Phospho-Kir6.2 (Thr224) Antibody | Affinity Biosciences

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Western blot analysis of extracts from HepG2 cells(serum starvation treatment), using Phospho-Kir6.

AF3590 at 1/100 staining Rat heart tissue by IHC-P.

Product:	Phospho-Kir6.2 (Thr224) Antibody
Catalog:	AF3590
Description:	Rabbit polyclonal antibody to Phospho-Kir6.2 (Thr224)
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	44kD(Calculated).
Uniprot:	Q14654
RRID:	AB_2846904

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Phospho-Kir6.2 (Thr224) Antibody detects endogenous levels of Kir6.2 only when phosphorylated at Thr224.

RRID:

AB_2846904
Cite Format: Affinity Biosciences Cat# AF3590, RRID:AB_2846904.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ATP sensitive inward rectifier potassium channel 11; Beta cell inward rectifier subunit; BIR; HHF 2; HHF2; IKATP; Inward rectifier K(+) channel Kir6.2; Inwardly rectifying potassium channel KIR6.2; IRK 11; IRK11; KCNJ11; Kir 6.2; Kir6.2; MGC133230; PHHI; Potassium channel inwardly rectifing subfamily J member 11; Potassium channel, inwardly rectifying subfamily J member 11; Potassium inwardly rectifying channel J11; TNDM 3; TNDM3;

Immunogens

Immunogen:

A synthesized peptide derived from human Kir6.2 around the phosphorylation site of Thr224.

Uniprot:

Q14654(KCJ11_HUMAN) >>Visit HPA database.

Gene(ID):

KCNJ11(3767)

Sequence:

Q14654 KCJ11_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLSRKGIIPEEYVLTRLAEDPAEPRYRARQRRARFVSKKGNCNVAHKNIREQGRFLQDVFTTLVDLKWPHTLLIFTMSFLCSWLLFAMAWWLIAFAHGDLAPSEGTAEPCVTSIHSFSSAFLFSIEVQVTIGFGGRMVTEECPLAILILIVQNIVGLMINAIMLGCIFMKTAQAHRRAETLIFSKHAVIALRHGRLCFMLRVGDLRKSMIISATIHMQVVRKTTSPEGEVVPLHQVDIPMENGVGGNSIFLVAPLIIYHVIDANSPLYDLAPSDLHHHQDLEIIVILEGVVETTGITTQARTSYLADEILWGQRFVPIVAEEDGRYSVDYSKFGNTIKVPTPLCTARQLDEDHSLLEALTLASARGPLRKRSVPMAKAKPKFSISPDSLS

PTMs - Q14654 As Substrate

Q14654

Site	PTM Type	Enzyme	Source
T180	Phosphorylation	Q9UQM7 (CAMK2A)	Uniprot
S208	Phosphorylation		Uniprot
T224	Phosphorylation	Q9UQM7 (CAMK2A) , Q13557 (CAMK2D)	Uniprot
K332	Ubiquitination		Uniprot
T341	Phosphorylation	P28482 (MAPK1)	Uniprot
S372	Phosphorylation		Uniprot
S385	Phosphorylation	P28482 (MAPK1)	Uniprot

Research Backgrounds

Q14654_KCJ11_HUMAN

Function:

This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium (By similarity). Subunit of ATP-sensitive potassium channels (KATP). Can form cardiac and smooth muscle-type KATP channels with ABCC9. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation.

PTMs:

Phosphorylation by MAPK1 results in changes in channel gating that destabilize the closed states and reduce the ATP sensitivity.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Subunit Structure:

Interacts with ABCC8/SUR. Interacts with ABCC9/SUR2.

Family&Domains:

Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ11 subfamily.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.

· Organismal Systems > Endocrine system > Insulin secretion. (View pathway)

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Phospho-Kir6.2 (Thr224) Antibody - #AF3590