Phospho-CASC5 (Thr943/Thr1155) Antibody

Product:	Phospho-CASC5 (Thr943/Thr1155) Antibody
Catalog:	AF3567
Description:	Rabbit polyclonal antibody to Phospho-CASC5 (Thr943/Thr1155)
Application:	ELISA(peptide)
Reactivity:	Human
Mol.Wt.:	265kD(Calculated).
Uniprot:	Q8NG31
RRID:	AB_2846881

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$450	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

Phospho-CASC5 (Thr943/Thr1155) Antibody detects endogenous levels of CASC5 only when phosphorylated at Thr943/Thr1155.

RRID:

AB_2846881
Cite Format: Affinity Biosciences Cat# AF3567, RRID:AB_2846881.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

;Protein D40/AF15q14; AF15Q14; ALL1 fused gene from chromosome 15q14; Blinkin bub linking kinetochore protein; Cancer susceptibility candidate 5; Cancer susceptibility candidate gene 5 protein; Cancer/testis antigen 29; CT29; D40; hKNL 1; hSpc105; KIAA1570; Kinetochore null 1 homolog; Kinetochore null protein 1; Kinetochore scaffold 1; KNL1;

Immunogens

Immunogen:

A synthesized peptide derived from human CASC5 around the phosphorylation site of Thr943/Thr1155.

Uniprot:

Q8NG31(KNL1_HUMAN) >>Visit HPA database.

Gene(ID):

KNL1(57082)

Expression:

Q8NG31 KNL1_HUMAN:

Highly expressed in testis, where it is localized in germ cells, in particular in spermatocytes and in the pre-acrosome of round spermatids. Detected in the acrosome of ejaculated spermatozoa. Detected in adult thymus, bone marrow, colon, small intestine, appendix and placenta, and in fetal liver and thymus.

Sequence:

Q8NG31 KNL1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDGVSSEANEENDNIERPVRRRHSSILKPPRSPLQDLRGGNERVQESNALRNKKNSRRVSFADTIKVFQTESHMKIVRKSEMEGCSAMVPSQLQLLPPGFKRFSCLSLPETETGENLLLIQNKKLEDNYCEITGMNTLLSAPIHTQMQQKEFSIIEHTRERKHANDQTVIFSDENQMDLTSSHTVMITKGLLDNPISEKSTKIDTTSFLANLKLHTEDSRMKKEVNFSVDQNTSSENKIDFNDFIKRLKTGKCSAFPDVPDKENFEIPIYSKEPNSASSTHQMHVSLKEDENNSNITRLFREKDDGMNFTQCHTANIQTLIPTSSETNSRESKGNDITIYGNDFMDLTFNHTLQILPATGNFSEIENQTQNAMDVTTGYGTKASGNKTVFKSKQNTAFQDLSINSADKIHITRSHIMGAETHIVSQTCNQDARILAMTPESIYSNPSIQGCKTVFYSSCNDAMEMTKCLSNMREEKNLLKHDSNYAKMYCNPDAMSSLTEKTIYSGEENMDITKSHTVAIDNQIFKQDQSNVQIAAAPTPEKEMMLQNLMTTSEDGKMNVNCNSVPHVSKERIQQSLSNPLSISLTDRKTELLSGENMDLTESHTSNLGSQVPLAAYNLAPESTSESHSQSKSSSDECEEITKSRNEPFQRSDIIAKNSLTDTWNKDKDWVLKILPYLDKDSPQSADCNQEIATSHNIVYCGGVLDKQITNRNTVSWEQSLFSTTKPLFSSGQFSMKNHDTAISSHTVKSVLGQNSKLAEPLRKSLSNPTPDYCHDKMIICSEEEQNMDLTKSHTVVIGFGPSELQELGKTNLEHTTGQLTTMNRQIAVKVEKCGKSPIEKSGVLKSNCIMDVLEDESVQKPKFPKEKQNVKIWGRKSVGGPKIDKTIVFSEDDKNDMDITKSYTIEINHRPLLEKRDCHLVPLAGTSETILYTCRQDDMEITRSHTTALECKTVSPDEITTRPMDKTVVFVDNHVELEMTESHTVFIDYQEKERTDRPNFELSQRKSLGTPTVICTPTEESVFFPGNGESDRLVANDSQLTPLEEWSNNRGPVEVADNMELSKSATCKNIKDVQSPGFLNEPLSSKSQRRKSLKLKNDKTIVFSENHKNDMDITQSCMVEIDNESALEDKEDFHLAGASKTILYSCGQDDMEITRSHTTALECKTLLPNEIAIRPMDKTVLFTDNYSDLEVTDSHTVFIDCQATEKILEENPKFGIGKGKNLGVSFPKDNSCVQEIAEKQALAVGNKIVLHTEQKQQLFAATNRTTNEIIKFHSAAMDEKVIGKVVDQACTLEKAQVESCQLNNRDRRNVDFTSSHATAVCGSSDNYSCLPNVISCTDNLEGSAMLLCDKDEEKANYCPVQNDLAYANDFASEYYLESEGQPLSAPCPLLEKEEVIQTSTKGQLDCVITLHKDQDLIKDPRNLLANQTLVYSQDLGEMTKLNSKRVSFKLPKDQMKVYVDDIYVIPQPHFSTDQPPLPKKGQSSINKEEVILSKAGNKSLNIIENSSAPICENKPKILNSEEWFAAACKKELKENIQTTNYNTALDFHSNSDVTKQVIQTHVNAGEAPDPVITSNVPCFHSIKPNLNNLNGKTGEFLAFQTVHLPPLPEQLLELGNKAHNDMHIVQATEIHNINIISSNAKDSRDEENKKSHNGAETTSLPPKTVFKDKVRRCSLGIFLPRLPNKRNCSVTGIDDLEQIPADTTDINHLETQPVSSKDSGIGSVAGKLNLSPSQYINEENLPVYPDEINSSDSINIETEEKALIETYQKEISPYENKMGKTCNSQKRTWVQEEEDIHKEKKIRKNEIKFSDTTQDREIFDHHTEEDIDKSANSVLIKNLSRTPSSCSSSLDSIKADGTSLDFSTYRSSQMESQFLRDTICEESLREKLQDGRITIREFFILLQVHILIQKPRQSNLPGNFTVNTPPTPEDLMLSQYVYRPKIQIYREDCEARRQKIEELKLSASNQDKLLVDINKNLWEKMRHCSDKELKAFGIYLNKIKSCFTKMTKVFTHQGKVALYGKLVQSAQNEREKLQIKIDEMDKILKKIDNCLTEMETETKNLEDEEKNNPVEEWDSEMRAAEKELEQLKTEEEELQRNLLELEVQKEQTLAQIDFMQKQRNRTEELLDQLSLSEWDVVEWSDDQAVFTFVYDTIQLTITFEESVVGFPFLDKRYRKIVDVNFQSLLDEDQAPPSSLLVHKLIFQYVEEKESWKKTCTTQHQLPKMLEEFSLVVHHCRLLGEEIEYLKRWGPNYNLMNIDINNNELRLLFSSSAAFAKFEITLFLSAYYPSVPLPSTIQNHVGNTSQDDIATILSKVPLENNYLKNVVKQIYQDLFQDCHFYH

PTMs - Q8NG31 As Substrate

Q8NG31

Site	PTM Type	Enzyme	Source
S24	Phosphorylation	Q96GD4 (AURKB)	Uniprot
S25	Phosphorylation		Uniprot
K28	Ubiquitination		Uniprot
S32	Phosphorylation		Uniprot
S60	Phosphorylation	Q96GD4 (AURKB)	Uniprot
K75	Ubiquitination		Uniprot
S80	Phosphorylation		Uniprot
K124	Ubiquitination		Uniprot
Y129	Phosphorylation		Uniprot
K150	Ubiquitination		Uniprot
S153	Phosphorylation		Uniprot
K199	Ubiquitination		Uniprot
K202	Sumoylation		Uniprot
K202	Ubiquitination		Uniprot
K213	Acetylation		Uniprot
K213	Ubiquitination		Uniprot
K223	Acetylation		Uniprot
K223	Ubiquitination		Uniprot
K238	Ubiquitination		Uniprot
K246	Ubiquitination		Uniprot
K252	Sumoylation		Uniprot
K252	Ubiquitination		Uniprot
K262	Ubiquitination		Uniprot
Y270	Phosphorylation		Uniprot
K272	Ubiquitination		Uniprot
K288	Sumoylation		Uniprot
K288	Ubiquitination		Uniprot
S294	Phosphorylation		Uniprot
K303	Ubiquitination		Uniprot
K387	Ubiquitination		Uniprot
K393	Ubiquitination		Uniprot
S402	Phosphorylation		Uniprot
S405	Phosphorylation		Uniprot
K408	Ubiquitination		Uniprot
T438	Phosphorylation		Uniprot
Y443	Phosphorylation		Uniprot
S458	Phosphorylation		Uniprot
K467	Ubiquitination		Uniprot
K476	Ubiquitination		Uniprot
K480	Ubiquitination		Uniprot
K487	Ubiquitination		Uniprot
Y489	Phosphorylation		Uniprot
S497	Phosphorylation		Uniprot
T499	Phosphorylation		Uniprot
K501	Ubiquitination		Uniprot
S505	Phosphorylation		Uniprot
T513	Phosphorylation		Uniprot
K514	Ubiquitination		Uniprot
T517	Phosphorylation		Uniprot
K526	Sumoylation		Uniprot
K526	Ubiquitination		Uniprot
T539	Phosphorylation		Uniprot
K557	Ubiquitination		Uniprot
K570	Ubiquitination		Uniprot
S576	Phosphorylation		Uniprot
S578	Phosphorylation		Uniprot
S584	Phosphorylation		Uniprot
T586	Phosphorylation		Uniprot
K589	Ubiquitination		Uniprot
S633	Phosphorylation		Uniprot
S634	Phosphorylation		Uniprot
S635	Phosphorylation		Uniprot
T642	Phosphorylation		Uniprot
K643	Ubiquitination		Uniprot
K657	Sumoylation		Uniprot
K657	Ubiquitination		Uniprot
T663	Phosphorylation		Uniprot
K666	Ubiquitination		Uniprot
K668	Ubiquitination		Uniprot
K673	Ubiquitination		Uniprot
K680	Ubiquitination		Uniprot
S682	Phosphorylation		Uniprot
K707	Ubiquitination		Uniprot
K726	Ubiquitination		Uniprot
K737	Ubiquitination		Uniprot
K749	Ubiquitination		Uniprot
S750	Phosphorylation		Uniprot
S756	Phosphorylation		Uniprot
K757	Ubiquitination		Uniprot
K764	Ubiquitination		Uniprot
S765	Phosphorylation		Uniprot
S767	Phosphorylation		Uniprot
T770	Phosphorylation		Uniprot
Y773	Phosphorylation		Uniprot
K777	Ubiquitination		Uniprot
K810	Ubiquitination		Uniprot
K830	Acetylation		Uniprot
K830	Sumoylation		Uniprot
K830	Ubiquitination		Uniprot
S837	Phosphorylation		Uniprot
K841	Ubiquitination		Uniprot
K846	Ubiquitination		Uniprot
S847	Phosphorylation		Uniprot
S858	Phosphorylation		Uniprot
K861	Ubiquitination		Uniprot
K872	Ubiquitination		Uniprot
S878	Phosphorylation		Uniprot
K883	Ubiquitination		Uniprot
K886	Ubiquitination		Uniprot
T905	Phosphorylation		Uniprot
K916	Ubiquitination		Uniprot
T943	Phosphorylation		Uniprot
K953	Ubiquitination		Uniprot
S956	Phosphorylation		Uniprot
S1004	Phosphorylation		Uniprot
K1007	Ubiquitination		Uniprot
S1008	Phosphorylation		Uniprot
T1011	Phosphorylation		Uniprot
T1013	Phosphorylation		Uniprot
T1017	Phosphorylation		Uniprot
T1019	Phosphorylation		Uniprot
S1039	Phosphorylation		Uniprot
T1042	Phosphorylation		Uniprot
K1064	Ubiquitination		Uniprot
K1069	Ubiquitination		Uniprot
K1072	Sumoylation		Uniprot
K1072	Ubiquitination		Uniprot
S1076	Phosphorylation		Uniprot
S1085	Phosphorylation		Uniprot
S1086	Phosphorylation		Uniprot
K1087	Ubiquitination		Uniprot
S1088	Phosphorylation		Uniprot
S1093	Phosphorylation		Uniprot
K1100	Ubiquitination		Uniprot
T1155	Phosphorylation		Uniprot
K1165	Ubiquitination		Uniprot
K1214	Ubiquitination		Uniprot
K1221	Ubiquitination		Uniprot
K1229	Ubiquitination		Uniprot
S1232	Phosphorylation		Uniprot
K1240	Ubiquitination		Uniprot
K1248	Ubiquitination		Uniprot
K1256	Ubiquitination		Uniprot
T1263	Phosphorylation		Uniprot
K1272	Ubiquitination		Uniprot
K1281	Ubiquitination		Uniprot
K1285	Ubiquitination		Uniprot
K1295	Ubiquitination		Uniprot
K1393	Ubiquitination		Uniprot
K1402	Ubiquitination		Uniprot
K1413	Ubiquitination		Uniprot
K1419	Ubiquitination		Uniprot
Y1432	Phosphorylation		Uniprot
K1441	Ubiquitination		Uniprot
S1448	Phosphorylation		Uniprot
K1450	Ubiquitination		Uniprot
K1453	Ubiquitination		Uniprot
K1457	Ubiquitination		Uniprot
Y1459	Phosphorylation		Uniprot
Y1464	Phosphorylation		Uniprot
K1480	Ubiquitination		Uniprot
K1481	Ubiquitination		Uniprot
K1488	Ubiquitination		Uniprot
S1494	Phosphorylation		Uniprot
K1495	Ubiquitination		Uniprot
K1499	Ubiquitination		Uniprot
S1500	Phosphorylation		Uniprot
K1515	Sumoylation		Uniprot
K1515	Ubiquitination		Uniprot
K1517	Ubiquitination		Uniprot
K1530	Acetylation		Uniprot
K1530	Ubiquitination		Uniprot
K1534	Ubiquitination		Uniprot
K1584	Ubiquitination		Uniprot
K1642	Ubiquitination		Uniprot
S1652	Phosphorylation		Uniprot
S1660	Phosphorylation		Uniprot
K1670	Methylation		Uniprot
S1675	Phosphorylation		Uniprot
S1690	Phosphorylation		Uniprot
T1692	Phosphorylation		Uniprot
K1718	Ubiquitination		Uniprot
S1724	Phosphorylation		Uniprot
K1770	Ubiquitination		Uniprot
S1773	Phosphorylation		Uniprot
K1778	Ubiquitination		Uniprot
T1814	Phosphorylation		Uniprot
T1824	Phosphorylation		Uniprot
K1830	Ubiquitination		Uniprot
S1831	Phosphorylation		Uniprot
S1834	Phosphorylation		Uniprot
K1838	Ubiquitination		Uniprot
S1841	Phosphorylation		Uniprot
T1843	Phosphorylation		Uniprot
S1845	Phosphorylation		Uniprot
S1846	Phosphorylation		Uniprot
S1848	Phosphorylation		Uniprot
S1849	Phosphorylation		Uniprot
S1850	Phosphorylation		Uniprot
K1855	Ubiquitination		Uniprot
S1860	Phosphorylation		Uniprot
T1879	Phosphorylation		Uniprot
K1888	Ubiquitination		Uniprot
S1915	Phosphorylation		Uniprot
T1925	Phosphorylation		Uniprot
K1942	Ubiquitination		Uniprot
K1961	Ubiquitination		Uniprot
K1969	Ubiquitination		Uniprot
K1976	Ubiquitination		Uniprot
K1981	Ubiquitination		Uniprot
S1986	Phosphorylation		Uniprot
Y1996	Phosphorylation		Uniprot
K2001	Acetylation		Uniprot
K2009	Ubiquitination		Uniprot
K2016	Acetylation		Uniprot
K2016	Ubiquitination		Uniprot
K2022	Ubiquitination		Uniprot
K2033	Ubiquitination		Uniprot
K2037	Ubiquitination		Uniprot
K2043	Ubiquitination		Uniprot
K2047	Ubiquitination		Uniprot
S2076	Phosphorylation		Uniprot
K2083	Ubiquitination		Uniprot
K2089	Sumoylation		Uniprot
K2089	Ubiquitination		Uniprot
T2090	Phosphorylation		Uniprot
K2106	Ubiquitination		Uniprot
K2118	Ubiquitination		Uniprot
K2214	Ubiquitination		Uniprot
K2247	Ubiquitination		Uniprot
K2324	Ubiquitination		Uniprot
K2328	Ubiquitination		Uniprot

Research Backgrounds

Q8NG31_KNL1_HUMAN

Function:

Performs two crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Required for attachment of the kinetochores to the spindle microtubules. Directly links BUB1 and BUB1B to kinetochores. Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore.

Subcellular Location:

Nucleus. Chromosome>Centromere>Kinetochore.
Note: Weakly expressed in interphase nuclei. Expression increases from prophase to late anaphase, but greatly diminishes from the telophase and cytokinesis to early G1 phase of cell cycle.

Tissue Specificity:

Subunit Structure:

Interacts with DSN1, MIS12, BUB1, BUB1B, NSL1 and ZWINT.

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Phospho-CASC5 (Thr943/Thr1155) Antibody - #AF3567