Phospho-WAVE1 (Tyr125) Antibody | Affinity Biosciences

Product:	Phospho-WAVE1 (Tyr125) Antibody
Catalog:	AF3563
Description:	Rabbit polyclonal antibody to Phospho-WAVE1 (Tyr125)
Application:	ELISA(peptide)
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	62kD(Calculated).
Uniprot:	Q92558
RRID:	AB_2846877

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Phospho-WAVE1 (Tyr125) Antibody detects endogenous levels of WAVE1 only when phosphorylated at Tyr125.

RRID:

AB_2846877
Cite Format: Affinity Biosciences Cat# AF3563, RRID:AB_2846877.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

;WASP family, verprolin homology domain-containing protein 1; WASP family member 1; FLJ31482; homology of dictyostelium scar 1; KIAA0269; Protein WAVE-1; Protein WAVE1; scar, Dictyostelium, homology of, 1; SCAR1; Similar to a plant extensin like protein; Verprolin homology domain containing protein 1; Verprolin homology domain-containing protein 1; WAS protein family, member 1; Wasf1; WASF1_HUMAN; WASL; WASP family 1; WASP family protein member 1; WASP family, verprolin homology domain-containing protein; WAVE; WAVE1; Wiskott Aldrich syndrome protein family member 1; Wiskott-Aldrich syndrome protein family member 1;

Immunogens

Immunogen:

A synthesized peptide derived from human WAVE1 around the phosphorylation site of Tyr125.

Uniprot:

Q92558(WASF1_HUMAN) >>Visit HPA database.

Gene(ID):

WASF1(8936)

Expression:

Q92558 WASF1_HUMAN:

Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.

Sequence:

Q92558 WASF1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPLVKRNIDPRHLCHTALPRGIKNELECVTNISLANIIRQLSSLSKYAEDIFGELFNEAHSFSFRVNSLQERVDRLSVSVTQLDPKEEELSLQDITMRKAFRSSTIQDQQLFDRKTLPIPLQETYDVCEQPPPLNILTPYRDDGKEGLKFYTNPSYFFDLWKEKMLQDTEDKRKEKRKQKQKNLDRPHEPEKVPRAPHDRRREWQKLAQGPELAEDDANLLHKHIEVANGPASHFETRPQTYVDHMDGSYSLSALPFSQMSELLTRAEERVLVRPHEPPPPPPMHGAGDAKPIPTCISSATGLIENRPQSPATGRTPVFVSPTPPPPPPPLPSALSTSSLRASMTSTPPPPVPPPPPPPATALQAPAVPPPPAPLQIAPGVLHPAPPPIAPPLVQPSPPVARAAPVCETVPVHPLPQGEVQGLPPPPPPPPLPPPGIRPSSPVTVTALAHPPSGLHPTPSTAPGPHVPLMPPSPPSQVIPASEPKRHPSTLPVISDARSVLLEAIRKGIQLRKVEEQREQEAKHERIENDVATILSRRIAVEYSDSEDDSEFDEVDWLE

PTMs - Q92558 As Substrate

Q92558

Site	PTM Type	Enzyme	Source
S91	Phosphorylation		Uniprot
T96	Phosphorylation		Uniprot
Y125	Phosphorylation	P12931 (SRC)	Uniprot
Y151	Phosphorylation		Uniprot
T169	Phosphorylation		Uniprot
K182	Acetylation		Uniprot
K192	Acetylation		Uniprot
S298	Phosphorylation		Uniprot
R307	Methylation		Uniprot
S310	Phosphorylation	Q00535 (CDK5)	Uniprot
R315	Methylation		Uniprot
S321	Phosphorylation		Uniprot
T323	Phosphorylation		Uniprot
S339	Phosphorylation		Uniprot
S397	Phosphorylation	Q00535 (CDK5)	Uniprot
S441	Phosphorylation	Q00535 (CDK5)	Uniprot
S489	Phosphorylation		Uniprot
T490	Phosphorylation		Uniprot
Y543	Phosphorylation		Uniprot
S546	Phosphorylation		Uniprot
S550	Phosphorylation		Uniprot

Research Backgrounds

Q92558_WASF1_HUMAN

Function:

Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex (By similarity). As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (By similarity).

PTMs:

Phosphorylated on tyrosine residues by ABL1 and dephosphorylated by PTPN12.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cell junction>Synapse. Cell junction>Focal adhesion.
Note: Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas (PubMed:9889097). Partial translocation to focal adhesion sites might be mediated by interaction with SORBS2 (PubMed:18559503). In neurons, colocalizes with activated NTRK2 after BDNF addition in endocytic sites through the association with TMEM108 (By similarity).

Tissue Specificity:

Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.

Subunit Structure:

Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterodimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1. Binds actin and the Arp2/3 complex. Interacts with BAIAP2. Interacts with SHANK3; the interaction mediates the association of SHANK3 with the WAVE1 complex. Interacts with ABI1 (via N-terminus). Interacts with SORBS2; this interaction greatly enhances phosphorylation by ABL1 and dephosphorylation by PTPN12 and might mediate partial to focal adhesion sites.

Family&Domains:

Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.

Belongs to the SCAR/WAVE family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)

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For Research Use Only.
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Phospho-WAVE1 (Tyr125) Antibody - #AF3563