Product: Phospho-Kv1.3/KCNA3 (Ser520) Antibody
Catalog: AF3702
Description: Rabbit polyclonal antibody to Phospho-Kv1.3/KCNA3 (Ser520)
Application: WB
Reactivity: Human, Mouse, Rat
Mol.Wt.: 64kD(Calculated).
Uniprot: P22001
RRID: AB_2847016

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-Kv1.3/KCNA3 (Ser520) Antibody detects endogenous levels of Kv1.3/KCNA3 only when phosphorylated at Ser520.
RRID:
AB_2847016
Cite Format: Affinity Biosciences Cat# AF3702, RRID:AB_2847016.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HGK 5; HGK5; HLK 3; HLK3; HPCN 3; HPCN3; HuKIII; KCNA 3; Kcna3; KCNA3_HUMAN; KV1.3; MK 3; MK3; OTTHUMP00000032397; PCN 3; PCN3; Potassium channel 3; Potassium voltage gated channel shaker related subfamily member 3; Potassium voltage gated channel subfamily A member 3; Potassium voltage-gated channel subfamily A member 3; Type n potassium channel; Voltage gated potassium channel protein Kv1.3; Voltage gated potassium channel subunit Kv1.3; Voltage-gated K(+) channel HuKIII; Voltage-gated potassium channel subunit Kv1.3;

Immunogens

Immunogen:

A synthesized peptide derived from human Kv1.3/KCNA3 around the phosphorylation site of Ser520.

Uniprot:
Gene(ID):
Sequence:
MDERLSLLRSPPPPSARHRAHPPQRPASSGGAHTLVNHGYAEPAAGRELPPDMTVVPGDHLLEPEVADGGGAPPQGGCGGGGCDRYEPLPPSLPAAGEQDCCGERVVINISGLRFETQLKTLCQFPETLLGDPKRRMRYFDPLRNEYFFDRNRPSFDAILYYYQSGGRIRRPVNVPIDIFSEEIRFYQLGEEAMEKFREDEGFLREEERPLPRRDFQRQVWLLFEYPESSGPARGIAIVSVLVILISIVIFCLETLPEFRDEKDYPASTSQDSFEAAGNSTSGSRAGASSFSDPFFVVETLCIIWFSFELLVRFFACPSKATFSRNIMNLIDIVAIIPYFITLGTELAERQGNGQQAMSLAILRVIRLVRVFRIFKLSRHSKGLQILGQTLKASMRELGLLIFFLFIGVILFSSAVYFAEADDPTSGFSSIPDAFWWAVVTMTTVGYGDMHPVTIGGKIVGSLCAIAGVLTIALPVPVIVSNFNYFYHRETEGEEQSQYMHVGSCQHLSSSAEELRKARSNSTLSKSEYMVIEEGGMNHSAFPQTPFKTGNSTATCTTNNNPNSCVNIKKIFTDV

PTMs - P22001 As Substrate

Site PTM Type Enzyme
S10 Phosphorylation
K120 Ubiquitination
K134 Ubiquitination
Y161 Phosphorylation Q16620 (NTRK2)
Y162 Phosphorylation Q16620 (NTRK2)
Y163 Phosphorylation Q16620 (NTRK2)
Y187 Phosphorylation P12931 (SRC)
K196 Ubiquitination
S378 Phosphorylation
Y447 Phosphorylation P12931 (SRC)
Y499 Phosphorylation P12931 (SRC) , Q16620 (NTRK2)
S504 Phosphorylation
S511 Phosphorylation
S520 Phosphorylation
T523 Phosphorylation
S525 Phosphorylation
K526 Ubiquitination
S527 Phosphorylation
Y529 Phosphorylation
S564 Phosphorylation
K569 Ubiquitination
K570 Ubiquitination

Research Backgrounds

Function:

Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

PTMs:

N-glycosylation promotes the cell surface expression.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterotetramer of potassium channel proteins. Binds PDZ domains of DLG1, DLG2 and DLG4 (By similarity).

Family&Domains:

The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.3/KCNA3 sub-subfamily.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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