Phospho-MAP3K3 (Ser166) Antibody - #AF3545

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Product: Phospho-MAP3K3 (Ser166) Antibody
Catalog: AF3545
Description: Rabbit polyclonal antibody to Phospho-MAP3K3 (Ser166)
Application: IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 71kD(Calculated).
Uniprot: Q99759
RRID: AB_2846859

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 100ul $350 In stock
 200ul $450 In stock

Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-MAP3K3 (Ser166) Antibody detects endogenous levels of MAP3K3 only when phosphorylated at Ser166.
RRID:
AB_2846859
Cite Format: Affinity Biosciences Cat# AF3545, RRID:AB_2846859.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

M3K3_HUMAN; MAP/ERK kinase kinase 3; MAP3K3; MAPK/ERK kinase kinase 3; MAPKKK3; MEK kinase 3; MEKK 3; MEKK3; Mitogen activated protein kinase kinase kinase 3; Mitogen-activated protein kinase kinase kinase 3;

Immunogens

Immunogen:

A synthesized peptide derived from human MAP3K3 around the phosphorylation site of Ser166.

Uniprot:

Q99759(M3K3_HUMAN) >>Visit HPA database.

Gene(ID):
MAP3K3(4215)
Sequence:
MDEQEALNSIMNDLVALQMNRRHRMPGYETMKNKDTGHSNRQSDVRIKFEHNGERRIIAFSRPVKYEDVEHKVTTVFGQPLDLHYMNNELSILLKNQDDLDKAIDILDRSSSMKSLRILLLSQDRNHNSSSPHSGVSRQVRIKASQSAGDINTIYQPPEPRSRHLSVSSQNPGRSSPPPGYVPERQQHIARQGSYTSINSEGEFIPETSEQCMLDPLSSAENSLSGSCQSLDRSADSPSFRKSRMSRAQSFPDNRQEYSDRETQLYDKGVKGGTYPRRYHVSVHHKDYSDGRRTFPRIRRHQGNLFTLVPSSRSLSTNGENMGLAVQYLDPRGRLRSADSENALSVQERNVPTKSPSAPINWRRGKLLGQGAFGRVYLCYDVDTGRELASKQVQFDPDSPETSKEVSALECEIQLLKNLQHERIVQYYGCLRDRAEKTLTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIVHRDIKGANILRDSAGNVKLGDFGASKRLQTICMSGTGMRSVTGTPYWMSPEVISGEGYGRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQPTNPQLPSHISEHGRDFLRRIFVEARQRPSAEELLTHHFAQLMY

PTMs - Q99759 As Substrate

Site PTM Type Enzyme
K102 Ubiquitination
S130 Phosphorylation
S131 Phosphorylation
S145 Phosphorylation
S147 Phosphorylation
Y155 Phosphorylation
S162 Phosphorylation
S166 Phosphorylation O00141 (SGK1)
S168 Phosphorylation
S169 Phosphorylation
S175 Phosphorylation
S176 Phosphorylation
S194 Phosphorylation
S234 Phosphorylation
S237 Phosphorylation
S250 Phosphorylation
T263 Phosphorylation
Y266 Phosphorylation
T274 Phosphorylation
Y275 Phosphorylation
Y288 Phosphorylation
S289 Phosphorylation
T294 Phosphorylation
S312 Phosphorylation
S314 Phosphorylation
S316 Phosphorylation
T317 Phosphorylation
S337 Phosphorylation O00141 (SGK1)
S340 Phosphorylation
S345 Phosphorylation
S355 Phosphorylation
S357 Phosphorylation
K366 Ubiquitination
K391 Ubiquitination
K404 Ubiquitination
K417 Ubiquitination
K456 Ubiquitination
S464 Phosphorylation
K491 Ubiquitination
K504 Ubiquitination
K512 Ubiquitination
T516 Phosphorylation
S520 Phosphorylation
S526 Phosphorylation Q99759 (MAP3K3)
T528 Phosphorylation
T530 Phosphorylation

PTMs - Q99759 As Enzyme

Substrate Site Source
P46734 (MAP2K3) S218 Uniprot
P53805 (RCAN1) S163 Uniprot
P53805 (RCAN1) S167 Uniprot
Q99759-1 (MAP3K3) S526 Uniprot
Q99759-2 (MAP3K3) S557 Uniprot

Research Backgrounds

Function:

Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.

PTMs:

Phosphorylation at Ser-166 and Ser-337 by SGK1 inhibits its activity.

Subunit Structure:

Binds both upstream activators and downstream substrates in multimolecular complexes. Part of a complex with MAP2K3, RAC1 and CCM2. Interacts with MAP2K5 and SPAG9.

Family&Domains:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

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For Research Use Only.
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