Phospho-M6PR (Ser2409) Antibody | Affinity Biosciences

Product:	Phospho-M6PR (Ser2409) Antibody
Catalog:	AF3705
Description:	Rabbit polyclonal antibody to Phospho-M6PR (Ser2409)
Application:	ELISA(peptide)
Reactivity:	Human, Mouse
Mol.Wt.:	274kD(Calculated).
Uniprot:	P11717
RRID:	AB_2847019

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

Phospho-M6PR (Ser2409) Antibody detects endogenous levels of M6PR only when phosphorylated at Ser2409.

RRID:

AB_2847019
Cite Format: Affinity Biosciences Cat# AF3705, RRID:AB_2847019.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

300 kDa mannose 6 phosphate receptor; 300 kDa mannose 6-phosphate receptor; Cation independent mannose 6 phosphate receptor; Cation-independent mannose-6-phosphate receptor; CD222; CD222 antigen; CI Man 6 P receptor; CI Man-6-P receptor; CI MPR; CI-M6PR; CI-MPR; CIMPR; IGF 2 receptor; IGF 2R; IGF II receptor; IGF-II receptor; IGF2 receptor; Igf2r; Insulin like growth factor 2 receptor; Insulin like growth factor II receptor; Insulin-like growth factor 2 receptor; Insulin-like growth factor II receptor; M6P R; M6P/IGF2 receptor; M6P/IGF2R; M6PR; mannose 6 phosphate receptor; mannose 6 phosphate receptor, cation independent; MPR 300; MPR300; MPRI; MPRI_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human M6PR around the phosphorylation site of Ser2409.

Uniprot:

P11717(MPRI_HUMAN) >>Visit HPA database.

Gene(ID):

IGF2R(3482)

Sequence:

P11717 MPRI_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGAAAGRSPHLGPAPARRPQRSLLLLQLLLLVAAPGSTQAQAAPFPELCSYTWEAVDTKNNVLYKINICGSVDIVQCGPSSAVCMHDLKTRTYHSVGDSVLRSATRSLLEFNTTVSCDQQGTNHRVQSSIAFLCGKTLGTPEFVTATECVHYFEWRTTAACKKDIFKANKEVPCYVFDEELRKHDLNPLIKLSGAYLVDDSDPDTSLFINVCRDIDTLRDPGSQLRACPPGTAACLVRGHQAFDVGQPRDGLKLVRKDRLVLSYVREEAGKLDFCDGHSPAVTITFVCPSERREGTIPKLTAKSNCRYEIEWITEYACHRDYLESKTCSLSGEQQDVSIDLTPLAQSGGSSYISDGKEYLFYLNVCGETEIQFCNKKQAAVCQVKKSDTSQVKAAGRYHNQTLRYSDGDLTLIYFGGDECSSGFQRMSVINFECNKTAGNDGKGTPVFTGEVDCTYFFTWDTEYACVKEKEDLLCGATDGKKRYDLSALVRHAEPEQNWEAVDGSQTETEKKHFFINICHRVLQEGKARGCPEDAAVCAVDKNGSKNLGKFISSPMKEKGNIQLSYSDGDDCGHGKKIKTNITLVCKPGDLESAPVLRTSGEGGCFYEFEWHTAAACVLSKTEGENCTVFDSQAGFSFDLSPLTKKNGAYKVETKKYDFYINVCGPVSVSPCQPDSGACQVAKSDEKTWNLGLSNAKLSYYDGMIQLNYRGGTPYNNERHTPRATLITFLCDRDAGVGFPEYQEEDNSTYNFRWYTSYACPEEPLECVVTDPSTLEQYDLSSLAKSEGGLGGNWYAMDNSGEHVTWRKYYINVCRPLNPVPGCNRYASACQMKYEKDQGSFTEVVSISNLGMAKTGPVVEDSGSLLLEYVNGSACTTSDGRQTTYTTRIHLVCSRGRLNSHPIFSLNWECVVSFLWNTEAACPIQTTTDTDQACSIRDPNSGFVFNLNPLNSSQGYNVSGIGKIFMFNVCGTMPVCGTILGKPASGCEAETQTEELKNWKPARPVGIEKSLQLSTEGFITLTYKGPLSAKGTADAFIVRFVCNDDVYSGPLKFLHQDIDSGQGIRNTYFEFETALACVPSPVDCQVTDLAGNEYDLTGLSTVRKPWTAVDTSVDGRKRTFYLSVCNPLPYIPGCQGSAVGSCLVSEGNSWNLGVVQMSPQAAANGSLSIMYVNGDKCGNQRFSTRITFECAQISGSPAFQLQDGCEYVFIWRTVEACPVVRVEGDNCEVKDPRHGNLYDLKPLGLNDTIVSAGEYTYYFRVCGKLSSDVCPTSDKSKVVSSCQEKREPQGFHKVAGLLTQKLTYENGLLKMNFTGGDTCHKVYQRSTAIFFYCDRGTQRPVFLKETSDCSYLFEWRTQYACPPFDLTECSFKDGAGNSFDLSSLSRYSDNWEAITGTGDPEHYLINVCKSLAPQAGTEPCPPEAAACLLGGSKPVNLGRVRDGPQWRDGIIVLKYVDGDLCPDGIRKKSTTIRFTCSESQVNSRPMFISAVEDCEYTFAWPTATACPMKSNEHDDCQVTNPSTGHLFDLSSLSGRAGFTAAYSEKGLVYMSICGENENCPPGVGACFGQTRISVGKANKRLRYVDQVLQLVYKDGSPCPSKSGLSYKSVISFVCRPEARPTNRPMLISLDKQTCTLFFSWHTPLACEQATECSVRNGSSIVDLSPLIHRTGGYEAYDESEDDASDTNPDFYINICQPLNPMHGVPCPAGAAVCKVPIDGPPIDIGRVAGPPILNPIANEIYLNFESSTPCLADKHFNYTSLIAFHCKRGVSMGTPKLLRTSECDFVFEWETPVVCPDEVRMDGCTLTDEQLLYSFNLSSLSTSTFKVTRDSRTYSVGVCTFAVGPEQGGCKDGGVCLLSGTKGASFGRLQSMKLDYRHQDEAVVLSYVNGDRCPPETDDGVPCVFPFIFNGKSYEECIIESRAKLWCSTTADYDRDHEWGFCRHSNSYRTSSIIFKCDEDEDIGRPQVFSEVRGCDVTFEWKTKVVCPPKKLECKFVQKHKTYDLRLLSSLTGSWSLVHNGVSYYINLCQKIYKGPLGCSERASICRRTTTGDVQVLGLVHTQKLGVIGDKVVVTYSKGYPCGGNKTASSVIELTCTKTVGRPAFKRFDIDSCTYYFSWDSRAACAVKPQEVQMVNGTITNPINGKSFSLGDIYFKLFRASGDMRTNGDNYLYEIQLSSITSSRNPACSGANICQVKPNDQHFSRKVGTSDKTKYYLQDGDLDVVFASSSKCGKDKTKSVSSTIFFHCDPLVEDGIPEFSHETADCQYLFSWYTSAVCPLGVGFDSENPGDDGQMHKGLSERSQAVGAVLSLLLVALTCCLLALLLYKKERRETVISKLTTCCRRSSNVSYKYSKVNKEEETDENETEWLMEEIQLPPPRQGKEGQENGHITTKSVKALSSLHGDDQDSEDEVLTIPEVKVHSGRGAGAESSHPVRNAQSNALQEREDDRVGLVRGEKARKGKSSSAQQKTVSSTKLVSFHDDSDEDLLHI

PTMs - P11717 As Substrate

P11717

Site	PTM Type	Enzyme	Source
R7	Methylation		Uniprot
K89	Ubiquitination		Uniprot
N112	N-Glycosylation		Uniprot
K170	Ubiquitination		Uniprot
K183	Ubiquitination		Uniprot
K253	Ubiquitination		Uniprot
T296	Phosphorylation		Uniprot
T301	Phosphorylation		Uniprot
T507	Phosphorylation		Uniprot
N581	N-Glycosylation		Uniprot
N626	N-Glycosylation		Uniprot
K687	Ubiquitination		Uniprot
Y700	Phosphorylation		Uniprot
Y701	Phosphorylation		Uniprot
Y709	Phosphorylation		Uniprot
R710	Methylation		Uniprot
N747	N-Glycosylation		Uniprot
K808	Ubiquitination		Uniprot
K833	Ubiquitination		Uniprot
K1052	Ubiquitination		Uniprot
K1104	Ubiquitination		Uniprot
T1107	Phosphorylation		Uniprot
T1111	Phosphorylation		Uniprot
K1230	Ubiquitination		Uniprot
N1246	N-Glycosylation		Uniprot
K1264	Ubiquitination		Uniprot
T1272	Phosphorylation		Uniprot
K1277	Sumoylation		Uniprot
S1280	Phosphorylation		Uniprot
S1281	Phosphorylation		Uniprot
K1293	Ubiquitination		Uniprot
K1301	Ubiquitination		Uniprot
T1346	Phosphorylation		Uniprot
S1347	Phosphorylation		Uniprot
Y1592	Phosphorylation		Uniprot
K1601	Ubiquitination		Uniprot
S1771	Phosphorylation		Uniprot
T1774	Phosphorylation		Uniprot
K1776	Ubiquitination		Uniprot
T1828	Phosphorylation		Uniprot
S1831	Phosphorylation		Uniprot
K1862	Ubiquitination		Uniprot
S1865	Phosphorylation		Uniprot
S1871	Phosphorylation		Uniprot
K1873	Ubiquitination		Uniprot
T2002	Phosphorylation		Uniprot
Y2024	Phosphorylation		Uniprot
Y2025	Phosphorylation		Uniprot
T2087	Phosphorylation		Uniprot
Y2154	Phosphorylation		Uniprot
S2189	Phosphorylation		Uniprot
T2209	Phosphorylation		Uniprot
S2210	Phosphorylation		Uniprot
K2338	Ubiquitination		Uniprot
S2346	Phosphorylation		Uniprot
S2347	Phosphorylation	P17612 (PRKACA)	Uniprot
S2350	Phosphorylation		Uniprot
Y2351	Phosphorylation		Uniprot
K2352	Acetylation		Uniprot
K2352	Ubiquitination		Uniprot
Y2353	Phosphorylation		Uniprot
S2354	Phosphorylation		Uniprot
T2362	Phosphorylation		Uniprot
K2383	Ubiquitination		Uniprot
K2394	Ubiquitination		Uniprot
S2395	Phosphorylation		Uniprot
S2400	Phosphorylation		Uniprot
S2401	Phosphorylation		Uniprot
S2409	Phosphorylation	P68400 (CSNK2A1)	Uniprot
T2415	Phosphorylation		Uniprot
K2420	Ubiquitination		Uniprot
R2425	Methylation		Uniprot
K2470	Ubiquitination		Uniprot
K2476	Ubiquitination		Uniprot
S2479	Phosphorylation		Uniprot
S2484	Phosphorylation	P68400 (CSNK2A1)	Uniprot

Research Backgrounds

P11717_MPRI_HUMAN

Function:

Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4.

Subcellular Location:

Lysosome membrane>Single-pass type I membrane protein.
Note: Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface.

Subunit Structure:

Binds HA-I and HA-II plasma membrane adapters (By similarity). Interacts with DPP4; the interaction is direct. Binds GGA1, GGA2 and GGA3.

Family&Domains:

Contains 15 repeating units of approximately 147 AA harboring four disulfide bonds each. The most highly conserved region within the repeat consists of a stretch of 13 AA that contains cysteines at both ends.

Belongs to the MRL1/IGF2R family.

Research Fields

· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

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Phospho-M6PR (Ser2409) Antibody - #AF3705