Phospho-IRF5 (Ser446) Antibody | Affinity Biosciences

IF/ICC

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AF3599 staining A549 cells(heat shock treatment) by IF/ICC.

Product:	Phospho-IRF5 (Ser446) Antibody
Catalog:	AF3599
Description:	Rabbit polyclonal antibody to Phospho-IRF5 (Ser446)
Application:	IF/ICC
Reactivity:	Human, Mouse
Mol.Wt.:	56kD(Calculated).
Uniprot:	Q13568
RRID:	AB_2846913

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

Phospho-IRF5 (Ser446) Antibody detects endogenous levels of IRF5 only when phosphorylated at Ser446.

RRID:

AB_2846913
Cite Format: Affinity Biosciences Cat# AF3599, RRID:AB_2846913.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Interferon regulatory factor 5; Interferon regulatory factor 5 bone marrow variant; IRF 5; IRF-5; Irf5; IRF5_HUMAN; SLEB10;

Immunogens

Immunogen:

A synthesized peptide derived from human IRF5 around the phosphorylation site of Ser446.

Uniprot:

Q13568(IRF5_HUMAN) >>Visit HPA database.

Gene(ID):

IRF5(3663)

Sequence:

Q13568 IRF5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNQSIPVAPTPPRRVRLKPWLVAQVNSCQYPGLQWVNGEKKLFCIPWRHATRHGPSQDGDNTIFKAWAKETGKYTEGVDEADPAKWKANLRCALNKSRDFRLIYDGPRDMPPQPYKIYEVCSNGPAPTDSQPPEDYSFGAGEEEEEEEELQRMLPSLSLTEDVKWPPTLQPPTLRPPTLQPPTLQPPVVLGPPAPDPSPLAPPPGNPAGFRELLSEVLEPGPLPASLPPAGEQLLPDLLISPHMLPLTDLEIKFQYRGRPPRALTISNPHGCRLFYSQLEATQEQVELFGPISLEQVRFPSPEDIPSDKQRFYTNQLLDVLDRGLILQLQGQDLYAIRLCQCKVFWSGPCASAHDSCPNPIQREVKTKLFSLEHFLNELILFQKGQTNTPPPFEIFFCFGEEWPDRKPREKKLITVQVVPVAARLLLEMFSGELSWSADSIRLQISNPDLKDRMVEQFKELHHIWQSQQRLQPVAQAPPGAGLGVGQGPWPMHPAGMQ

PTMs - Q13568 As Substrate

Q13568

Site	PTM Type	Enzyme	Source
T10	Phosphorylation		Uniprot
Y136	Phosphorylation		Uniprot
S158	Phosphorylation	Q9UHD2 (TBK1)	Uniprot
S293	Phosphorylation	Q9UHD2 (TBK1)	Uniprot
S301	Phosphorylation		Uniprot
K309	Ubiquitination		Uniprot
Y313	Phosphorylation		Uniprot
Y335	Phosphorylation		Uniprot
K411	Ubiquitination		Uniprot
K412	Ubiquitination		Uniprot
S435	Phosphorylation	O43353 (RIPK2)	Uniprot
S437	Phosphorylation		Uniprot
S440	Phosphorylation		Uniprot
S446	Phosphorylation	O14920 (IKBKB) , O43353 (RIPK2)	Uniprot

Research Backgrounds

Q13568_IRF5_HUMAN

Function:

Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling.

PTMs:

Phosphorylation of serine and threonine residues in a C-terminal autoinhibitory region, stimulates dimerization, transport into the nucleus, assembly with the coactivator CBP/p300 and initiation of transcription.

'Lys-63'-linked polyubiquitination by TRAF6 is required for activation.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Shuttles between the nucleus and the cytoplasm.

Subunit Structure:

Homodimer, when phosphorylated.

Family&Domains:

Belongs to the IRF family.

Research Fields

· Organismal Systems > Immune system > Toll-like receptor signaling pathway. (View pathway)

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Phospho-IRF5 (Ser446) Antibody - #AF3599