Product: Thymidine Kinase 1/TK1 Antibody
Catalog: AF6068
Description: Rabbit polyclonal antibody to Thymidine Kinase 1/TK1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 25kDa; 25kD(Calculated).
Uniprot: P04183
RRID: AB_2834981

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(92%), Sheep(92%), Rabbit(92%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Thymidine Kinase 1/TK1 Antibody detects endogenous levels of total Thymidine Kinase 1/TK1.
RRID:
AB_2834981
Cite Format: Affinity Biosciences Cat# AF6068, RRID:AB_2834981.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

cytosolic; KITH_HUMAN; Thymidine kinase 1; Thymidine kinase 1 soluble; Thymidine kinase 1 soluble isoform; Thymidine kinase; Thymidine kinase cytosolic; TK 1; TK 2; TK1; Tk1a; Tk1b; TK2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
TK is a cytosolic thymidine kinase. Phosphorylated during mitosis. Its enzymatic activity is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.
Sequence:
MSCINLPTVLPGSPSKTRGQIQVILGPMFSGKSTELMRRVRRFQIAQYKCLVIKYAKDTRYSSSFCTHDRNTMEALPACLLRDVAQEALGVAVIGIDEGQFFPDIVEFCEAMANAGKTVIVAALDGTFQRKPFGAILNLVPLAESVVKLTAVCMECFREAAYTKRLGTEKEVEVIGGADKYHSVCRLCYFKKASGQPAGPDNKENCPVPGKPGEAVAARKLFAPQQILQCSPAN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Bovine
92
Sheep
92
Rabbit
92
Zebrafish
73
Xenopus
69
Horse
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P04183 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
T8 Phosphorylation
S13 Phosphorylation P06493 (CDK1) , P24941 (CDK2)
S15 Phosphorylation
K16 Ubiquitination
S30 Phosphorylation
K32 Acetylation
Y48 Phosphorylation
K49 Ubiquitination
Y55 Phosphorylation
S62 Phosphorylation
R70 Methylation
K131 Ubiquitination
K164 Acetylation
K170 Ubiquitination
K180 Ubiquitination
Y181 Phosphorylation
S183 Phosphorylation
K192 Ubiquitination
K203 Ubiquitination
K211 Ubiquitination
K220 Acetylation
K220 Ubiquitination
C230 S-Nitrosylation
S231 Phosphorylation

Research Backgrounds

PTMs:

Phosphorylated on Ser-13 in mitosis.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homotetramer.

Family&Domains:

Belongs to the thymidine kinase family.

Research Fields

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

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For Research Use Only.
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