Phospho-FOXL2 (Ser263) Antibody - #AF3649

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Product: Phospho-FOXL2 (Ser263) Antibody
Catalog: AF3649
Description: Rabbit polyclonal antibody to Phospho-FOXL2 (Ser263)
Application: IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 39kD(Calculated).
Uniprot: P58012
RRID: AB_2846963

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Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-FOXL2 (Ser263) Antibody detects endogenous levels of FOXL2 only when phosphorylated at Ser263.
RRID:
AB_2846963
Cite Format: Affinity Biosciences Cat# AF3649, RRID:AB_2846963.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Blepharophimosis; Blepharophimosis epicanthus inversus and ptosis 1; Blepharophimosis epicanthus inversus and ptosis; BPES 1; BPES; BPES1; Epicanthus inversus and ptosis 1; Forkhead box L2; Forkhead box protein L2; Forkhead transcription factor FOXL2; FOX L2; FOXL 2; FOXL2; FOXL2_HUMAN; PFRK; PINTO; PITUITARY FORKHEAD FACTOR; POF 3; POF3;

Immunogens

Immunogen:

A synthesized peptide derived from human FOXL2 around the phosphorylation site of Ser263.

Uniprot:

P58012(FOXL2_HUMAN) >>Visit HPA database.

Gene(ID):
FOXL2(668)
Expression:
P58012 FOXL2_HUMAN:

In addition to its expression in the developing eyelid, it is transcribed very early in somatic cells of the developing gonad (before sex determination) and its expression persists in the follicular cells of the adult ovary.

Sequence:
MMASYPEPEDAAGALLAPETGRTVKEPEGPPPSPGKGGGGGGGTAPEKPDPAQKPPYSYVALIAMAIRESAEKRLTLSGIYQYIIAKFPFYEKNKKGWQNSIRHNLSLNECFIKVPREGGGERKGNYWTLDPACEDMFEKGNYRRRRRMKRPFRPPPAHFQPGKGLFGAGGAAGGCGVAGAGADGYGYLAPPKYLQSGFLNNSWPLPQPPSPMPYASCQMAAAAAAAAAAAAAAGPGSPGAAAVVKGLAGPAASYGPYTRVQSMALPPGVVNSYNGLGGPPAAPPPPPHPHPHPHAHHLHAAAAPPPAPPHHGAAAPPPGQLSPASPATAAPPAPAPTSAPGLQFACARQPELAMMHCSYWDHDSKTGALHSRLDL

PTMs - P58012 As Substrate

Site PTM Type Enzyme
S4 Phosphorylation
Y5 Phosphorylation
K25 Sumoylation
S33 Phosphorylation P49841 (GSK3B)
Y81 Phosphorylation
Y83 Phosphorylation
Y91 Phosphorylation
K96 Acetylation
K96 Ubiquitination
S101 Phosphorylation
S107 Phosphorylation
K114 Sumoylation
Y127 Phosphorylation
K140 Acetylation
Y186 Phosphorylation
S211 Phosphorylation
Y215 Phosphorylation
S238 Phosphorylation
Y258 Phosphorylation
S263 Phosphorylation Q13153 (PAK1)
S323 Phosphorylation
S326 Phosphorylation
T329 Phosphorylation
K366 Acetylation

Research Backgrounds

Function:

Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis through transcriptional repression of the Sertoli cell-promoting gene SOX9 (By similarity). Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen (By similarity). Is a regulator of CYP19 expression (By similarity). Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element (By similarity). Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2.

PTMs:

Sumoylated with SUMO1; sumoylation is required for transcriptional repression activity.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

In addition to its expression in the developing eyelid, it is transcribed very early in somatic cells of the developing gonad (before sex determination) and its expression persists in the follicular cells of the adult ovary.

Subunit Structure:

Interacts with ESR1 (By similarity). Interacts with SMAD3 (By similarity). Interacts with DDX20. Interacts with UBE2I/UBC9.

Restrictive clause

 

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For Research Use Only.
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