Phospho-MARK3 (Thr507) Antibody - #AF3686

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Product: Phospho-MARK3 (Thr507) Antibody
Catalog: AF3686
Description: Rabbit polyclonal antibody to Phospho-MARK3 (Thr507)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 89kD,84kD,83kD(Calculated).
Uniprot: Q9P0L2 | P27448 | Q96L34
RRID: AB_2847000

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MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-MARK3 (Thr507) Antibody detects endogenous levels of MARK3 only when phosphorylated at Thr507.
RRID:
AB_2847000
Cite Format: Affinity Biosciences Cat# AF3686, RRID:AB_2847000.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C-TAK1; Cdc25C associated protein kinase 1; Cdc25C-associated protein kinase 1; cTAK1; ELKL motif kinase 2; EMK-2; Emk2; ETK 1; KIAA4230; KP78; MAP microtubule affinity regulating kinase 3; MAP/microtubule affinity-regulating kinase 3; Mark3; MARK3_HUMAN; Par 1a; PAR1A; Protein kinase STK10; Ser/Thr protein kinase PAR-1; Serine threonine protein kinase p78; Serine/threonine-protein kinase p78; SerThr protein kinase PAR 1; FLJ90097; KIAA1860; MAP / microtubule affinity regulating kinase 4; MAP / microtubule affinity regulating kinase 4L; MAP/microtubule affinity regulating kinase 4; MAP/microtubule affinity regulating kinase like 1; MAP/microtubule affinity-regulating kinase 4; MAP/microtubule affinity-regulating kinase-like 1; MARK 4; MARK 4L; MARK L1; MARK4; MARK4 serine / threonine protein kinase; MARK4 serine/threonine protein kinase; MARK4_HUMAN; MARK4S; MARKL 1; MARKL1; Nbla00650; Putative protein product of Nbla00650; KIAA1477; MAP/microtubule affinity regulating kinase 1; MAP/microtubule affinity-regul

Immunogens

Immunogen:

A synthesized peptide derived from human MARK3 around the phosphorylation site of Thr507.

Uniprot:

Q9P0L2(MARK1_HUMAN) >>Visit HPA database.

P27448(MARK3_HUMAN) >>Visit HPA database.

Q96L34(MARK4_HUMAN) >>Visit HPA database.

Gene(ID):
MARK1(4139) | MARK3(4140) | MARK4(57787)
Expression:
Q9P0L2 MARK1_HUMAN:

Highly expressed in heart, skeletal muscle, brain, fetal brain and fetal kidney.

P27448 MARK3_HUMAN:

Ubiquitous.

Q96L34 MARK4_HUMAN:

Ubiquitous. Isoform 2 is brain-specific (PubMed:11326310). Expressed at highest levels in brain and testis. Also expressed in heart, lung, liver, muscle, kidney and spleen (PubMed:14594945).

Sequence:
MSARTPLPTVNERDTENHTSVDGYTEPHIQPTKSSSRQNIPRCRNSITSATDEQPHIGNYRLQKTIGKGNFAKVKLARHVLTGREVAVKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKYIVHRDLKAENLLLDGDMNIKIADFGFSNEFTVGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKLLVLNPIKRGSLEQIMKDRWMNVGHEEEELKPYTEPDPDFNDTKRIDIMVTMGFARDEINDALINQKYDEVMATYILLGRKPPEFEGGESLSSGNLCQRSRPSSDLNNSTLQSPAHLKVQRSISANQKQRRFSDHAGPSIPPAVSYTKRPQANSVESEQKEEWDKDVARKLGSTTVGSKSEMTASPLVGPERKKSSTIPSNNVYSGGSMARRNTYVCERTTDRYVALQNGKDSSLTEMSVSSISSAGSSVASAVPSARPRHQKSMSTSGHPIKVTLPTIKDGSEAYRPGTTQRVPAASPSAHSISTATPDRTRFPRGSSSRSTFHGEQLRERRSVAYNGPPASPSHETGAFAHARRGTSTGIISKITSKFVRRDPSEGEASGRTDTSRSTSGEPKERDKEEGKDSKPRSLRFTWSMKTTSSMDPNDMMREIRKVLDANNCDYEQKERFLLFCVHGDARQDSLVQWEMEVCKLPRLSLNGVRFKRISGTSIAFKNIASKIANELKL

MSTRTPLPTVNERDTENHTSHGDGRQEVTSRTSRSGARCRNSIASCADEQPHIGNYRLLKTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLIMEYASGGEVFDYLVAHGRMKEKEARSKFRQIVSAVQYCHQKRIVHRDLKAENLLLDADMNIKIADFGFSNEFTVGGKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKRFLVLNPIKRGTLEQIMKDRWINAGHEEDELKPFVEPELDISDQKRIDIMVGMGYSQEEIQESLSKMKYDEITATYLLLGRKSSELDASDSSSSSNLSLAKVRPSSDLNNSTGQSPHHKVQRSVSSSQKQRRYSDHAGPAIPSVVAYPKRSQTSTADSDLKEDGISSRKSSGSAVGGKGIAPASPMLGNASNPNKADIPERKKSSTVPSSNTASGGMTRRNTYVCSERTTADRHSVIQNGKENSTIPDQRTPVASTHSISSAATPDRIRFPRGTASRSTFHGQPRERRTATYNGPPASPSLSHEATPLSQTRSRGSTNLFSKLTSKLTRRNMSFRFIKRLPTEYERNGRYEGSSRNVSAEQKDENKEAKPRSLRFTWSMKTTSSMDPGDMMREIRKVLDANNCDYEQRERFLLFCVHGDGHAENLVQWEMEVCKLPRLSLNGVRFKRISGTSIAFKNIASKIANELKL

MSSRTVLAPGNDRNSDTHGTLGSGRSSDKGPSWSSRSLGARCRNSIASCPEEQPHVGNYRLLRTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPSSLQKLFREVRIMKGLNHPNIVKLFEVIETEKTLYLVMEYASAGEVFDYLVSHGRMKEKEARAKFRQIVSAVHYCHQKNIVHRDLKAENLLLDAEANIKIADFGFSNEFTLGSKLDTFCGSPPYAAPELFQGKKYDGPEVDIWSLGVILYTLVSGSLPFDGHNLKELRERVLRGKYRVPFYMSTDCESILRRFLVLNPAKRCTLEQIMKDKWINIGYEGEELKPYTEPEEDFGDTKRIEVMVGMGYTREEIKESLTSQKYNEVTATYLLLGRKTEEGGDRGAPGLALARVRAPSDTTNGTSSSKGTSHSKGQRSSSSTYHRQRRHSDFCGPSPAPLHPKRSPTSTGEAELKEERLPGRKASCSTAGSGSRGLPPSSPMVSSAHNPNKAEIPERRKDSTSTPNNLPPSMMTRRNTYVCTERPGAERPSLLPNGKENSSGTPRVPPASPSSHSLAPPSGERSRLARGSTIRSTFHGGQVRDRRAGGGGGGGVQNGPPASPTLAHEAAPLPAGRPRPTTNLFTKLTSKLTRRVADEPERIGGPEVTSCHLPWDQTETAPRLLRFPWSVKLTSSRPPEALMAALRQATAAARCRCRQPQPFLLACLHGGAGGPEPLSHFEVEVCQLPRPGLRGVLFRRVAGTALAFRTLVTRISNDLEL

PTMs - Q9P0L2/P27448/Q96L34 As Substrate

Site PTM Type Enzyme
S15 Phosphorylation
T17 Phosphorylation
T20 Phosphorylation
S23 Phosphorylation
S26 Phosphorylation
S27 Phosphorylation
K29 Ubiquitination
S45 Phosphorylation
S48 Phosphorylation
R60 Methylation
T64 Phosphorylation
K67 Ubiquitination
K88 Ubiquitination
K92 Ubiquitination
K102 Ubiquitination
K111 Ubiquitination
K183 Ubiquitination
T214 Phosphorylation Q15831 (STK11)
S218 Phosphorylation
K230 Ubiquitination
K297 Ubiquitination
K306 Ubiquitination
K320 Ubiquitination
K356 Ubiquitination
S391 Phosphorylation
T393 Phosphorylation
T394 Phosphorylation
S404 Phosphorylation
S423 Phosphorylation
S438 Phosphorylation
T440 Phosphorylation
S441 Phosphorylation
T442 Phosphorylation
S458 Phosphorylation
S496 Phosphorylation
S504 Phosphorylation
T507 Phosphorylation
T511 Phosphorylation
S543 Phosphorylation
S545 Phosphorylation
S546 Phosphorylation
R561 Methylation
S563 Phosphorylation
T564 Phosphorylation
S567 Phosphorylation
T568 Phosphorylation
S594 Phosphorylation
K622 Ubiquitination
Site PTM Type Enzyme
T3 Phosphorylation
T5 Phosphorylation
S42 Phosphorylation
S45 Phosphorylation
T61 Phosphorylation
K64 Ubiquitination
K85 Ubiquitination
K89 Ubiquitination
T90 Phosphorylation P11309 (PIM1)
T95 Phosphorylation P11309 (PIM1)
S96 Phosphorylation P11309 (PIM1)
K180 Ubiquitination
T211 Phosphorylation Q15831 (STK11)
S215 Phosphorylation Q15831 (STK11)
K227 Ubiquitination
S238 Phosphorylation
K294 Ubiquitination
K303 Ubiquitination
K317 Ubiquitination
K330 Ubiquitination
Y340 Phosphorylation
S341 Phosphorylation
K367 Ubiquitination
S374 Phosphorylation
S376 Phosphorylation
S377 Phosphorylation
S378 Phosphorylation
S379 Phosphorylation
S380 Phosphorylation
S383 Phosphorylation
S396 Phosphorylation
T397 Phosphorylation
S400 Phosphorylation
Y418 Phosphorylation
S419 Phosphorylation
Y432 Phosphorylation
T438 Phosphorylation
S455 Phosphorylation
S456 Phosphorylation
S458 Phosphorylation
S469 Phosphorylation
S476 Phosphorylation
S489 Phosphorylation
S490 Phosphorylation
T491 Phosphorylation
S494 Phosphorylation
S499 Phosphorylation
T503 Phosphorylation
T507 Phosphorylation P17612 (PRKACA)
Y508 Phosphorylation
S511 Phosphorylation
S520 Phosphorylation
T530 Phosphorylation
T536 Phosphorylation
S540 Phosphorylation
T541 Phosphorylation
S543 Phosphorylation
S545 Phosphorylation
S546 Phosphorylation
T549 Phosphorylation
S563 Phosphorylation
T564 Phosphorylation P41743 (PRKCI) , Q05513 (PRKCZ)
T574 Phosphorylation
T576 Phosphorylation
Y577 Phosphorylation
S583 Phosphorylation
S585 Phosphorylation
S587 Phosphorylation
T591 Phosphorylation
S594 Phosphorylation
T596 Phosphorylation
S598 Phosphorylation
R599 Methylation
S601 Phosphorylation
S606 Phosphorylation
S610 Phosphorylation
K611 Ubiquitination
S618 Phosphorylation
S643 Phosphorylation
S657 Phosphorylation
K681 Ubiquitination
Y690 Phosphorylation
S724 Phosphorylation
K741 Ubiquitination
S745 Phosphorylation
Site PTM Type Enzyme
T5 Phosphorylation
S36 Phosphorylation
S46 Phosphorylation
T65 Phosphorylation
K68 Ubiquitination
K89 Ubiquitination
K93 Ubiquitination
T208 Phosphorylation
T215 Phosphorylation Q15831 (STK11)
S219 Phosphorylation
K231 Ubiquitination
K307 Ubiquitination
S382 Phosphorylation
S393 Phosphorylation
S399 Phosphorylation
S403 Phosphorylation
S414 Phosphorylation
S423 Phosphorylation
S444 Phosphorylation
S463 Phosphorylation
S468 Phosphorylation
S470 Phosphorylation
S475 Phosphorylation
Y494 Phosphorylation
S498 Phosphorylation
T504 Phosphorylation
Y505 Phosphorylation
S524 Phosphorylation
S554 Phosphorylation
S556 Phosphorylation
S573 Phosphorylation
T580 Phosphorylation
T581 Phosphorylation
S588 Phosphorylation
S590 Phosphorylation
S593 Phosphorylation
S595 Phosphorylation
T596 Phosphorylation
T598 Phosphorylation
S612 Phosphorylation
T613 Phosphorylation
S624 Phosphorylation
S633 Phosphorylation
R646 Methylation
T648 Phosphorylation
S649 Phosphorylation
S679 Phosphorylation
S681 Phosphorylation
S699 Phosphorylation
T708 Phosphorylation
T709 Phosphorylation
S710 Phosphorylation
S711 Phosphorylation
K723 Ubiquitination
S766 Phosphorylation
K783 Ubiquitination
S787 Phosphorylation

PTMs - Q9P0L2/P27448/Q96L34 As Enzyme

Substrate Site Source
Q96L34-2 (MARK4) S26 Uniprot
Q96L34-2 (MARK4) T214 Uniprot
Substrate Site Source
P27448-3 (MARK3) S598 Uniprot
P27448-3 (MARK3) S601 Uniprot
P27448-3 (MARK3) T602 Uniprot
P27448-3 (MARK3) S606 Uniprot
P30307 (CDC25C) S216 Uniprot
P41236 (PPP1R2) S72 Uniprot
Q8WUI4 (HDAC7) S155 Uniprot
Substrate Site Source
P10636-2 (MAPT) S204 Uniprot
P10636-8 (MAPT) T212 Uniprot
P10636-8 (MAPT) S214 Uniprot
P10636-8 (MAPT) T217 Uniprot
P10636-8 (MAPT) T231 Uniprot
P10636-2 (MAPT) S235 Uniprot
P10636-6 (MAPT) S247 Uniprot
P10636-8 (MAPT) S262 Uniprot
P10636-6 (MAPT) S266 Uniprot
P10636-2 (MAPT) S267 Uniprot
P10636-8 (MAPT) S293 Uniprot
P10636-6 (MAPT) S298 Uniprot
P10636-8 (MAPT) S305 Uniprot
P10636-8 (MAPT) S320 Uniprot
P10636-8 (MAPT) S324 Uniprot
P10636-8 (MAPT) S356 Uniprot
P10636-8 (MAPT) S396 Uniprot
P10636-8 (MAPT) S422 Uniprot
P10636 (MAPT) S579 Uniprot
P10636 (MAPT) S641 Uniprot
P10636 (MAPT) S673 Uniprot
P11137-2 (MAP2) S184 Uniprot
P11137-2 (MAP2) S323 Uniprot
P11137-2 (MAP2) S354 Uniprot
P11137 (MAP2) S385 Uniprot
P11137-2 (MAP2) S439 Uniprot
P11137-2 (MAP2) S443 Uniprot
P11137-2 (MAP2) S446 Uniprot
P11137-1 (MAP2) S1540 Uniprot
P11137-1 (MAP2) S1679 Uniprot
P11137-1 (MAP2) S1710 Uniprot
P11137-1 (MAP2) S1795 Uniprot
P11137-1 (MAP2) S1799 Uniprot
P11137-1 (MAP2) S1802 Uniprot
P27816-1 (MAP4) S928 Uniprot
P27816 (MAP4) S941 Uniprot
P27816-1 (MAP4) S1073 Uniprot

Research Backgrounds

Function:

Serine/threonine-protein kinase. Involved in cell polarity and microtubule dynamics regulation. Phosphorylates DCX, MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU. Involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3).

PTMs:

Phosphorylation at Thr-613 by PRKCZ/aPKC in polarized epithelial cells inhibits the kinase activity (By similarity). Phosphorylated at Thr-215 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-215 by TAOK1 activates the kinase activity, leading to phosphorylation and detachment of MAPT/TAU from microtubules. Phosphorylation at Ser-219 by GSK3-beta (GSK3B) inhibits the kinase activity.

Subcellular Location:

Cell membrane>Peripheral membrane protein. Cytoplasm>Cytoskeleton. Cytoplasm. Cell projection>Dendrite.
Note: Appears to localize to an intracellular network.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in heart, skeletal muscle, brain, fetal brain and fetal kidney.

Subunit Structure:

Interacts with MAPT/TAU.

Family&Domains:

The UBA domain does not seem to bind ubiquitin and ubiquitin-like and might play a role in regulating the enzyme conformation and localization. Activation of the kinase activity following phosphorylation at Thr-208 is accompanied by a conformational change that alters the orientation of the UBA domain with respect to the catalytic domain (By similarity).

The KA1 domain mediates binding to phospholipids and targeting to membranes. Binds phosphatidic acid (PA), phosphatidylserine (PtdSer) and phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2).

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.

Function:

Serine/threonine-protein kinase. Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU. Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1.

PTMs:

Phosphorylated at Thr-211 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-564 by PRKCZ/aPKC inhibits the kinase activity.

Subcellular Location:

Cell membrane>Peripheral membrane protein. Cell projection>Dendrite. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with MAPT/TAU. Interacts with DLG5 (via coiled-coil domain). Interacts with STK3/MST2 and STK4/MST1 in the presence of DLG5. Interacts with YWHAB, YWHAG, YWHAQ and YWHAZ.

Family&Domains:

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.

Function:

Serine/threonine-protein kinase. Phosphorylates the microtubule-associated protein MAPT/TAU. Also phosphorylates the microtubule-associated proteins MAP2 and MAP4. Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles. Required for the initiation of axoneme extension during cilium assembly. Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro. Plays a role in cell cycle progression, specifically in the G1/S checkpoint. Reduces neuronal cell survival. Plays a role in energy homeostasis by regulating satiety and metabolic rate (By similarity). Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway (By similarity). Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation.

PTMs:

Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X.

Phosphorylated at Thr-214 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylated throughout the cell cycle.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Microtubule organizing center. Cytoplasm>Cytoskeleton>Cilium basal body. Cytoplasm>Cytoskeleton>Cilium axoneme. Cytoplasm. Cell projection>Dendrite.
Note: Localized at the tips of neurite-like processes in differentiated neuroblast cells. Detected in the cytoplasm and neuropil of the hippocampus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Isoform 2 is brain-specific. Expressed at highest levels in brain and testis. Also expressed in heart, lung, liver, muscle, kidney and spleen.

Subunit Structure:

Interacts with MAPT/TAU. Interacts with gamma-tubulin. Interacts with ODF2. Interacts with USP9X. Interacts with YWHAQ.

Family&Domains:

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.

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