Product: Phospho-NLRP3 (Ser194) Antibody
Catalog: AF3555
Description: Rabbit polyclonal antibody to Phospho-NLRP3 (Ser194)
Application: WB
Reactivity: Mouse
Mol.Wt.: 80~120kDa;
Uniprot: Q8R4B8
RRID: AB_2846869

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Mouse
Clonality:
Polyclonal
Specificity:
Phospho-NLRP3 (Ser194) Antibody detects endogenous levels of NLRP3 only when phosphorylated at Ser194.
RRID:
AB_2846869
Cite Format: Affinity Biosciences Cat# AF3555, RRID:AB_2846869.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AGTAVPRL; AII/AVP; Angiotensin/vasopressin receptor AII/AVP like; Angiotensin/vasopressin receptor AII/AVP-like; C1orf7; Caterpiller protein 1.1; CIAS 1; CIAS1; CLR1.1; Cold autoinflammatory syndrome 1; Cold autoinflammatory syndrome 1 protein; Cryopyrin; Familial cold autoinflammatory syndrome; FCAS; FCU; LRR and PYD domains-containing protein 3; Muckle-Wells syndrome; MWS; NACHT; NACHT LRR and PYD containing protein 3; NALP 3; NALP3; NALP3_HUMAN; NLR family pyrin domain containing 3; NLRP3; PYPAF 1; PYPAF1; PYRIN containing APAF1 like protein 1; PYRIN-containing APAF1-like protein 1;

Immunogens

Immunogen:

A synthesized peptide derived from mouse NLRP3 around the phosphorylation site of Ser194.

References

1). Overproduction of Gastrointestinal 5-HT Promotes Colitis-Associated Colorectal Cancer Progression via Enhancing NLRP3 Inflammasome Activation. Cancer Immunology Research, 2021 (PubMed: 34285037) [IF=10.1]

Application: WB    Species: Human    Sample: THP-1 cells

Figure 4.5-HT/HTR3A enhances NLRP3 inflammasome activation by facilitating inflammasome assembling. A–C, mRNA expression of NLRP3, IL1B, and IL18 detected by real-time PCR in THP-1 cells after activation with LPS (100 ng/mL, 4 hours), ATP (5 mmol/L, 1 hour) with/without TPS (100 μmol/L), or 2Met5HT (100 nmol/L) for 5 hours. D, Coimmunoprecipitation of NLRP3 inflammasome components [pro–caspase-1 (P-casp1), NLRP3, and ASC] with anti-ASC from lysates. G, ASC oligomerization analysis for THP-1 cells (with/without anti-HTR3A shRNA transfection) after activation with LPS (100 ng/mL, 4 hours) and ATP (5 mmol/L, 1 hour) with exogenous 5-HT (10 μmol/L, 5 hours) treatment. Coimmunoprecipitation of NLRP3 inflammasome components (P-casp1, NLRP3, and ASC) with anti-ASC from lysates (E) and ASC oligomerization analysis of THP-1 cells (H) after activation with LPS (100 ng/mL, 4 hours) and ATP (5 mmol/L, 1 hour) with/without exogenous 5-HT (10 μmol/L) and TPS (100 μmol/L) treatments for 5 hours. Coimmunoprecipitation of NLRP3 inflammasome components (P-casp1, NLRP3, and ASC) with anti-ASC from lysates (F) and ASC oligomerization analysis of THP-1 cells (I) after activation with LPS (100 ng/mL, 4 hours) and ATP (5 mmol/L, 1 hour) with/without 2Met5HT (100 nmol/L) treatment for 5 hours. J, Representative photos of ASC speck staining in THP-1 and iBMDM cells after activation with LPS (100 ng/mL, 4 hours) and ATP (5 mmol/L, 1 hour) with/without 5-HT(10 μmol/L) plus TPS (100 μmol/L) or 2Met5HT (100 nmol/L) treatments for 5 hours. A–C, Data represent mean ± SD for at least three independent experiments; one-way ANOVA. **, P < 0.01. n.s., no significant differences. Ctrl, control.

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