Phospho-NIK (Thr559) Antibody - #AF3552

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Product: Phospho-NIK (Thr559) Antibody
Catalog: AF3552
Description: Rabbit polyclonal antibody to Phospho-NIK (Thr559)
Application: WB
Reactivity: Human, Mouse, Rat
Mol.Wt.: 104kD(Calculated).
Uniprot: Q99558
RRID: AB_2846866

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MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-NIK (Thr559) Antibody detects endogenous levels of NIK only when phosphorylated at Thr559.
RRID:
AB_2846866
Cite Format: Affinity Biosciences Cat# AF3552, RRID:AB_2846866.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

;MAP3K14; FTDCR1B; HS; HsNIK; M3K14_HUMAN; Map3k14; Mitogen activated protein kinase kinase kinase 14; Mitogen-activated protein kinase kinase kinase 14; NF kappa beta inducing kinase; NF-kappa-beta-inducing kinase; NIK; Serine/threonine-protein kinase NIK;

Immunogens

Immunogen:

A synthesized peptide derived from human NIK around the phosphorylation site of Thr559.

Uniprot:

Q99558(M3K14_HUMAN) >>Visit HPA database.

Gene(ID):
MAP3K14(9020)
Expression:
Q99558 M3K14_HUMAN:

Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon.

Sequence:
MAVMEMACPGAPGSAVGQQKELPKAKEKTPPLGKKQSSVYKLEAVEKSPVFCGKWEILNDVITKGTAKEGSEAGPAAISIIAQAECENSQEFSPTFSERIFIAGSKQYSQSESLDQIPNNVAHATEGKMARVCWKGKRRSKARKKRKKKSSKSLAHAGVALAKPLPRTPEQESCTIPVQEDESPLGAPYVRNTPQFTKPLKEPGLGQLCFKQLGEGLRPALPRSELHKLISPLQCLNHVWKLHHPQDGGPLPLPTHPFPYSRLPHPFPFHPLQPWKPHPLESFLGKLACVDSQKPLPDPHLSKLACVDSPKPLPGPHLEPSCLSRGAHEKFSVEEYLVHALQGSVSSGQAHSLTSLAKTWAARGSRSREPSPKTEDNEGVLLTEKLKPVDYEYREEVHWATHQLRLGRGSFGEVHRMEDKQTGFQCAVKKVRLEVFRAEELMACAGLTSPRIVPLYGAVREGPWVNIFMELLEGGSLGQLVKEQGCLPEDRALYYLGQALEGLEYLHSRRILHGDVKADNVLLSSDGSHAALCDFGHAVCLQPDGLGKSLLTGDYIPGTETHMAPEVVLGRSCDAKVDVWSSCCMMLHMLNGCHPWTQFFRGPLCLKIASEPPPVREIPPSCAPLTAQAIQEGLRKEPIHRVSAAELGGKVNRALQQVGGLKSPWRGEYKEPRHPPPNQANYHQTLHAQPRELSPRAPGPRPAEETTGRAPKLQPPLPPEPPEPNKSPPLTLSKEESGMWEPLPLSSLEPAPARNPSSPERKATVPEQELQQLEIELFLNSLSQPFSLEEQEQILSCLSIDSLSLSDDSEKNPSKASQSSRDTLSSGVHSWSSQAEARSSSWNMVLARGRPTDTPSYFNGVKVQIQSLNGEHLHIREFHRVKVGDIATGISSQIPAAAFSLVTKDGQPVRYDMEVPDSGIDLQCTLAPDGSFAWSWRVKHGQLENRP

PTMs - Q99558 As Substrate

Site PTM Type Enzyme
Phosphorylation
Ubiquitination
Y40 Phosphorylation
K41 Ubiquitination
K47 Ubiquitination
K64 Ubiquitination
K106 Ubiquitination
T125 Phosphorylation
K128 Ubiquitination
K152 Ubiquitination
K163 Ubiquitination
K198 Ubiquitination
K201 Ubiquitination
K211 Ubiquitination
K241 Ubiquitination
S261 Phosphorylation
K276 Ubiquitination
K294 Ubiquitination
S352 Phosphorylation
T354 Phosphorylation
K373 Ubiquitination
K385 Ubiquitination
K387 Ubiquitination
S410 Phosphorylation
K429 Ubiquitination
T559 Phosphorylation O15111 (CHUK) , P41279 (MAP3K8)
S643 Phosphorylation
K650 Ubiquitination
Y682 Phosphorylation
S814 Phosphorylation
S817 Phosphorylation
S820 Phosphorylation
K882 Ubiquitination

PTMs - Q99558 As Enzyme

Substrate Site Source
O14920-1 (IKBKB) S177 Uniprot
O14920-1 (IKBKB) S181 Uniprot
O15111 (CHUK) S176 Uniprot
O15111 (CHUK) S180 Uniprot
P25963 (NFKBIA) S32 Uniprot
P25963 (NFKBIA) S36 Uniprot
P61160 (ACTR2) Y202 Uniprot
P61160 (ACTR2) T237 Uniprot
P61160 (ACTR2) T238 Uniprot
Q00653-4 (NFKB2) S865 Uniprot
Q00653 (NFKB2) S866 Uniprot
Q00653-4 (NFKB2) S869 Uniprot
Q00653 (NFKB2) S870 Uniprot

Research Backgrounds

Function:

Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner.

PTMs:

Autophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization.

Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon.

Subunit Structure:

Interacts with TRAF2, TRAF5, TRAF6, IKKA and NFKB2/P100 (By similarity). Interacts with TRAF3 and PELI3. Interacts with NIBP; the interaction is direct. Interacts with ARRB1 and ARRB2. Interacts with GRB10. Interacts with ZFP91. Interacts with NLRP12; this interaction promotes proteasomal degradation of MAP3K14.

Family&Domains:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Intestinal immune network for IgA production.   (View pathway)

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