Phospho-RapGEF1 (Tyr504) Antibody - #AF3597

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Product: Phospho-RapGEF1 (Tyr504) Antibody
Catalog: AF3597
Description: Rabbit polyclonal antibody to Phospho-RapGEF1 (Tyr504)
Application: IHC
Reactivity: Human, Mouse
Mol.Wt.: 121kD(Calculated).
Uniprot: Q13905
RRID: AB_2846911

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 100ul $350 In stock
 200ul $450 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
Phospho-RapGEF1 (Tyr504) Antibody detects endogenous levels of RapGEF1 only when phosphorylated at Tyr504.
RRID:
AB_2846911
Cite Format: Affinity Biosciences Cat# AF3597, RRID:AB_2846911.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C3G; C3G protein; CRK SH3 binding GNRP; CRK SH3-binding GNRP; DKFZp781P1719; GRF 2; GRF2; Guanine nucleotide releasing factor 2 (specific for crk proto oncogene); Guanine nucleotide releasing factor 2; Guanine nucleotide-releasing factor 2; OTTHUMP00000064558; Protein C3G; Rap guanine nucleotide exchange factor (GEF) 1; Rap guanine nucleotide exchange factor 1; RAPGEF 1; RAPGEF1; RPGF1_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human RapGEF1 around the phosphorylation site of Tyr504.

Uniprot:

Q13905(RPGF1_HUMAN) >>Visit HPA database.

Gene(ID):
RAPGEF1(2889)
Expression:
Q13905 RPGF1_HUMAN:

Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver.

Sequence:
MDTDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

PTMs - Q13905 As Substrate

Site PTM Type Enzyme
S12 Phosphorylation
S23 Phosphorylation
Y61 Phosphorylation
S76 Phosphorylation
T109 Phosphorylation
K200 Ubiquitination
S214 Phosphorylation
S217 Phosphorylation
S222 Phosphorylation
S223 Phosphorylation
S253 Phosphorylation
T261 Phosphorylation
S281 Phosphorylation
S293 Phosphorylation
S296 Phosphorylation
S307 Phosphorylation
T310 Phosphorylation
S311 Phosphorylation
S313 Phosphorylation
S314 Phosphorylation
Y329 Phosphorylation
S335 Phosphorylation
S338 Phosphorylation
Y341 Phosphorylation
S345 Phosphorylation
S349 Phosphorylation
S352 Phosphorylation
S353 Phosphorylation
S358 Phosphorylation
S360 Phosphorylation
S366 Phosphorylation
S371 Phosphorylation
Y390 Phosphorylation
S423 Phosphorylation
S463 Phosphorylation
S466 Phosphorylation
Y504 Phosphorylation P00519 (ABL1) , P12931 (SRC) , P08631 (HCK)
Y561 Phosphorylation
Y570 Phosphorylation
T572 Phosphorylation
Y579 Phosphorylation
S652 Phosphorylation
K683 Ubiquitination
Y715 Phosphorylation
Y723 Phosphorylation
K808 Ubiquitination
T819 Phosphorylation
S820 Phosphorylation
K869 Ubiquitination
T950 Phosphorylation
S951 Phosphorylation
S965 Phosphorylation
S966 Phosphorylation
T1067 Phosphorylation
T1071 Phosphorylation

Research Backgrounds

Function:

Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. Plays a role in the establishment of basal endothelial barrier function. Plays a role in nerve growth factor (NGF)-induced sustained activation of Rap1 and neurite outgrowth.

PTMs:

Phosphorylation at Tyr-504 enhances activity as Rap guanine nucleotide exchange factor.

Subcellular Location:

Early endosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver.

Subunit Structure:

Interacts with CRK via its SH3-binding sites.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Human Diseases > Cancers: Specific types > Renal cell carcinoma.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

Restrictive clause

 

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For Research Use Only.
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