAMFYEGLKIARESLLRKSQVSIRYNSMPVPGPNGTILMETHKTVGQQMLSFPHLLQTVLHIIQVVISYFLMLIFMTYNGYLCIAVAAGAGTGYFLFSWKKAVVVDITEHCH

PTMs - O15431 As Substrate

O15431

Site	PTM Type	Source
S4	Phosphorylation	Uniprot
S10	Phosphorylation	Uniprot
Y11	Phosphorylation	Uniprot
N15	N-Glycosylation	Uniprot
T27	O-Glycosylation	Uniprot
K96	Ubiquitination	Uniprot
S105	Phosphorylation	Uniprot
T114	Phosphorylation	Uniprot

Research Backgrounds

O15431_COPT1_HUMAN

Function:

High-affinity, saturable copper transporter involved in dietary copper uptake.

PTMs:

O-Glycosylation at Thr-27 protects from proteolytic cleavage in the N-terminal extracellular domain.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.
Note: Localizes to the apical membrane in intestinal epithelial cells.

Subunit Structure:

Homotrimer.

Family&Domains:

Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.

Research Fields

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Organismal Systems > Digestive system > Mineral absorption.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Phospho-SLC31A1 / CTR1 (Thr114) Antibody - #AF3773