Product: Phospho-HNF4 alpha (Ser304) Antibody
Catalog: AF3671
Description: Rabbit polyclonal antibody to Phospho-HNF4 alpha (Ser304)
Application: IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 53kD(Calculated).
Uniprot: P41235
RRID: AB_2846985

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 100ul $350 In stock
 200ul $450 In stock

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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-HNF4 alpha (Ser304) Antibody detects endogenous levels of HNF4 alpha only when phosphorylated at Ser304.
RRID:
AB_2846985
Cite Format: Affinity Biosciences Cat# AF3671, RRID:AB_2846985.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ39654; FRTS4; Hepatic nuclear factor 4 alpha; Hepatocyte nuclear factor 4 alpha; Hepatocyte nuclear factor 4; Hepatocyte nuclear factor 4-alpha; HNF 4 alpha; HNF 4; HNF-4-alpha; HNF4; HNF4A; HNF4A_HUMAN; HNF4a7; HNF4a8; HNF4a9; Hnf4alpha; HNF4alpha10/11/12; MODY 1; MODY; MODY1; NR2A1; NR2A21; Nuclear receptor subfamily 2 group A member 1; OTTHUMP00000031060; OTTHUMP00000031062; TCF 14; TCF; TCF-14; TCF14; Tcf4; Transcription factor 14, hepatic nuclear factor; Transcription factor 14; Transcription factor HNF 4; Transcription factor HNF-4; Transcription factor HNF4;

Immunogens

Immunogen:

A synthesized peptide derived from human HNF4 alpha around the phosphorylation site of Ser304.

Uniprot:
Gene(ID):
Sequence:
MRLSKTLVDMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGTNLNAPNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSRQITSPVSGINGDIRAKKIASIADVCESMKEQLLVLVEWAKYIPAFCELPLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYAYLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEWPRPRGQAATPETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQPTITKQEVI

PTMs - P41235 As Substrate

Site PTM Type Enzyme
Y23 Phosphorylation P12931 (SRC)
Y72 Phosphorylation
S75 Phosphorylation
S76 Phosphorylation
S95 Phosphorylation
S99 Phosphorylation
K106 Acetylation
K108 Acetylation
K126 Acetylation
K127 Acetylation
S138 Phosphorylation
T139 Phosphorylation
S142 Phosphorylation P17612 (PRKACA)
S143 Phosphorylation P17612 (PRKACA)
Y144 Phosphorylation
S147 Phosphorylation
S148 Phosphorylation
S151 Phosphorylation
T166 Phosphorylation
S167 Phosphorylation Q16539 (MAPK14) , Q15759 (MAPK11)
S170 Phosphorylation
S190 Phosphorylation
K234 Ubiquitination
S262 Phosphorylation
S265 Phosphorylation
Y286 Phosphorylation P12931 (SRC)
Y288 Phosphorylation P12931 (SRC)
S303 Phosphorylation Q13131 (PRKAA1)
K307 Ubiquitination
K309 Ubiquitination
S313 Phosphorylation Q13131 (PRKAA1)
S318 Phosphorylation
S378 Phosphorylation
T429 Phosphorylation
T432 Phosphorylation
S436 Phosphorylation
S441 Phosphorylation
S443 Phosphorylation
T455 Phosphorylation
K458 Acetylation
S461 Phosphorylation
T467 Phosphorylation
T469 Phosphorylation

Research Backgrounds

Function:

Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes. Activates the transcription of CYP2C38 (By similarity). Represses the CLOCK-ARNTL/BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells.

PTMs:

Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA.

Acetylation at Lys-458 lowers transcriptional activation by about two-fold.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimerization is required for HNF4-alpha to bind to its recognition site. Interacts with CLOCK, ARNTL, CRY1, CRY2, PER1 and PER2. Interacts with NR0B2/SHP; the resulting heterodimer is transcriptionnally inactive. Interacts with DDX3X; this interaction disrupts the interaction between HNF4 and NR0B2 that forms inactive heterodimers and enhances the formation of active HNF4 homodimers.

Family&Domains:

The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Belongs to the nuclear hormone receptor family. NR2 subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.

Restrictive clause

 

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For Research Use Only.
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