Phospho-Rab3A (Thr86) Antibody - #AF3707

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Product: Phospho-Rab3A (Thr86) Antibody
Catalog: AF3707
Description: Rabbit polyclonal antibody to Phospho-Rab3A (Thr86)
Application: IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 25kD(Calculated).
Uniprot: P20336
RRID: AB_2847021

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-Rab3A (Thr86) Antibody detects endogenous levels of Rab3A only when phosphorylated at Thr86.
RRID:
AB_2847021
Cite Format: Affinity Biosciences Cat# AF3707, RRID:AB_2847021.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Rab 3A; RAB 3A member RAS oncogene family; Rab3a; RAB3A member RAS oncogene family; RAB3A_HUMAN; RAS associated protein RAB 3A; RAS associated protein RAB3A; Ras related protein Rab 3A; Ras related protein Rab3A; Ras-related protein Rab-3A;

Immunogens

Immunogen:

A synthesized peptide derived from human Rab3A around the phosphorylation site of Thr86.

Uniprot:

P20336(RAB3A_HUMAN) >>Visit HPA database.

Gene(ID):
RAB3A(5864)
Expression:
P20336 RAB3A_HUMAN:

Specifically expressed in brain.

Sequence:
MASATDSRYGQKESSDQNFDYMFKILIIGNSSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTIYRNDKRIKLQIWDTAGQERYRTITTAYYRGAMGFILMYDITNEESFNAVQDWSTQIKTYSWDNAQVLLVGNKCDMEDERVVSSERGRQLADHLGFEFFEASAKDNINVKQTFERLVDVICEKMSESLDTADPAVTGAKQGPQLSDQQVPPHQDCAC

PTMs - P20336 As Substrate

Site PTM Type Enzyme
Y65 Phosphorylation
T78 Phosphorylation
T88 Phosphorylation
Y91 Phosphorylation
K173 Ubiquitination
S190 Phosphorylation

Research Backgrounds

Function:

Small GTP-binding protein that plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane (By similarity). Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane (By similarity). Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed (By similarity). Stimulates insulin secretion through interaction with RIMS2 or RPH3AL effectors in pancreatic beta cells (By similarity). Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9. Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1. Plays also a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT (By similarity).

PTMs:

Phosphorylation of Thr-86 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2.

Subcellular Location:

Cytoplasm>Cytosol. Lysosome. Cytoplasmic vesicle>Secretory vesicle. Cell membrane>Lipid-anchor>Cytoplasmic side.
Note: Cycles between a vesicle-associated GTP-bound form and a cytosolic GDP-bound form.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Specifically expressed in brain.

Subunit Structure:

Interacts with RIMS1 and RIMS2 (By similarity). Interacts with Rabphilin-3A/RPH3A and Rab effector Noc2/RPH3AL (By similarity). Interacts with SYTL4 (By similarity). Interacts with RAB3IP (By similarity). Interacts with SGSM1 and SGSM3 (By similarity). Interacts with SYT1 (By similarity). Interacts with MYH9; this interaction is essential for lysosome exocytosis and plasma membrane repair. Interacts with STXBP1; this interaction promotes RAB3A dissociation from the vesicle membrane (By similarity). Interacts with SNCA. Interacts with GDI1, GDI2, CHM and CHML; phosphorylation at Thr-86 disrupts these interactions.

Family&Domains:

Belongs to the small GTPase superfamily. Rab family.

Research Fields

· Organismal Systems > Nervous system > Synaptic vesicle cycle.

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

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