Phospho-GIRK1/KIR3.1/KCNJ3 (Ser185) Antibody

Product:	Phospho-GIRK1/KIR3.1/KCNJ3 (Ser185) Antibody
Catalog:	AF3661
Description:	Rabbit polyclonal antibody to Phospho-GIRK1/KIR3.1/KCNJ3 (Ser185)
Application:	ELISA(peptide)
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	57kD(Calculated).
Uniprot:	P48549
RRID:	AB_2846975

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Phospho-GIRK1/KIR3.1/KCNJ3 (Ser185) Antibody detects endogenous levels of GIRK1/KIR3.1/KCNJ3 only when phosphorylated at Ser185.

RRID:

AB_2846975
Cite Format: Affinity Biosciences Cat# AF3661, RRID:AB_2846975.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

G protein activated inward rectifier potassium channel 1; G protein-activated inward rectifier potassium channel 1; GIRK-1; GIRK1; Inward rectifier K(+) channel Kir3.1; Inwardly rectifying potassium channel KIR3.1; inwardly rectifying subfamily J member 3; IRK3; IRK3_HUMAN; Kcnf3; Kcnj3; KGA; KGB1; Kir3.1; Potassium channel; Potassium channel, inwardly rectifying subfamily J member 3; Potassium inwardly-rectifying channel, subfamily J, member 3;

Immunogens

Immunogen:

A synthesized peptide derived from human GIRK1/KIR3.1/KCNJ3 around the phosphorylation site of Ser185.

Uniprot:

P48549(KCNJ3_HUMAN) >>Visit HPA database.

Gene(ID):

KCNJ3(3760)

Sequence:

P48549 KCNJ3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSALRRKFGDDYQVVTTSSSGSGLQPQGPGQDPQQQLVPKKKRQRFVDKNGRCNVQHGNLGSETSRYLSDLFTTLVDLKWRWNLFIFILTYTVAWLFMASMWWVIAYTRGDLNKAHVGNYTPCVANVYNFPSAFLFFIETEATIGYGYRYITDKCPEGIILFLFQSILGSIVDAFLIGCMFIKMSQPKKRAETLMFSEHAVISMRDGKLTLMFRVGNLRNSHMVSAQIRCKLLKSRQTPEGEFLPLDQLELDVGFSTGADQLFLVSPLTICHVIDAKSPFYDLSQRSMQTEQFEIVVILEGIVETTGMTCQARTSYTEDEVLWGHRFFPVISLEEGFFKVDYSQFHATFEVPTPPYSVKEQEEMLLMSSPLIAPAITNSKERHNSVECLDGLDDITTKLPSKLQKITGREDFPKKLLRMSSTTSEKAYSLGDLPMKLQRISSVPGNSEEKLVSKTTKMLSDPMSQSVADLPPKLQKMAGGAARMEGNLPAKLRKMNSDRFT

PTMs - P48549 As Substrate

P48549

Site	PTM Type	Enzyme	Source
S62	Phosphorylation		Uniprot
S65	Phosphorylation		Uniprot
S69	Phosphorylation		Uniprot
T73	Phosphorylation		Uniprot
N119	N-Glycosylation		Uniprot
S369	Phosphorylation		Uniprot
S385	Phosphorylation	P17612 (PRKACA)	Uniprot
S401	Phosphorylation		Uniprot
T407	Phosphorylation		Uniprot
S420	Phosphorylation		Uniprot
K457	Acetylation		Uniprot

Research Backgrounds

P48549_KCNJ3_HUMAN

Function:

This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This receptor plays a crucial role in regulating the heartbeat.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Subunit Structure:

Associates with GIRK2, GIRK3 or GIRK4 to form a G-protein activated heteromultimer pore-forming unit. The resulting inward current is much larger (By similarity).

Family&Domains:

Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ3 subfamily.

Research Fields

· Human Diseases > Substance dependence > Morphine addiction.

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling. (View pathway)

· Organismal Systems > Nervous system > Glutamatergic synapse.

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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Phospho-GIRK1/KIR3.1/KCNJ3 (Ser185) Antibody - #AF3661