Phospho-SLP76 (Tyr128) Antibody | Affinity Biosciences

Product:	Phospho-SLP76 (Tyr128) Antibody
Catalog:	AF3607
Description:	Rabbit polyclonal antibody to Phospho-SLP76 (Tyr128)
Application:	ELISA(peptide)
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	65~85kD; 60kD(Calculated).
Uniprot:	Q13094
RRID:	AB_2846921

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Phospho-SLP76 (Tyr128) Antibody detects endogenous levels of SLP76 only when phosphorylated at Tyr128.

RRID:

AB_2846921
Cite Format: Affinity Biosciences Cat# AF3607, RRID:AB_2846921.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

76 kDa tyrosine phosphoprotein; CG8697; LCP 2; LCP2; LCP2_HUMAN; Lymphocyte cytosolic protein 2; SH2 domain containing leukocyte protein 76 KD; SH2 domain containing leukocyte protein of 76kD; SH2 domain containing leukocyte protein of 76kDa; SH2 domain-containing leukocyte protein of 76 kDa; SLP 76; SLP 76 tyrosine phosphoprotein; SLP-76; SLP-76 tyrosine phosphoprotein; SLP76; SLP76 tyrosine phosphoprotein;

Immunogens

Immunogen:

A synthesized peptide derived from human SLP76 around the phosphorylation site of Tyr128.

Uniprot:

Q13094(LCP2_HUMAN) >>Visit HPA database.

Gene(ID):

LCP2(3937)

Expression:

Q13094 LCP2_HUMAN:

Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.

Sequence:

Q13094 LCP2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MALRNVPFRSEVLGWDPDSLADYFKKLNYKDCEKAVKKYHIDGARFLNLTENDIQKFPKLRVPILSKLSQEINKNEERRSIFTRKPQVPRFPEETESHEEDNGGWSSFEEDDYESPNDDQDGEDDGDYESPNEEEEAPVEDDADYEPPPSNDEEALQNSILPAKPFPNSNSMYIDRPPSGKTPQQPPVPPQRPMAALPPPPAGRNHSPLPPPQTNHEEPSRSRNHKTAKLPAPSIDRSTKPPLDRSLAPFDREPFTLGKKPPFSDKPSIPAGRSLGEHLPKIQKPPLPPTTERHERSSPLPGKKPPVPKHGWGPDRRENDEDDVHQRPLPQPALLPMSSNTFPSRSTKPSPMNPLPSSHMPGAFSESNSSFPQSASLPPYFSQGPSNRPPIRAEGRNFPLPLPNKPRPPSPAEEENSLNEEWYVSYITRPEAEAALRKINQDGTFLVRDSSKKTTTNPYVLMVLYKDKVYNIQIRYQKESQVYLLGTGLRGKEDFLSVSDIIDYFRKMPLLLIDGKNRGSRYQCTLTHAAGYP

PTMs - Q13094 As Substrate

Q13094

Site	PTM Type	Enzyme	Source
Y29	Phosphorylation		Uniprot
K30	Ubiquitination		Uniprot
Y39	Phosphorylation		Uniprot
S106	Phosphorylation		Uniprot
S107	Phosphorylation		Uniprot
Y113	Phosphorylation	P06239 (LCK) , P43403 (ZAP70) , P42681 (TXK) , P43405 (SYK)	Uniprot
Y128	Phosphorylation	P06239 (LCK) , P43403 (ZAP70) , P42681 (TXK) , P43405 (SYK)	Uniprot
S130	Phosphorylation		Uniprot
Y145	Phosphorylation	P43403 (ZAP70) , P06239 (LCK) , P42681 (TXK)	Uniprot
S169	Phosphorylation		Uniprot
S171	Phosphorylation		Uniprot
Y173	Phosphorylation	Q08881 (ITK)	Uniprot
S207	Phosphorylation		Uniprot
S220	Phosphorylation		Uniprot
S222	Phosphorylation		Uniprot
T227	Phosphorylation		Uniprot
S234	Phosphorylation		Uniprot
S246	Phosphorylation		Uniprot
K260	Ubiquitination		Uniprot
S264	Phosphorylation		Uniprot
S268	Phosphorylation		Uniprot
R273	Methylation		Uniprot
S274	Phosphorylation		Uniprot
S298	Phosphorylation		Uniprot
S339	Phosphorylation		Uniprot
T341	Phosphorylation		Uniprot
S344	Phosphorylation		Uniprot
S374	Phosphorylation		Uniprot
S376	Phosphorylation	Q92918 (MAP4K1)	Uniprot
R392	Methylation		Uniprot
S410	Phosphorylation		Uniprot
S417	Phosphorylation		Uniprot
Y423	Phosphorylation	P06239 (LCK) , P43403 (ZAP70)	Uniprot
S425	Phosphorylation		Uniprot
Y426	Phosphorylation	P06239 (LCK) , P43403 (ZAP70)	Uniprot
T428	Phosphorylation		Uniprot
K438	Ubiquitination		Uniprot
T454	Phosphorylation		Uniprot
T455	Phosphorylation		Uniprot
T456	Phosphorylation		Uniprot
Y459	Phosphorylation		Uniprot
Y465	Phosphorylation		Uniprot
Y470	Phosphorylation		Uniprot
Y476	Phosphorylation		Uniprot
Y483	Phosphorylation		Uniprot
K492	Ubiquitination		Uniprot
K516	Ubiquitination		Uniprot
Y522	Phosphorylation		Uniprot
Y532	Phosphorylation		Uniprot

Research Backgrounds

Q13094_LCP2_HUMAN

Function:

Involved in T-cell antigen receptor mediated signaling.

PTMs:

Phosphorylated after T-cell receptor activation by ZAP70, ITK and TXK, which leads to the up-regulation of Th1 preferred cytokine IL-2. SYK-dependent phosphorylation is required for recruitment of PI3K signaling components.

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Subunit Structure:

Interacts with SLA. Interacts with CBLB (By similarity). Interacts with GRB2. Interacts with SHB. Interacts with PRAM1. Interacts (via SH2 domain) with CD6 (via tyrosine phosphorylated C-terminus). Interacts with FYB1 and the phosphorylated form of FYB2.

Family&Domains:

The SH2 domain mediates interaction with phosphorylated CD6 (PubMed:16914752). The SH2 domain mediates interaction with SHB (PubMed:12084069).

Research Fields

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)

· Organismal Systems > Development > Osteoclast differentiation. (View pathway)

· Organismal Systems > Immune system > Platelet activation. (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Phospho-SLP76 (Tyr128) Antibody - #AF3607