Phospho-ROR1 (Tyr645) Antibody | Affinity Biosciences

Product:	Phospho-ROR1 (Tyr645) Antibody
Catalog:	AF3623
Description:	Rabbit polyclonal antibody to Phospho-ROR1 (Tyr645)
Application:	ELISA(peptide)
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	104kD(Calculated).
Uniprot:	Q01973
RRID:	AB_2846937

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Phospho-ROR1 (Tyr645) Antibody detects endogenous levels of ROR1 only when phosphorylated at Tyr645.

RRID:

AB_2846937
Cite Format: Affinity Biosciences Cat# AF3623, RRID:AB_2846937.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

dJ537F10.1; Inactive tyrosine protein kinase transmembrane receptor ROR1; MGC99659; Neurotrophic tyrosine kinase; Neurotrophic tyrosine kinase receptor related 1; Neurotrophic tyrosine kinase, receptor related 1; NTRKR1; OTTHUMP00000010573; OTTHUMP00000010574; OTTMUSP00000008344; Receptor tyrosine kinase like orphan receptor 1; receptor-related 1; RGD1559469; ROR 1; ROR1; ROR1_HUMAN; RP11 24J23.1; Tyrosine kinase like orphan receptor 1; Tyrosine protein kinase transmembrane receptor ROR1; Tyrosine-protein kinase transmembrane receptor ROR1;

Immunogens

Immunogen:

A synthesized peptide derived from human ROR1 around the phosphorylation site of Tyr645.

Uniprot:

Q01973(ROR1_HUMAN) >>Visit HPA database.

Gene(ID):

ROR1(4919)

Expression:

Q01973 ROR1_HUMAN:

Expressed strongly in human heart, lung and kidney, but weakly in the CNS. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm.

Sequence:

Q01973 ROR1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MHRPRRRGTRPPLLALLAALLLAARGAAAQETELSVSAELVPTSSWNISSELNKDSYLTLDEPMNNITTSLGQTAELHCKVSGNPPPTIRWFKNDAPVVQEPRRLSFRSTIYGSRLRIRNLDTTDTGYFQCVATNGKEVVSSTGVLFVKFGPPPTASPGYSDEYEEDGFCQPYRGIACARFIGNRTVYMESLHMQGEIENQITAAFTMIGTSSHLSDKCSQFAIPSLCHYAFPYCDETSSVPKPRDLCRDECEILENVLCQTEYIFARSNPMILMRLKLPNCEDLPQPESPEAANCIRIGIPMADPINKNHKCYNSTGVDYRGTVSVTKSGRQCQPWNSQYPHTHTFTALRFPELNGGHSYCRNPGNQKEAPWCFTLDENFKSDLCDIPACDSKDSKEKNKMEILYILVPSVAIPLAIALLFFFICVCRNNQKSSSAPVQRQPKHVRGQNVEMSMLNAYKPKSKAKELPLSAVRFMEELGECAFGKIYKGHLYLPGMDHAQLVAIKTLKDYNNPQQWTEFQQEASLMAELHHPNIVCLLGAVTQEQPVCMLFEYINQGDLHEFLIMRSPHSDVGCSSDEDGTVKSSLDHGDFLHIAIQIAAGMEYLSSHFFVHKDLAARNILIGEQLHVKISDLGLSREIYSADYYRVQSKSLLPIRWMPPEAIMYGKFSSDSDIWSFGVVLWEIFSFGLQPYYGFSNQEVIEMVRKRQLLPCSEDCPPRMYSLMTECWNEIPSRRPRFKDIHVRLRSWEGLSSHTSSTTPSGGNATTQTTSLSASPVSNLSNPRYPNYMFPSQGITPQGQIAGFIGPPIPQNQRFIPINGYPIPPGYAAFPAAHYQPTGPPRVIQHCPPPKSRSPSSASGSTSTGHVTSLPSSGSNQEANIPLLPHMSIPNHPGGMGITVFGNKSQKPYKIDSKQASLLGDANIHGHTESMISAEL

PTMs - Q01973 As Substrate

Q01973

Site	PTM Type	Source
T9	Phosphorylation	Uniprot
S161	Phosphorylation	Uniprot
Y173	Phosphorylation	Uniprot
S269	Phosphorylation	Uniprot
K464	Ubiquitination	Uniprot
K466	Ubiquitination	Uniprot
K489	Ubiquitination	Uniprot
S576	Phosphorylation	Uniprot
S577	Phosphorylation	Uniprot
K614	Ubiquitination	Uniprot
K651	Ubiquitination	Uniprot
Y786	Phosphorylation	Uniprot
Y789	Phosphorylation	Uniprot
Y828	Phosphorylation	Uniprot
Y836	Phosphorylation	Uniprot

PTMs - Q01973 As Enzyme

Q01973

Substrate	Site	Source
P21860 (ERBB3)	Y1307	Uniprot

Research Backgrounds

Q01973_ROR1_HUMAN

Function:

Has very low kinase activity in vitro and is unlikely to function as a tyrosine kinase in vivo. Receptor for ligand WNT5A which activate downstream NFkB signaling pathway and may result in the inhibition of WNT3A-mediated signaling. In inner ear, crucial for spiral ganglion neurons to innervate auditory hair cells.

Subcellular Location:

Membrane>Single-pass type I membrane protein. Cell projection>Axon.

Tissue Specificity:

Family&Domains:

Belongs to the protein kinase superfamily. Tyr protein kinase family. ROR subfamily.

Restrictive clause

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For Research Use Only.
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Phospho-ROR1 (Tyr645) Antibody - #AF3623