Phospho-GRK2 (Ser670) Antibody - #AF3697

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Product: Phospho-GRK2 (Ser670) Antibody
Catalog: AF3697
Description: Rabbit polyclonal antibody to Phospho-GRK2 (Ser670)
Application: WB IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Mol.Wt.: 80kD(Calculated).
Uniprot: P25098
RRID: AB_2847011

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MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-GRK2 (Ser670) Antibody detects endogenous levels of GRK2 only when phosphorylated at Ser670.
RRID:
AB_2847011
Cite Format: Affinity Biosciences Cat# AF3697, RRID:AB_2847011.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ADRBK1; Adrenergic beta receptor kinase 1; ARBK1_HUMAN; BARK; BARK1; Beta adrenergic receptor kinase 1; Beta ARK 1; Beta ARK1; Beta-adrenergic receptor kinase 1; Beta-ARK-1; FLJ16718; G protein coupled receptor kinase 2; G-protein coupled receptor kinase 2; GRK2;

Immunogens

Immunogen:

A synthesized peptide derived from human GRK2 around the phosphorylation site of Ser670.

Uniprot:

P25098(ARBK1_HUMAN) >>Visit HPA database.

Gene(ID):
GRK2(156)
Expression:
P25098 ARBK1_HUMAN:

Expressed in peripheral blood leukocytes.

Sequence:
MADLEAVLADVSYLMAMEKSKATPAARASKKILLPEPSIRSVMQKYLEDRGEVTFEKIFSQKLGYLLFRDFCLNHLEEARPLVEFYEEIKKYEKLETEEERVARSREIFDSYIMKELLACSHPFSKSATEHVQGHLGKKQVPPDLFQPYIEEICQNLRGDVFQKFIESDKFTRFCQWKNVELNIHLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMSYAFHTPDKLSFILDLMNGGDLHYHLSQHGVFSEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGVAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTMAVELPDSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVKESPFFRSLDWQMVFLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDSDQELYRNFPLTISERWQQEVAETVFDTINAETDRLEARKKAKNKQLGHEEDYALGKDCIMHGYMSKMGNPFLTQWQRRYFYLFPNRLEWRGEGEAPQSLLTMEEIQSVEETQIKERKCLLLKIRGGKQFILQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPRSPVVELSKVPLVQRGSANGL

Research Backgrounds

Function:

Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner. Positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by facilitating the trafficking of SMO into the cilium and the stimulation of SMO activity (By similarity).

Subcellular Location:

Cytoplasm. Cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in peripheral blood leukocytes.

Family&Domains:

The PH domain binds anionic phospholipids and helps recruiting ADRBK1 from the cytoplasm to plasma membrane close to activated receptors. It mediates binding to G protein beta and gamma subunits, competing with G-alpha subunits and other G-betagamma effectors.

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway.   (View pathway)

· Human Diseases > Substance dependence > Morphine addiction.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Glutamatergic synapse.

· Organismal Systems > Sensory system > Olfactory transduction.

References

1). Low-dose ethanol consumption inhibits neutrophil extracellular traps formation to alleviate rheumatoid arthritis. Communications biology, 2023 (PubMed: 37884797) [IF=5.9]

Application: WB    Species: Human    Sample: dHL-60 cells

Fig. 6 Acetate reduces ERS in neutrophils and inhibits the formation of NETs. a Scanning electron microscopy observation of treated neutrophils (Scale Bar: 2 μm). i: Neutrophil-like differentiation of HL-60 (dHL-60) cells that developed NETs after 4 h of PMA treatment in vitro. ii: There were no NETs in dHL-60 cells treated with acetate and PMA. b Immunofluorescence staining of Sytox-green (green) and MPO (red) in dHL-60 cells, showing that NETs are reduced under sodium acetate treatment (Scale Bar: 50 μm and 25 μm). c Immunofluorescence staining of MPO (green) and p-IRE1 (red) in dHL-60 cells, showing that MPO and p-IRE1 co-localization is reduced under sodium acetate treatment (Scale Bar: 10 μm and 50 μm). d The effects of GRP78/p-IRE1/IRE1/CHOP/ATF6/XBP1/H3cit/MPO in dHL-60 cells exposed to different concentrations of acetate. Data are representative of three independent experiments. e The effects of H3cit/MPO in dHL-60 cells exposed to different concentrations of ethanol. Data are representative of three independent experiments. f The effects of acetate receptor GPR43 and its downstream proteins in dHL-60 cells after stimulated by TG. Data are representative of three independent experiments. g The effects of GRP78/p-IRE1/IRE1/CHOP/ATF6/XBP1/H3cit/MPO in dHL-60 cells after stimulated by TG. Data are representative of three independent experiments. h Immunoprecipitation analysis of ubiquitination of endogenous GRK2 in dHL-60 cells. Data are representative of three independent experiments.
2). The ROS/GRK2/HIF-1α/NLRP3 Pathway Mediates Pyroptosis of Fibroblast-Like Synoviocytes and the Regulation of Monomer Derivatives of Paeoniflorin. Oxidative Medicine and Cellular Longevity, 2022 (PubMed: 35132350)

Application: WB    Species: Human    Sample:

Figure 6 Monomeric derivative of paeoniflorin (MDP) inhibits the expression of hypoxia-inducible factor (HIF)-1α through targeted inhibition of GRK2 S670 phosphorylation. (a) Molecular docking modeling of the compound MDP and G protein-coupled receptor kinase 2 (GRK2), the small molecule, and the critical interaction of 3KRW (human GRK2 in complex with Gbetgamma subunits and balanol) are represented by sticks. (b) A schematic representation of the binding mode of MDP in the GRK2 binding site of 3KRW. (c, d) FLS were treated with or without MDP and exposed to hypoxia for 2 h. Immunoblot analysis and quantification of GRK2, p-GRK2 (S670), and HIF-1α. The values are shown as the mean ± standard error of the mean (n = 3). ∗p < 0.05 compared with the control group and #p < 0.05 compared with the hypoxia group.
3). Alcohol Consumption Alleviates Rheumatoid Arthritis by Promoting Acetate Production and Inhibiting NETs Formation. , 2023

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