Phospho-EZH2 (Thr345) Antibody - #AF3584
Product: | Phospho-EZH2 (Thr345) Antibody |
Catalog: | AF3584 |
Description: | Rabbit polyclonal antibody to Phospho-EZH2 (Thr345) |
Application: | WB IHC IF/ICC |
Reactivity: | Human, Mouse, Rat, Monkey |
Mol.Wt.: | 85kD, 100kD; 85kD(Calculated). |
Uniprot: | Q15910 |
RRID: | AB_2846898 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF3584, RRID:AB_2846898.
Fold/Unfold
Enhancer of zeste 2; enhancer of zeste 2 polycomb repressive complex 2 subunit; Enhancer of zeste homolog 2 (Drosophila); Enhancer of zeste homolog 2; Enhancer of zeste, Drosophila, homolog 2; ENX 1; Enx 1h; ENX-1; ENX1; Enx1h; EZH 2; EZH1; EZH2; EZH2_HUMAN; EZH2b; Histone-lysine N-methyltransferase EZH2; KMT 6; KMT6; KMT6A; Lysine N-methyltransferase 6; MGC9169; WVS; WVS2;
Immunogens
A synthesized peptide derived from human EZH2 around the phosphorylation site of Thr345.
Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis.
- Q15910 EZH2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKSMFSSNRQKILERTEILNQEWKQRRIQPVHILTSVSSLRGTRECSVTSDLDFPTQVIPLKTLNAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEKYKELTEQQLPGALPPECTPNIDGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNTETALDNKPCGPQCYQHLEGAKEFAAALTAERIKTPPKRPGGRRRGRLPNNSSRPSTPTINVLESKDTDSDREAGTETGGENNDKEEEEKKDETSSSSEANSRCQTPIKMKPNIEPPENVEWSGAEASMFRVLIGTYYDNFCAIARLIGTKTCRQVYEFRVKESSIIAPAPAEDVDTPPRKKKRKHRLWAAHCRKIQLKKDGSSNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCGAADHWDSKNVSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREMEIP
PTMs - Q15910 As Substrate
Site | PTM Type | Enzyme | Source |
---|---|---|---|
K7 | Ubiquitination | Uniprot | |
S8 | Phosphorylation | Uniprot | |
K10 | Ubiquitination | Uniprot | |
S21 | Phosphorylation | P31749 (AKT1) | Uniprot |
Y23 | Phosphorylation | Uniprot | |
K39 | Ubiquitination | Uniprot | |
K61 | Ubiquitination | Uniprot | |
S75 | O-Glycosylation | Uniprot | |
S76 | O-Glycosylation | Uniprot | |
S76 | Phosphorylation | Uniprot | |
S212 | Phosphorylation | Uniprot | |
S220 | Phosphorylation | Uniprot | |
K234 | Acetylation | Uniprot | |
K234 | Ubiquitination | Uniprot | |
Y244 | Phosphorylation | Uniprot | |
T261 | Phosphorylation | Uniprot | |
S277 | Phosphorylation | Uniprot | |
T302 | Phosphorylation | Uniprot | |
T305 | Phosphorylation | Uniprot | |
T311 | Phosphorylation | Uniprot | |
K318 | Ubiquitination | Uniprot | |
T339 | Phosphorylation | Uniprot | |
T345 | Phosphorylation | P24941 (CDK2) , P06493 (CDK1) | Uniprot |
K348 | Acetylation | Uniprot | |
S362 | Phosphorylation | Uniprot | |
S363 | Phosphorylation | P49841 (GSK3B) | Uniprot |
S366 | Phosphorylation | Uniprot | |
T367 | Phosphorylation | P49841 (GSK3B) , Q16539 (MAPK14) | Uniprot |
T369 | Phosphorylation | Uniprot | |
S375 | Phosphorylation | Uniprot | |
S380 | Phosphorylation | Uniprot | |
T388 | Phosphorylation | Uniprot | |
S405 | Phosphorylation | Uniprot | |
S406 | Phosphorylation | Uniprot | |
S408 | Phosphorylation | Uniprot | |
S412 | Phosphorylation | Uniprot | |
T416 | Phosphorylation | P24941 (CDK2) | Uniprot |
T460 | Phosphorylation | Uniprot | |
K461 | Ubiquitination | Uniprot | |
K472 | Ubiquitination | Uniprot | |
S474 | Phosphorylation | Calculation | |
S475 | Phosphorylation | Calculation | |
T487 | Phosphorylation | P06493 (CDK1) | Uniprot |
K505 | Methylation | Uniprot | |
K509 | Methylation | Uniprot | |
K510 | Methylation | Uniprot | |
K569 | Ubiquitination | Uniprot | |
K597 | Ubiquitination | Uniprot | |
K602 | Ubiquitination | Uniprot | |
K629 | Ubiquitination | Uniprot | |
K634 | Ubiquitination | Uniprot | |
Y641 | Phosphorylation | O60674 (JAK2) | Uniprot |
S690 | Phosphorylation | Uniprot | |
Y696 | Phosphorylation | Uniprot | |
K713 | Ubiquitination | Uniprot | |
T718 | Phosphorylation | Uniprot | |
S729 | Phosphorylation | Uniprot | |
K735 | Methylation | Uniprot | |
K735 | Ubiquitination | Uniprot |
Research Backgrounds
Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2. Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.
Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity. Phosphorylation at Thr-345 by CDK1 and CDK2 promotes maintenance of H3K27me3 levels at EZH2-target loci, thus leading to epigenetic gene silencing.
Sumoylated.
Glycosylated: O-GlcNAcylation at Ser-75 by OGT increases stability of EZH2 and facilitates the formation of H3K27me3 by the PRC2/EED-EZH2 complex.
Nucleus.
Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis.
Binds ATRX via the SET domain (Probable). Component of the PRC2/EED-EZH2 complex, which includes EED, EZH2, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The minimum components required for methyltransferase activity of the PRC2/EED-EZH2 complex are EED, EZH2 and SUZ12. The PRC2 complex may also interact with DNMT1, DNMT3A, DNMT3B and PHF1 via the EZH2 subunit and with SIRT1 via the SUZ12 subunit. Interacts with HDAC1 and HDAC2. Interacts with PRAME. Interacts with CDYL. Interacts with CLOCK, ARNTL/BMAL1 and CRY1 (By similarity). Interacts with DNMT3L; the interaction is direct (By similarity). Interacts with EZHIP; the interaction blocks EZH2 methyltransferase activity.
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. EZ subfamily.
Research Fields
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Metabolism > Amino acid metabolism > Lysine degradation.
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