Phospho-XIRP1 (Ser295) Antibody | Affinity Biosciences

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Western blot analysis of extracts from Rat heart, using Phospho-XIRP1 (Ser295) Antibody.

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Product:	Phospho-XIRP1 (Ser295) Antibody
Catalog:	AF3762
Description:	Rabbit polyclonal antibody to Phospho-XIRP1 (Ser295)
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	110kDa; 123kD(Calculated).
Uniprot:	O70373
RRID:	AB_2847076

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Phospho-XIRP1 (Ser295) Antibody detects endogenous levels of XIRP1 only when phosphorylated at Ser295.

RRID:

AB_2847076
Cite Format: Affinity Biosciences Cat# AF3762, RRID:AB_2847076.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:

A synthesized peptide derived from mouse XIRP1 around the phosphorylation site of Ser295.

Uniprot:

O70373(XIRP1_MOUSE) >>Visit HPA database.

Expression:

O70373 XIRP1_MOUSE:

Expressed in heart and skeletal muscle. In heart, present in intercalated disks (at protein level).

Sequence:

O70373 XIRP1_MOUSE:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MADAQMQVAPTPTIQMRTEEDLSLPHPSAPEGLPPPPPKETFSKFQQQRQASELRRLYKHIHPELRKNLEEAVAEDLAEVLGSEEPTEGDVQCMRWIFENWRLDAIGDHERPAAREPVSGGNVQATSRKFEEGSFTNSSDQEPEGLRPSGGDVQAARQMFETKPLDALRGQEEATQTTMREPAATGDVQGTRKLFETRPLDRLGSRPSIQEQSPLELRSEIQELKGDVKKTVKLFQTEPLCAIQDAEGTIHEVKAACREEIQSNAVRSARWLFETRPLDAFNQDPSQVRVIRGISLEEGALPDVSATRWIFETQPLDAIREIEVDEKDFQPSPDLIPPGPDVQHQRHLFETCSLDTLKGERETEAEVPPKEEVIPGDVRSTLWLFETKPLDAFRDQVQVGHLQRVGHQEGEGLVTECLPSNGTSVLPLSQGVPQNDGLKGDVKTFKNLFETLPLDSIGQGEPSAYGNINRGQNTDSAEQSQGSDAPVYAMQDSRGQLHALTSVSREQVVGGDVQGYKWMFETQPLDTLGRSPSTIDVVRGITRQEVVAGDVGTTRWLFETQPLEMIHQQEQQKPEEEEGKGPGGPPPELPKKGDVQTIRWLFETYPMSELAEKRESEVTDPVSKAETQSCTWMFGPQSLNPAEGSGEQHLQTSQVPAGDRQTDRHVFETESLPASNQSSGRKPVRYCSRVEIPSGQVSRQKEVFQALEAGKKEVPETTINLGSIPTGSVHKFTWLFENCPMGSLAAESIRGDNLQEEQPKGSAGHGTPERQETAAERTLRTLHATPGILHHGGILMEARGPGELCLAKYVLPSPGQGRPYIRKEELVCGELPRIVRQVLRRTDVDQQGLLVQEDTAGQLQLHPLMLPGPGDPGNIEDMDPELQQLLACGLGVSVSKTGLVMQETGQGLVALTAYSLQPQLTSRAPERSSVQLLASCIDKGDLHSLHSLRWEPPTDPSSGPATEESQRVPPTESIIHVTPLDSTMEMGQLRISGSTPCPPPSRAAGKVVLPNGKPVAQAPLQEARKKRDISHAGQKGKAASGRPEASPLGSGAPDLQEAMQNLRLATAEAQSLHQQVLSRHPQGSDPVATSMPVQDVLQASTPATGVTQGSIRPVAGSEARIPAVPRKLL

Research Backgrounds

O70373_XIRP1_MOUSE

Function:

Protects actin filaments from depolymerization.

Subcellular Location:

Cell junction.
Note: Colocalizes with actin stress fibers.

Tissue Specificity:

Expressed in heart and skeletal muscle. In heart, present in intercalated disks (at protein level).

Subunit Structure:

Interacts with FLNC and VASP (By similarity). Interacts with F-actin and CTNNB1.

Family&Domains:

Xin repeats bind F-actin.

Belongs to the Xin family.

Restrictive clause

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Phospho-XIRP1 (Ser295) Antibody - #AF3762