Phospho-MLK1/2 (Thr312/Thr266) Antibody - #AF3630

Product: Phospho-MLK1/2 (Thr312/Thr266) Antibody
Catalog: AF3630
Description: Rabbit polyclonal antibody to Phospho-MLK1/2 (Thr312/Thr266)
Application: ELISA(peptide)
Reactivity: Human, Mouse, Rat
Mol.Wt.: 122kD,104kD(Calculated).
Uniprot: P80192 | Q02779
RRID: AB_2846944

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-MLK1/2 (Thr312/Thr266) Antibody detects endogenous levels of MLK1/2 only when phosphorylated at Thr312/266.
RRID:
AB_2846944
Cite Format: Affinity Biosciences Cat# AF3630, RRID:AB_2846944.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

M3K9_HUMAN; Map3k9; MEKK9; Mitogen activated protein kinase kinase kinase 9; Mitogen-activated protein kinase kinase kinase 9; Mixed lineage kinase 1 (tyr and ser/thr specificity); Mixed lineage kinase 1; MLK1; PRKE1; M3K10_HUMAN; MAP3K 10; Map3k10; MEKK10; Mitogen activated protein kinase kinase kinase 10; Mitogen-activated protein kinase kinase kinase 10; Mixed lineage kinase 2; MKN28 derived nonreceptor type serine/threonine kinase; MKN28 kinase; MLK 2; MST; Protein kinase MST;

Immunogens

Immunogen:

A synthesized peptide derived from human MLK1/2 around the phosphorylation site of Thr312/266.

Uniprot:

P80192(M3K9_HUMAN) >>Visit HPA database.

Q02779(M3K10_HUMAN) >>Visit HPA database.

Gene(ID):
MAP3K9(4293) | MAP3K10(4294)
Expression:
P80192 M3K9_HUMAN:

Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin.

Q02779 M3K10_HUMAN:

Expressed in brain and skeletal muscle.

Sequence:
MEPSRALLGCLASAAAAAPPGEDGAGAGAEEEEEEEEEAAAAVGPGELGCDAPLPYWTAVFEYEAAGEDELTLRLGDVVEVLSKDSQVSGDEGWWTGQLNQRVGIFPSNYVTPRSAFSSRCQPGGEDPSCYPPIQLLEIDFAELTLEEIIGIGGFGKVYRAFWIGDEVAVKAARHDPDEDISQTIENVRQEAKLFAMLKHPNIIALRGVCLKEPNLCLVMEFARGGPLNRVLSGKRIPPDILVNWAVQIARGMNYLHDEAIVPIIHRDLKSSNILILQKVENGDLSNKILKITDFGLAREWHRTTKMSAAGTYAWMAPEVIRASMFSKGSDVWSYGVLLWELLTGEVPFRGIDGLAVAYGVAMNKLALPIPSTCPEPFAKLMEDCWNPDPHSRPSFTNILDQLTTIEESGFFEMPKDSFHCLQDNWKHEIQEMFDQLRAKEKELRTWEEELTRAALQQKNQEELLRRREQELAEREIDILERELNIIIHQLCQEKPRVKKRKGKFRKSRLKLKDGNRISLPSDFQHKFTVQASPTMDKRKSLINSRSSPPASPTIIPRLRAIQLTPGESSKTWGRSSVVPKEEGEEEEKRAPKKKGRTWGPGTLGQKELASGDEGSPQRREKANGLSTPSESPHFHLGLKSLVDGYKQWSSSAPNLVKGPRSSPALPGFTSLMEMEDEDSEGPGSGESRLQHSPSQSYLCIPFPRGEDGDGPSSDGIHEEPTPVNSATSTPQLTPTNSLKRGGAHHRRCEVALLGCGAVLAATGLGFDLLEAGKCQLLPLEEPEPPAREEKKRREGLFQRSSRPRRSTSPPSRKLFKKEEPMLLLGDPSASLTLLSLSSISECNSTRSLLRSDSDEIVVYEMPVSPVEAPPLSPCTHNPLVNVRVERFKRDPNQSLTPTHVTLTTPSQPSSHRRTPSDGALKPETLLASRSPSSNGLSPSPGAGMLKTPSPSRDPGEFPRLPDPNVVFPPTPRRWNTQQDSTLERPKTLEFLPRPRPSANRQRLDPWWFVSPSHARSTSPANSSSTETPSNLDSCFASSSSTVEERPGLPALLPFQAGPLPPTERTLLDLDAEGQSQDSTVPLCRAELNTHRPAPYEIQQEFWS

MEEEEGAVAKEWGTTPAGPVWTAVFDYEAAGDEELTLRRGDRVQVLSQDCAVSGDEGWWTGQLPSGRVGVFPSNYVAPGAPAAPAGLQLPQEIPFHELQLEEIIGVGGFGKVYRALWRGEEVAVKAARLDPEKDPAVTAEQVCQEARLFGALQHPNIIALRGACLNPPHLCLVMEYARGGALSRVLAGRRVPPHVLVNWAVQVARGMNYLHNDAPVPIIHRDLKSINILILEAIENHNLADTVLKITDFGLAREWHKTTKMSAAGTYAWMAPEVIRLSLFSKSSDVWSFGVLLWELLTGEVPYREIDALAVAYGVAMNKLTLPIPSTCPEPFARLLEECWDPDPHGRPDFGSILKRLEVIEQSALFQMPLESFHSLQEDWKLEIQHMFDDLRTKEKELRSREEELLRAAQEQRFQEEQLRRREQELAEREMDIVERELHLLMCQLSQEKPRVRKRKGNFKRSRLLKLREGGSHISLPSGFEHKITVQASPTLDKRKGSDGASPPASPSIIPRLRAIRLTPVDCGGSSSGSSSGGSGTWSRGGPPKKEELVGGKKKGRTWGPSSTLQKERVGGEERLKGLGEGSKQWSSSAPNLGKSPKHTPIAPGFASLNEMEEFAEAEDGGSSVPPSPYSTPSYLSVPLPAEPSPGARAPWEPTPSAPPARWGHGARRRCDLALLGCATLLGAVGLGADVAEARAADGEEQRRWLDGLFFPRAGRFPRGLSPPARPHGRREDVGPGLGLAPSATLVSLSSVSDCNSTRSLLRSDSDEAAPAAPSPPPSPPAPTPTPSPSTNPLVDLELESFKKDPRQSLTPTHVTAACAVSRGHRRTPSDGALGQRGPPEPAGHGPGPRDLLDFPRLPDPQALFPARRRPPEFPGRPTTLTFAPRPRPAASRPRLDPWKLVSFGRTLTISPPSRPDTPESPGPPSVQPTLLDMDMEGQNQDSTVPLCGAHGSH

PTMs - P80192/Q02779 As Substrate

Site PTM Type Enzyme
Y110 Phosphorylation
K193 Ubiquitination
T304 Phosphorylation P80192 (MAP3K9)
T305 Phosphorylation P80192 (MAP3K9)
S308 Phosphorylation P80192 (MAP3K9)
T312 Phosphorylation P80192 (MAP3K9)
T452 Phosphorylation
S519 Phosphorylation
S533 Phosphorylation
T535 Phosphorylation
S547 Phosphorylation
S548 Phosphorylation
S552 Phosphorylation
T554 Phosphorylation
T565 Phosphorylation
K594 Acetylation
K595 Acetylation
K607 Acetylation
S616 Phosphorylation
S652 Phosphorylation
K658 Ubiquitination
S693 Phosphorylation
S695 Phosphorylation
S807 Phosphorylation
T808 Phosphorylation
S809 Phosphorylation
S812 Phosphorylation
T915 Phosphorylation
S917 Phosphorylation
S938 Phosphorylation
T948 Phosphorylation
Site PTM Type Enzyme
S225 Phosphorylation
S446 Phosphorylation
S489 Phosphorylation
S498 Phosphorylation
S502 Phosphorylation
S506 Phosphorylation
S508 Phosphorylation
T558 Phosphorylation
S589 Phosphorylation
S722 Phosphorylation
S809 Phosphorylation
S830 Phosphorylation

PTMs - P80192/Q02779 As Enzyme

Substrate Site Source
P80192 (MAP3K9) T304 Uniprot
P80192 (MAP3K9) T305 Uniprot
P80192 (MAP3K9) S308 Uniprot
P80192 (MAP3K9) T312 Uniprot
Substrate Site Source
P13688-1 (CEACAM1) S508 Uniprot
P15923 (TCF3) S341 Uniprot
P15923 (TCF3) S352 Uniprot
P15923 (TCF3) T355 Uniprot
P15923 (TCF3) S359 Uniprot
P15923 (TCF3) S379 Uniprot
P45985 (MAP2K4) T261 Uniprot
Q92630 (DYRK2) T381 Uniprot
Q92630 (DYRK2) S449 Uniprot

Research Backgrounds

Function:

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade through the phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7 which in turn activate the JNKs. The MKK/JNK signaling pathway regulates stress response via activator protein-1 (JUN) and GATA4 transcription factors. Plays also a role in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis.

PTMs:

Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site. Autophosphorylation also occurs on Thr-304 and Ser-308.

Tissue Specificity:

Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Function:

Activates the JUN N-terminal pathway.

PTMs:

Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.

Tissue Specificity:

Expressed in brain and skeletal muscle.

Subunit Structure:

Homodimer. Interacts with SH3RF2.

Family&Domains:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Restrictive clause

 

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