Phospho-CD50/ICAM-3 (Ser518) Antibody

Product:	Phospho-CD50/ICAM-3 (Ser518) Antibody
Catalog:	AF3682
Description:	Rabbit polyclonal antibody to Phospho-CD50/ICAM-3 (Ser518)
Application:	ELISA(peptide)
Reactivity:	Human
Mol.Wt.:	60kD(Calculated).
Uniprot:	P32942
RRID:	AB_2846996

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

Phospho-CD50/ICAM-3 (Ser518) Antibody detects endogenous levels of CD50/ICAM-3 only when phosphorylated at Ser518.

RRID:

AB_2846996
Cite Format: Affinity Biosciences Cat# AF3682, RRID:AB_2846996.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD50; CDW 50; CDw50; ICAM 3; ICAM-3; ICAM-R; ICAM3; ICAM3_HUMAN; ICAMR; Intercellular adhesion molecule 3;

Immunogens

Immunogen:

A synthesized peptide derived from human CD50/ICAM-3 around the phosphorylation site of Ser518.

Uniprot:

P32942(ICAM3_HUMAN) >>Visit HPA database.

Gene(ID):

ICAM3(3385)

Expression:

P32942 ICAM3_HUMAN:

Leukocytes.

Sequence:

P32942 ICAM3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MATMVPSVLWPRACWTLLVCCLLTPGVQGQEFLLRVEPQNPVLSAGGSLFVNCSTDCPSSEKIALETSLSKELVASGMGWAAFNLSNVTGNSRILCSVYCNGSQITGSSNITVYRLPERVELAPLPPWQPVGQNFTLRCQVEDGSPRTSLTVVLLRWEEELSRQPAVEEPAEVTATVLASRDDHGAPFSCRTELDMQPQGLGLFVNTSAPRQLRTFVLPVTPPRLVAPRFLEVETSWPVDCTLDGLFPASEAQVYLALGDQMLNATVMNHGDTLTATATATARADQEGAREIVCNVTLGGERREARENLTVFSFLGPIVNLSEPTAHEGSTVTVSCMAGARVQVTLDGVPAAAPGQPAQLQLNATESDDGRSFFCSATLEVDGEFLHRNSSVQLRVLYGPKIDRATCPQHLKWKDKTRHVLQCQARGNPYPELRCLKEGSSREVPVGIPFFVNVTHNGTYQCQASSSRGKYTLVVVMDIEAGSSHFVPVFVAVLLTLGVVTIVLALMYVFREHQRSGSYHVREESTYLPLTSMQPTEAMGEEPSRAE

PTMs - P32942 As Substrate

P32942

Site	PTM Type	Enzyme	Source
N52	N-Glycosylation		Uniprot
N84	N-Glycosylation		Uniprot
N87	N-Glycosylation		Uniprot
N110	N-Glycosylation		Uniprot
N134	N-Glycosylation		Uniprot
T151	Phosphorylation		Uniprot
N206	N-Glycosylation		Uniprot
N295	N-Glycosylation		Uniprot
N363	N-Glycosylation		Uniprot
K401	Ubiquitination		Uniprot
K412	Ubiquitination		Uniprot
S516	Phosphorylation		Uniprot
S518	Phosphorylation	Q04759 (PRKCQ)	Uniprot
Y519	Phosphorylation		Uniprot
S525	Phosphorylation		Uniprot
T526	Phosphorylation		Uniprot
Y527	Phosphorylation		Uniprot
T531	Phosphorylation		Uniprot
S532	Phosphorylation		Uniprot
T536	Phosphorylation		Uniprot

Research Backgrounds

P32942_ICAM3_HUMAN

Function:

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages.

PTMs:

Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues.

N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Tissue Specificity:

Leukocytes.

Subunit Structure:

Interacts with moesin/MSN.

Family&Domains:

Belongs to the immunoglobulin superfamily. ICAM family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)

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For Research Use Only.
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Phospho-CD50/ICAM-3 (Ser518) Antibody - #AF3682