Phospho-Kv1.3/KCNA3 (Ser520) Antibody

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Western blot analysis of extracts from COLO205 cells, using Phospho-Kv1.

Product:	Phospho-Kv1.3/KCNA3 (Ser520) Antibody
Catalog:	AF3702
Description:	Rabbit polyclonal antibody to Phospho-Kv1.3/KCNA3 (Ser520)
Application:	WB
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	64kD(Calculated).
Uniprot:	P22001
RRID:	AB_2847016

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Phospho-Kv1.3/KCNA3 (Ser520) Antibody detects endogenous levels of Kv1.3/KCNA3 only when phosphorylated at Ser520.

RRID:

AB_2847016
Cite Format: Affinity Biosciences Cat# AF3702, RRID:AB_2847016.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

HGK 5; HGK5; HLK 3; HLK3; HPCN 3; HPCN3; HuKIII; KCNA 3; Kcna3; KCNA3_HUMAN; KV1.3; MK 3; MK3; OTTHUMP00000032397; PCN 3; PCN3; Potassium channel 3; Potassium voltage gated channel shaker related subfamily member 3; Potassium voltage gated channel subfamily A member 3; Potassium voltage-gated channel subfamily A member 3; Type n potassium channel; Voltage gated potassium channel protein Kv1.3; Voltage gated potassium channel subunit Kv1.3; Voltage-gated K(+) channel HuKIII; Voltage-gated potassium channel subunit Kv1.3;

Immunogens

Immunogen:

A synthesized peptide derived from human Kv1.3/KCNA3 around the phosphorylation site of Ser520.

Uniprot:

P22001(KCNA3_HUMAN) >>Visit HPA database.

Gene(ID):

KCNA3(3738)

Sequence:

P22001 KCNA3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDERLSLLRSPPPPSARHRAHPPQRPASSGGAHTLVNHGYAEPAAGRELPPDMTVVPGDHLLEPEVADGGGAPPQGGCGGGGCDRYEPLPPSLPAAGEQDCCGERVVINISGLRFETQLKTLCQFPETLLGDPKRRMRYFDPLRNEYFFDRNRPSFDAILYYYQSGGRIRRPVNVPIDIFSEEIRFYQLGEEAMEKFREDEGFLREEERPLPRRDFQRQVWLLFEYPESSGPARGIAIVSVLVILISIVIFCLETLPEFRDEKDYPASTSQDSFEAAGNSTSGSRAGASSFSDPFFVVETLCIIWFSFELLVRFFACPSKATFSRNIMNLIDIVAIIPYFITLGTELAERQGNGQQAMSLAILRVIRLVRVFRIFKLSRHSKGLQILGQTLKASMRELGLLIFFLFIGVILFSSAVYFAEADDPTSGFSSIPDAFWWAVVTMTTVGYGDMHPVTIGGKIVGSLCAIAGVLTIALPVPVIVSNFNYFYHRETEGEEQSQYMHVGSCQHLSSSAEELRKARSNSTLSKSEYMVIEEGGMNHSAFPQTPFKTGNSTATCTTNNNPNSCVNIKKIFTDV

PTMs - P22001 As Substrate

P22001

Site	PTM Type	Enzyme	Source
S10	Phosphorylation		Uniprot
K120	Ubiquitination		Uniprot
K134	Ubiquitination		Uniprot
Y161	Phosphorylation	Q16620 (NTRK2)	Uniprot
Y162	Phosphorylation	Q16620 (NTRK2)	Uniprot
Y163	Phosphorylation	Q16620 (NTRK2)	Uniprot
Y187	Phosphorylation	P12931 (SRC)	Uniprot
K196	Ubiquitination		Uniprot
S378	Phosphorylation		Uniprot
Y447	Phosphorylation	P12931 (SRC)	Uniprot
Y499	Phosphorylation	P12931 (SRC) , Q16620 (NTRK2)	Uniprot
S504	Phosphorylation		Uniprot
S511	Phosphorylation		Uniprot
S520	Phosphorylation		Uniprot
T523	Phosphorylation		Uniprot
S525	Phosphorylation		Uniprot
K526	Ubiquitination		Uniprot
S527	Phosphorylation		Uniprot
Y529	Phosphorylation		Uniprot
S564	Phosphorylation		Uniprot
K569	Ubiquitination		Uniprot
K570	Ubiquitination		Uniprot

Research Backgrounds

P22001_KCNA3_HUMAN

Function:

Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

PTMs:

N-glycosylation promotes the cell surface expression.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Subunit Structure:

Heterotetramer of potassium channel proteins. Binds PDZ domains of DLG1, DLG2 and DLG4 (By similarity).

Family&Domains:

The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.3/KCNA3 sub-subfamily.

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Phospho-Kv1.3/KCNA3 (Ser520) Antibody - #AF3702