Phospho-CD171/N-CAML1 (Ser1181) Antibody

Product:	Phospho-CD171/N-CAML1 (Ser1181) Antibody
Catalog:	AF3683
Description:	Rabbit polyclonal antibody to Phospho-CD171/N-CAML1 (Ser1181)
Application:	ELISA(peptide)
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	140kD(Calculated).
Uniprot:	P32004
RRID:	AB_2846997

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Phospho-CD171/N-CAML1 (Ser1181) Antibody detects endogenous levels of CD171/N-CAML1 only when phosphorylated at Ser1181.

RRID:

AB_2846997
Cite Format: Affinity Biosciences Cat# AF3683, RRID:AB_2846997.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Antigen identified by monoclonal antibody R1; CAML1; CD171; CD171 antigen; HSAS; HSAS1; Hyd; L1; L1 cell adhesion molecule; L1-NCAM; L1cam; L1CAM_HUMAN; MASA; MIC5; N CAML1; N-CAM-L1; NCAM-L1; NCAML1; Nerve-growth factor-inducible large external glycoprotein; Neural cell adhesion molecule L1; NILE; OTTHUMP00000025992; S10; SPG1;

Immunogens

Immunogen:

A synthesized peptide derived from human CD171/N-CAML1 around the phosphorylation site of Ser1181.

Uniprot:

P32004(L1CAM_HUMAN) >>Visit HPA database.

Gene(ID):

L1CAM(3897)

Sequence:

P32004 L1CAM_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVVALRYVWPLLLCSPCLLIQIPEEYEGHHVMEPPVITEQSPRRLVVFPTDDISLKCEASGKPEVQFRWTRDGVHFKPKEELGVTVYQSPHSGSFTITGNNSNFAQRFQGIYRCFASNKLGTAMSHEIRLMAEGAPKWPKETVKPVEVEEGESVVLPCNPPPSAEPLRIYWMNSKILHIKQDERVTMGQNGNLYFANVLTSDNHSDYICHAHFPGTRTIIQKEPIDLRVKATNSMIDRKPRLLFPTNSSSHLVALQGQPLVLECIAEGFPTPTIKWLRPSGPMPADRVTYQNHNKTLQLLKVGEEDDGEYRCLAENSLGSARHAYYVTVEAAPYWLHKPQSHLYGPGETARLDCQVQGRPQPEVTWRINGIPVEELAKDQKYRIQRGALILSNVQPSDTMVTQCEARNRHGLLLANAYIYVVQLPAKILTADNQTYMAVQGSTAYLLCKAFGAPVPSVQWLDEDGTTVLQDERFFPYANGTLGIRDLQANDTGRYFCLAANDQNNVTIMANLKVKDATQITQGPRSTIEKKGSRVTFTCQASFDPSLQPSITWRGDGRDLQELGDSDKYFIEDGRLVIHSLDYSDQGNYSCVASTELDVVESRAQLLVVGSPGPVPRLVLSDLHLLTQSQVRVSWSPAEDHNAPIEKYDIEFEDKEMAPEKWYSLGKVPGNQTSTTLKLSPYVHYTFRVTAINKYGPGEPSPVSETVVTPEAAPEKNPVDVKGEGNETTNMVITWKPLRWMDWNAPQVQYRVQWRPQGTRGPWQEQIVSDPFLVVSNTSTFVPYEIKVQAVNSQGKGPEPQVTIGYSGEDYPQAIPELEGIEILNSSAVLVKWRPVDLAQVKGHLRGYNVTYWREGSQRKHSKRHIHKDHVVVPANTTSVILSGLRPYSSYHLEVQAFNGRGSGPASEFTFSTPEGVPGHPEALHLECQSNTSLLLRWQPPLSHNGVLTGYVLSYHPLDEGGKGQLSFNLRDPELRTHNLTDLSPHLRYRFQLQATTKEGPGEAIVREGGTMALSGISDFGNISATAGENYSVVSWVPKEGQCNFRFHILFKALGEEKGGASLSPQYVSYNQSSYTQWDLQPDTDYEIHLFKERMFRHQMAVKTNGTGRVRLPPAGFATEGWFIGFVSAIILLLLVLLILCFIKRSKGGKYSVKDKEDTQVDSEARPMKDETFGEYRSLESDNEEKAFGSSQPSLNGDIKPLGSDDSLADYGGSVDVQFNEDGSFIGQYSGKKEKEAAGGNDSSGATSPINPAVALE

PTMs - P32004 As Substrate

P32004

Site	PTM Type	Enzyme	Source
	Phosphorylation		Uniprot
K56	Ubiquitination		Uniprot
K77	Ubiquitination		Uniprot
K140	Ubiquitination		Uniprot
K144	Ubiquitination		Uniprot
S153	Phosphorylation		Uniprot
S163	Phosphorylation		Uniprot
K180	Ubiquitination		Uniprot
K222	Ubiquitination		Uniprot
K230	Ubiquitination		Uniprot
K295	Ubiquitination		Uniprot
K301	Ubiquitination		Uniprot
K338	Ubiquitination		Uniprot
K378	Ubiquitination		Uniprot
Y418	Phosphorylation		Uniprot
Y420	Phosphorylation		Uniprot
K515	Ubiquitination		Uniprot
T518	Phosphorylation		Uniprot
T521	Phosphorylation		Uniprot
T527	Phosphorylation		Uniprot
K530	Acetylation		Uniprot
K568	Ubiquitination		Uniprot
S611	Phosphorylation		Uniprot
K655	Ubiquitination		Uniprot
K661	Ubiquitination		Uniprot
K667	Ubiquitination		Uniprot
N671	N-Glycosylation		Uniprot
K694	Ubiquitination		Uniprot
K716	Ubiquitination		Uniprot
Y784	Phosphorylation		Uniprot
K998	Ubiquitination		Uniprot
K1150	Ubiquitination		Uniprot
Y1151	Phosphorylation		Uniprot
S1152	Phosphorylation		Uniprot
K1154	Ubiquitination		Uniprot
K1156	Ubiquitination		Uniprot
S1163	Phosphorylation		Uniprot
K1169	Ubiquitination		Uniprot
T1172	Phosphorylation	P68400 (CSNK2A1) , P17252 (PRKCA)	Uniprot
Y1176	Phosphorylation	P12931 (SRC)	Uniprot
S1181	Phosphorylation	P68400 (CSNK2A1)	Uniprot
S1190	Phosphorylation		Uniprot
S1191	Phosphorylation		Uniprot
S1194	Phosphorylation		Uniprot
S1204	Phosphorylation	P28482 (MAPK1)	Uniprot
Y1211	Phosphorylation		Uniprot
Y1229	Phosphorylation	P54753 (EPHB3)	Uniprot
K1235	Ubiquitination		Uniprot
S1243	Phosphorylation		Uniprot
S1244	Phosphorylation		Uniprot
T1247	Phosphorylation		Uniprot
S1248	Phosphorylation	P28482 (MAPK1)	Uniprot

Research Backgrounds

P32004_L1CAM_HUMAN

Function:

Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Cell projection>Growth cone. Cell projection>Axon. Cell projection>Dendrite.
Note: Colocalized with SHTN1 in close apposition with actin filaments in filopodia and lamellipodia of axonalne growth cones of hippocampal neurons (By similarity). In neurons, detected predominantly in axons and cell body, weak localization to dendrites (PubMed:20621658).

Subunit Structure:

Interacts with SHTN1; the interaction occurs in axonal growth cones.

Family&Domains:

Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)

· Organismal Systems > Development > Axon guidance. (View pathway)

Restrictive clause

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For Research Use Only.
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Phospho-CD171/N-CAML1 (Ser1181) Antibody - #AF3683