Synaptophysin Antibody - #AF0257

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Product: Synaptophysin Antibody
Catalog: AF0257
Description: Rabbit polyclonal antibody to Synaptophysin
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog, Xenopus
Mol.Wt.: 38kDa; 34kD(Calculated).
Uniprot: P08247
RRID: AB_2833432

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(92%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
Synaptophysin Antibody detects endogenous levels of total Synaptophysin.
RRID:
AB_2833432
Cite Format: Affinity Biosciences Cat# AF0257, RRID:AB_2833432.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Major synaptic vesicle protein p38; MRX96; MRXSYP; Syn p38; Synaptophysin; Syp; SYPH; SYPH_HUMAN; SypI;

Immunogens

Immunogen:
Uniprot:

P08247(SYPH_HUMAN) >>Visit HPA database.

Gene(ID):
SYP(6855)
Expression:
P08247 SYPH_HUMAN:

Expressed in the brain, with expression in the hippocampus, the neuropil in the dentate gyrus, where expression is higher in the outer half of the molecular layer than in the inner half, and in the neuropil of CA4 and CA3.

Description:
SYP Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity. Belongs to the synaptophysin/synaptobrevin family. Homohexamer or homotetramer. Note: This description may include information from UniProtKB.
Sequence:
MLLLADMDVVNQLVAGGQFRVVKEPLGFVKVLQWVFAIFAFATCGSYSGELQLSVDCANKTESDLSIEVEFEYPFRLHQVYFDAPTCRGGTTKVFLVGDYSSSAEFFVTVAVFAFLYSMGALATYIFLQNKYRENNKGPMLDFLATAVFAFMWLVSSSAWAKGLSDVKMATDPENIIKEMPVCRQTGNTCKELRDPVTSGLNTSVVFGFLNLVLWVGNLWFVFKETGWAAPFLRAPPGAPEKQPAPGDAYGDAGYGQGPGGYGPQDSYGPQGGYQPDYGQPAGSGGSGYGPQGDYGQQGYGPQGAPTSFSNQM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
92
Xenopus
92
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P08247 As Substrate

Site PTM Type Enzyme
Y73 Phosphorylation
Y81 Phosphorylation
Y250 Phosphorylation

Research Backgrounds

Function:

Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).

PTMs:

Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.

Subcellular Location:

Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle membrane>Multi-pass membrane protein. Cell junction>Synapse>Synaptosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the brain, with expression in the hippocampus, the neuropil in the dentate gyrus, where expression is higher in the outer half of the molecular layer than in the inner half, and in the neuropil of CA4 and CA3.

Subunit Structure:

Homohexamer or homotetramer. Interacts with SRCIN1. Interacts with VAMP2; the interaction is inhibit by interaction of VAPM2 with SEPT8 (By similarity).

Family&Domains:

The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.

Belongs to the synaptophysin/synaptobrevin family.

References

1). Cyfluthrin exposure during pregnancy causes neurotoxicity in offspring-Ca2+ overload via IP3R-GRP75-VDAC1 pathway. Ecotoxicology and environmental safety, 2024 (PubMed: 38492481) [IF=6.8]
2). The Effect of Early Maternal Separation Combined With Adolescent Chronic Unpredictable Mild Stress on Behavior and Synaptic Plasticity in Adult Female Rats. Frontiers in Psychiatry, 2021 (PubMed: 33746787) [IF=4.7]

Application: WB    Species: Rat    Sample: Hippocampus tissue

Figure 5 Western blot analysis. The expressions of synaptic plasticity proteins were determined by western blot (A), including PSD-95 (B), GAP-43 (C), and SYN (D). The values represent the mean ± SEM, n = 5. *p < 0.05, **p < 0.01 vs. CON, #p < 0.05, ##p < 0.01 vs. MS. CON, control; MS, maternal separation; CUMS, chronic unpredictable mild stress.
3). Dihydroartemisinin Ameliorates Decreased Neuroplasticity-Associated Proteins and Excessive Neuronal Apoptosis in APP/PS1 Mice. Current Alzheimer Research, 2020 (PubMed: 33327914) [IF=2.1]
4). Emotional and Cognitive Impairment Induced by Sleep Deprivation Due to Altered Synaptic Plasticity in the Rat Insula. , 2022

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