Product: Phospho-LETM1 (Thr192) Antibody
Catalog: AF3759
Description: Rabbit polyclonal antibody to Phospho-LETM1 (Thr192)
Application: WB
Reactivity: Human, Mouse
Mol.Wt.: 83kD(Calculated).
Uniprot: O95202
RRID: AB_2847073

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 100ul $350 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
Phospho-LETM1 (Thr192) Antibody detects endogenous levels of LETM1 only when phosphorylated at Thr192.
RRID:
AB_2847073
Cite Format: Affinity Biosciences Cat# AF3759, RRID:AB_2847073.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

LETM 1; LETM1 and EF hand domain containing protein 1, mitochondrial; LETM1 and EF-hand domain-containing protein 1; Letm1; LETM1_HUMAN; Leucine zipper EF hand containing transmembrane protein 1; Leucine zipper-EF-hand-containing transmembrane protein 1; Mdm38 homolog; mitochondrial;

Immunogens

Immunogen:

A synthesized peptide derived from human LETM1 around the phosphorylation site of Thr192.

Uniprot:
Gene(ID):
Sequence:
MASILLRSCRGRAPARLPPPPRYTVPRGSPGDPAHLSCASTLGLRNCLNVPFGCCTPIHPVYTSSRGDHLGCWALRPECLRIVSRAPWTSTSVGFVAVGPQCLPVRGWHSSRPVRDDSVVEKSLKSLKDKNKKLEEGGPVYSPPAEVVVKKSLGQRVLDELKHYYHGFRLLWIDTKIAARMLWRILNGHSLTRRERRQFLRICADLFRLVPFLVFVVVPFMEFLLPVAVKLFPNMLPSTFETQSLKEERLKKELRVKLELAKFLQDTIEEMALKNKAAKGSATKDFSVFFQKIRETGERPSNEEIMRFSKLFEDELTLDNLTRPQLVALCKLLELQSIGTNNFLRFQLTMRLRSIKADDKLIAEEGVDSLNVKELQAACRARGMRALGVTEDRLRGQLKQWLDLHLHQEIPTSLLILSRAMYLPDTLSPADQLKSTLQTLPEIVAKEAQVKVAEVEGEQVDNKAKLEATLQEEAAIQQEHREKELQKRSEVAKDFEPERVVAAPQRPGTEPQPEMPDTVLQSETLKDTAPVLEGLKEEEITKEEIDILSDACSKLQEQKKSLTKEKEELELLKEDVQDYSEDLQEIKKELSKTGEEKYVEESKASKRLTKRVQQMIGQIDGLISQLEMDQQAGKLAPANGMPTGENVISVAELINAMKQVKHIPESKLTSLAAALDENKDGKVNIDDLVKVIELVDKEDVHISTSQVAEIVATLEKEEKVEEKEKAKEKAEKEVAEVKS

PTMs - O95202 As Substrate

Site PTM Type Enzyme
S3 Phosphorylation
S8 Phosphorylation
S126 Phosphorylation
Y141 Phosphorylation
S142 Phosphorylation
Y164 Phosphorylation
T175 Phosphorylation
S190 Phosphorylation
T192 Phosphorylation
K252 Ubiquitination
K257 Ubiquitination
K284 Acetylation
S287 Phosphorylation
K292 Ubiquitination
R307 Methylation
K373 Ubiquitination
R393 Methylation
Y579 Phosphorylation
S580 Phosphorylation
K597 Acetylation
S602 Phosphorylation
T643 Phosphorylation
S649 Phosphorylation
S666 Phosphorylation
K682 Ubiquitination
S739 Phosphorylation

Research Backgrounds

Function:

Mitochondrial proton/calcium antiporter that mediates proton-dependent calcium efflux from mitochondrion. Crucial for the maintenance of mitochondrial tubular networks and for the assembly of the supercomplexes of the respiratory chain. Required for the maintenance of the tubular shape and cristae organization. In contrast to SLC8B1/NCLX, does not constitute the major factor for mitochondrial calcium extrusion.

Subcellular Location:

Mitochondrion inner membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homohexamer (By similarity). Can form 2 complexes: a major (300 kDa) and a minor complex (500-600 kDa). Interacts with BCS1L.

Family&Domains:

Belongs to the LETM1 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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