Phospho-DYR1A (Tyr449) Antibody - #AF3598
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF3598, RRID:AB_2846912.
Fold/Unfold
;Dual specificity tyrosine phosphorylation regulated kinase; Dual specificity tyrosine (Y) phosphorylation regulated kinase 1A; Dual specificity tyrosine phosphorylation regulated kinase 1; Dual specificity tyrosine phosphorylation regulated kinase 1A; Dual specificity tyrosine-phosphorylation-regulated kinase 1A; Dual specificity YAK 1 related kinase; Dual specificity YAK1 related kinase; Dual specificity YAK1-related kinase; DYR1A_HUMAN; DYRK 1; DYRK 1A; DYRK; DYRK1; Dyrk1a; DYRKA; hMNB; HP 86; HP86; Minibrain (Drosophila) homolog; Minibrain homolog; Minibrain, Drosophila, homolog of; MNB; MNB protein kinase; Mnb protein kinase homolog hp86; MNB protein kinase, Serine/threonine-specific; MNB/DYRK protein kinase; MNBH; MRD7; OTTHUMP00000109090; OTTHUMP00000109091; OTTHUMP00000109094; OTTHUMP00000174799; Protein kinase minibrain homolog; Serine/threonine kinase MNB;
Immunogens
A synthesized peptide derived from human DYR1A around the phosphorylation site of Tyr449.
Ubiquitous. Highest levels in skeletal muscle, testis, fetal lung and fetal kidney.
- Q13627 DYR1A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MHTGGETSACKPSSVRLAPSFSFHAAGLQMAGQMPHSHQYSDRRQPNISDQQVSALSYSDQIQQPLTNQVMPDIVMLQRRMPQTFRDPATAPLRKLSVDLIKTYKHINEVYYAKKKRRHQQGQGDDSSHKKERKVYNDGYDDDNYDYIVKNGEKWMDRYEIDSLIGKGSFGQVVKAYDRVEQEWVAIKIIKNKKAFLNQAQIEVRLLELMNKHDTEMKYYIVHLKRHFMFRNHLCLVFEMLSYNLYDLLRNTNFRGVSLNLTRKFAQQMCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYRSPEVLLGMPYDLAIDMWSLGCILVEMHTGEPLFSGANEVDQMNKIVEVLGIPPAHILDQAPKARKFFEKLPDGTWNLKKTKDGKREYKPPGTRKLHNILGVETGGPGGRRAGESGHTVADYLKFKDLILRMLDYDPKTRIQPYYALQHSFFKKTADEGTNTSNSVSTSPAMEQSQSSGTTSSTSSSSGGSSGTSNSGRARSDPTHQHRHSGGHFTAAVQAMDCETHSPQVRQQFPAPLGWSGTEAPTQVTVETHPVQETTFHVAPQQNALHHHHGNSSHHHHHHHHHHHHHGQQALGNRTRPRVYNSPTNSSSTQDSMEVGHSHHSMTSLSSSTTSSSTSSSSTGNQGNQAYQNRPVAANTLDFGQNGAMDVNLTVYSNPRQETGIAGHPTYQFSANTGPAHYMTEGHLTMRQGADREESPMTGVCVQQSPVASS
PTMs - Q13627 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K11 | Ubiquitination | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| S22 | Phosphorylation | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| Y104 | Phosphorylation | Q13627 (DYRK1A) | Uniprot |
| K105 | Acetylation | Uniprot | |
| Y111 | Phosphorylation | Q13627 (DYRK1A) | Uniprot |
| Y112 | Phosphorylation | Uniprot | |
| S127 | Phosphorylation | Uniprot | |
| K134 | Ubiquitination | Uniprot | |
| Y136 | Phosphorylation | Uniprot | |
| Y140 | Phosphorylation | Q13627 (DYRK1A) | Uniprot |
| Y145 | Phosphorylation | Uniprot | |
| Y147 | Phosphorylation | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| Y159 | Phosphorylation | Q13627 (DYRK1A) | Uniprot |
| K167 | Ubiquitination | Uniprot | |
| Y177 | Phosphorylation | Q13627 (DYRK1A) | Uniprot |
| K304 | Ubiquitination | Uniprot | |
| S310 | Phosphorylation | Q13627 (DYRK1A) | Uniprot |
| Y319 | Phosphorylation | Q13627 (DYRK1A) | Uniprot |
| Y321 | Phosphorylation | Q13627 (DYRK1A) | Uniprot |
| S324 | Phosphorylation | Uniprot | |
| S329 | Phosphorylation | Uniprot | |
| T402 | Phosphorylation | Q13627 (DYRK1A) | Uniprot |
| Y449 | Phosphorylation | Q13627 (DYRK1A) | Uniprot |
| K453 | Ubiquitination | Uniprot | |
| T466 | Phosphorylation | Uniprot | |
| S529 | Phosphorylation | Q13627 (DYRK1A) | Uniprot |
| T532 | Phosphorylation | Uniprot | |
| S538 | Phosphorylation | Uniprot | |
| T543 | Phosphorylation | Uniprot | |
| T553 | Phosphorylation | Uniprot | |
| S555 | Phosphorylation | Uniprot | |
| Y705 | Phosphorylation | Uniprot | |
| S706 | Phosphorylation | Uniprot | |
| S748 | Phosphorylation | Uniprot | |
| S758 | Phosphorylation | Uniprot | |
| S762 | Phosphorylation | Uniprot |
PTMs - Q13627 As Enzyme
| Substrate | Site | Source |
|---|---|---|
| O00401 (WASL) | T199 | Uniprot |
| O00401 (WASL) | T205 | Uniprot |
| O00401 (WASL) | T262 | Uniprot |
| O43524 (FOXO3) | S330 | Uniprot |
| O43597 (SPRY2) | T75 | Uniprot |
| O75533 (SF3B1) | T273 | Uniprot |
| O75533 (SF3B1) | T434 | Uniprot |
| P01106 (MYC) | S62 | Uniprot |
| P04637 (TP53) | S15 | Uniprot |
| P10636-8 (MAPT) | S199 | Uniprot |
| P10636-8 (MAPT) | S202 | Uniprot |
| P10636-8 (MAPT) | T205 | Uniprot |
| P10636-8 (MAPT) | T212 | Uniprot |
| P10636-8 (MAPT) | T217 | Uniprot |
| P10636 (MAPT) | S369 | Uniprot |
| P10636-8 (MAPT) | S396 | Uniprot |
| P10636-8 (MAPT) | S404 | Uniprot |
| P10636-8 (MAPT) | S422 | Uniprot |
| P10636 (MAPT) | S519 | Uniprot |
| P10636 (MAPT) | T529 | Uniprot |
| P10636 (MAPT) | S721 | Uniprot |
| P16220 (CREB1) | S133 | Uniprot |
| P24385 (CCND1) | T286 | Uniprot |
| P24928 (POLR2A) | S1616 | Uniprot |
| P24928 (POLR2A) | S1619 | Uniprot |
| P37840 (SNCA) | S87 | Uniprot |
| P46821 (MAP1B) | S1400 | Uniprot |
| P49418 (AMPH) | S262 | Uniprot |
| P49418 (AMPH) | S272 | Uniprot |
| P49418 (AMPH) | S276 | Uniprot |
| P49418 (AMPH) | S285 | Uniprot |
| P49418 (AMPH) | S293 | Uniprot |
| P49418 (AMPH) | S295 | Uniprot |
| P49418 (AMPH) | T310 | Uniprot |
| P49418 (AMPH) | T312 | Uniprot |
| P49768 (PSEN1) | T354 | Uniprot |
| P53805 (RCAN1) | S167 | Uniprot |
| P53805 (RCAN1) | T247 | Uniprot |
| P55211 (CASP9) | T125 | Uniprot |
| P61764 (STXBP1) | T479 | Uniprot |
| Q05193 (DNM1) | S795 | Uniprot |
| Q05193 (DNM1) | S857 | Uniprot |
| Q07955 (SRSF1) | S227 | Uniprot |
| Q07955 (SRSF1) | S234 | Uniprot |
| Q07955 (SRSF1) | S238 | Uniprot |
| Q12778 (FOXO1) | S329 | Uniprot |
| Q13627 (DYRK1A) | Y104 | Uniprot |
| Q13627 (DYRK1A) | Y111 | Uniprot |
| Q13627 (DYRK1A) | Y140 | Uniprot |
| Q13627 (DYRK1A) | Y159 | Uniprot |
| Q13627 (DYRK1A) | Y177 | Uniprot |
| Q13627 (DYRK1A) | S310 | Uniprot |
| Q13627 (DYRK1A) | Y319 | Uniprot |
| Q13627 (DYRK1A) | Y321 | Uniprot |
| Q13627 (DYRK1A) | T402 | Uniprot |
| Q13627 (DYRK1A) | Y449 | Uniprot |
| Q13627-1 (DYRK1A) | S519 | Uniprot |
| Q13627-2 (DYRK1A) | S520 | Uniprot |
| Q13627 (DYRK1A) | S529 | Uniprot |
| Q49AN0 (CRY2) | S558 | Uniprot |
| Q52LA3 (LIN52) | S28 | Uniprot |
| Q96EB6 (SIRT1) | T530 | Uniprot |
| Q96S94 (CCNL2) | S330 | Uniprot |
| Q96S94-1 (CCNL2) | S338 | Uniprot |
| Q96S94-1 (CCNL2) | S369 | Uniprot |
Research Backgrounds
Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities. May play a role in a signaling pathway regulating nuclear functions of cell proliferation. Modulates alternative splicing by phosphorylating the splice factor SRSF6 (By similarity). Exhibits a substrate preference for proline at position P+1 and arginine at position P-3. Has pro-survival function and negatively regulates the apoptotic process. Promotes cell survival upon genotoxic stress through phosphorylation of SIRT1. This in turn inhibits TP53 activity and apoptosis (By similarity).
Autophosphorylated on numerous tyrosine residues. Can also autophosphorylate on serine and threonine residues (in vitro).
Nucleus. Nucleus speckle.
Ubiquitous. Highest levels in skeletal muscle, testis, fetal lung and fetal kidney.
Interacts RAD54L2/ARIP4 (By similarity). Interacts with CRY2 (By similarity). Interacts with RANBP9. Interacts with WDR68. Interacts with SRSF6. Interacts with SIRT1 (By similarity).
(Microbial infection) Interacts with human adenovirus 5 E1A protein.
The polyhistidine repeats act as targeting signals to nuclear speckles.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.
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