Product: Phospho-EIF3J (Ser127) Antibody
Catalog: AF3761
Description: Rabbit polyclonal antibody to Phospho-EIF3J (Ser127)
Application: IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 29kD(Calculated).
Uniprot: O75822
RRID: AB_2847075

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 100ul $350 In stock
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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-EIF3J (Ser127) Antibody detects endogenous levels of EIF3J only when phosphorylated at Ser127.
RRID:
AB_2847075
Cite Format: Affinity Biosciences Cat# AF3761, RRID:AB_2847075.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

eIF 3 alpha; eIF-3-alpha; eIF3 alpha; eIF3 p35; eIF3j; EIF3J_HUMAN; EIF3S 1; EIF3S1; Eukaryotic translation initiation factor 3 subunit 1; Eukaryotic translation initiation factor 3 subunit J; Eukaryotic translation initiation factor 3, subunit 1 (alpha, 35kD); Eukaryotic translation initiation factor 3, subunit 1 alpha, 35kD;

Immunogens

Immunogen:

A synthesized peptide derived from human EIF3J around the phosphorylation site of Ser127.

Uniprot:
Gene(ID):
Sequence:
MAAAAAAAGDSDSWDADAFSVEDPVRKVGGGGTAGGDRWEGEDEDEDVKDNWDDDDDEKKEEAEVKPEVKISEKKKIAEKIKEKERQQKKRQEEIKKRLEEPEEPKVLTPEEQLADKLRLKKLQEESDLELAKETFGVNNAVYGIDAMNPSSRDDFTEFGKLLKDKITQYEKSLYYASFLEVLVRDVCISLEIDDLKKITNSLTVLCSEKQKQEKQSKAKKKKKGVVPGGGLKATMKDDLADYGGYDGGYVQDYEDFM

PTMs - O75822 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S11 Phosphorylation
S13 Phosphorylation
S20 Phosphorylation
K27 Ubiquitination
T33 Phosphorylation
R38 Methylation
S72 Phosphorylation
T109 Phosphorylation
K117 Acetylation
K117 Ubiquitination
K122 Ubiquitination
S127 Phosphorylation P68400 (CSNK2A1)
K133 Ubiquitination
Y143 Phosphorylation
T157 Phosphorylation
K161 Ubiquitination
Y175 Phosphorylation
K197 Acetylation
C207 S-Nitrosylation
S208 Phosphorylation
K220 Acetylation
K221 Acetylation
K222 Acetylation
K223 Acetylation
K224 Ubiquitination
K233 Methylation
K233 Ubiquitination
T235 Phosphorylation
K237 Methylation
Y243 Phosphorylation
Y246 Phosphorylation
Y250 Phosphorylation
Y254 Phosphorylation

Research Backgrounds

Function:

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.

PTMs:

Phosphorylated. Phosphorylation is enhanced upon serum stimulation.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.

Family&Domains:

Belongs to the eIF-3 subunit J family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

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