Phospho-GAP43 (Ser41) Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from COS-7 cells(uv treatment), using Phospho-GAP43 (Ser41) Antibody.

AF3715 staining Hela cells(heat shock treatment) by IF/ICC.

Product:	Phospho-GAP43 (Ser41) Antibody
Catalog:	AF3715
Description:	Rabbit polyclonal antibody to Phospho-GAP43 (Ser41)
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat, Monkey
Mol.Wt.:	25kD(Calculated).
Uniprot:	P17677
RRID:	AB_2847029

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Monkey

Clonality:

Polyclonal

Specificity:

Phospho-GAP43 (Ser41) Antibody detects endogenous levels of GAP43 only when phosphorylated at Ser41.

RRID:

AB_2847029
Cite Format: Affinity Biosciences Cat# AF3715, RRID:AB_2847029.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Axonal membrane protein GAP 43; Axonal membrane protein GAP-43; B 50; Calmodulin binding protein P 57; F1; GAP 43; GAP43; Growth Associated Protein 43; Growth-associated protein 43; Nerve Growth Related Peptide; Nerve growth related peptide GAP43; NEUM_HUMAN; Neural phosphoprotein B 50; Neural phosphoprotein B-50; Neuromodulin; Neuron growth associated protein 43; PP46; Protein F1; QtrA-11580; QtrA-13071;

Immunogens

Immunogen:

A synthesized peptide derived from human GAP43 around the phosphorylation site of Ser41.

Uniprot:

P17677(NEUM_HUMAN) >>Visit HPA database.

Gene(ID):

GAP43(2596)

Sequence:

P17677 NEUM_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLCCMRRTKQVEKNDDDQKIEQDGIKPEDKAHKAATKIQASFRGHITRKKLKGEKKDDVQAAEAEANKKDEAPVADGVEKKGEGTTTAEAAPATGSKPDEPGKAGETPSEEKKGEGDAATEQAAPQAPASSEEKAGSAETESATKASTDNSPSSKAEDAPAKEEPKQADVPAAVTAAAATTPAAEDAAAKATAQPPTETGESSQAEENIEAVDETKPKESARQDEGKEEEPEADQEHA

PTMs - P17677 As Substrate

P17677

Site	PTM Type	Enzyme	Source
T36	Phosphorylation		Uniprot
K37	Ubiquitination		Uniprot
S41	Phosphorylation	Q02156 (PRKCE)	Uniprot
T107	Phosphorylation		Uniprot
S109	Phosphorylation		Uniprot
S131	Phosphorylation		Uniprot
S142	Phosphorylation		Uniprot
T144	Phosphorylation		Uniprot
S147	Phosphorylation		Uniprot
T148	Phosphorylation		Uniprot
S151	Phosphorylation		Uniprot
S154	Phosphorylation		Uniprot
T181	Phosphorylation		Uniprot
K216	Methylation		Uniprot

Research Backgrounds

P17677_NEUM_HUMAN

Function:

This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.

PTMs:

Phosphorylated (By similarity). Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity (By similarity).

Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2.

Subcellular Location:

Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Growth cone membrane>Peripheral membrane protein>Cytoplasmic side. Cell junction>Synapse. Cell projection>Filopodium membrane>Peripheral membrane protein. Perikaryon. Cell projection>Dendrite. Cell projection>Axon. Cytoplasm.
Note: Cytoplasmic surface of growth cone and synaptic plasma membranes.

Subunit Structure:

Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN (By similarity). Interacts (via IQ domain) with calmodulin (By similarity). Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence (By similarity).

Family&Domains:

Belongs to the neuromodulin family.

Restrictive clause

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Phospho-GAP43 (Ser41) Antibody - #AF3715