Product: Phospho-GIRK1/KIR3.1/KCNJ3 (Ser185) Antibody
Catalog: AF3661
Description: Rabbit polyclonal antibody to Phospho-GIRK1/KIR3.1/KCNJ3 (Ser185)
Application: ELISA(peptide)
Reactivity: Human, Mouse, Rat
Mol.Wt.: 57kD(Calculated).
Uniprot: P48549
RRID: AB_2846975

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 100ul $350 In stock
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Product Info

Source:
Rabbit
Application:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-GIRK1/KIR3.1/KCNJ3 (Ser185) Antibody detects endogenous levels of GIRK1/KIR3.1/KCNJ3 only when phosphorylated at Ser185.
RRID:
AB_2846975
Cite Format: Affinity Biosciences Cat# AF3661, RRID:AB_2846975.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

G protein activated inward rectifier potassium channel 1; G protein-activated inward rectifier potassium channel 1; GIRK-1; GIRK1; Inward rectifier K(+) channel Kir3.1; Inwardly rectifying potassium channel KIR3.1; inwardly rectifying subfamily J member 3; IRK3; IRK3_HUMAN; Kcnf3; Kcnj3; KGA; KGB1; Kir3.1; Potassium channel; Potassium channel, inwardly rectifying subfamily J member 3; Potassium inwardly-rectifying channel, subfamily J, member 3;

Immunogens

Immunogen:

A synthesized peptide derived from human GIRK1/KIR3.1/KCNJ3 around the phosphorylation site of Ser185.

Uniprot:
Gene(ID):
Sequence:
MSALRRKFGDDYQVVTTSSSGSGLQPQGPGQDPQQQLVPKKKRQRFVDKNGRCNVQHGNLGSETSRYLSDLFTTLVDLKWRWNLFIFILTYTVAWLFMASMWWVIAYTRGDLNKAHVGNYTPCVANVYNFPSAFLFFIETEATIGYGYRYITDKCPEGIILFLFQSILGSIVDAFLIGCMFIKMSQPKKRAETLMFSEHAVISMRDGKLTLMFRVGNLRNSHMVSAQIRCKLLKSRQTPEGEFLPLDQLELDVGFSTGADQLFLVSPLTICHVIDAKSPFYDLSQRSMQTEQFEIVVILEGIVETTGMTCQARTSYTEDEVLWGHRFFPVISLEEGFFKVDYSQFHATFEVPTPPYSVKEQEEMLLMSSPLIAPAITNSKERHNSVECLDGLDDITTKLPSKLQKITGREDFPKKLLRMSSTTSEKAYSLGDLPMKLQRISSVPGNSEEKLVSKTTKMLSDPMSQSVADLPPKLQKMAGGAARMEGNLPAKLRKMNSDRFT

PTMs - P48549 As Substrate

Site PTM Type Enzyme
S62 Phosphorylation
S65 Phosphorylation
S69 Phosphorylation
T73 Phosphorylation
N119 N-Glycosylation
S369 Phosphorylation
S385 Phosphorylation P17612 (PRKACA)
S401 Phosphorylation
T407 Phosphorylation
S420 Phosphorylation
K457 Acetylation

Research Backgrounds

Function:

This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This receptor plays a crucial role in regulating the heartbeat.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Associates with GIRK2, GIRK3 or GIRK4 to form a G-protein activated heteromultimer pore-forming unit. The resulting inward current is much larger (By similarity).

Family&Domains:

Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ3 subfamily.

Research Fields

· Human Diseases > Substance dependence > Morphine addiction.

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

· Organismal Systems > Nervous system > Glutamatergic synapse.

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

Restrictive clause

 

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For Research Use Only.
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