Phospho-SIK2 (Ser358) Antibody - #AF3539

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Product: Phospho-SIK2 (Ser358) Antibody
Catalog: AF3539
Description: Rabbit polyclonal antibody to Phospho-SIK2 (Ser358)
Application: IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 100~130kD; 104kD(Calculated).
Uniprot: Q9H0K1
RRID: AB_2846853

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 100ul $350 In stock
 200ul $450 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-SIK2 (Ser358) Antibody detects endogenous levels of SIK2 only when phosphorylated at Ser358.
RRID:
AB_2846853
Cite Format: Affinity Biosciences Cat# AF3539, RRID:AB_2846853.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp434K1115; EC 2.7.11.1; KIAA0781; LOH11CR1I; QIK; Qin induced kinase; Qin-induced kinase; Salt inducible kinase 2; Salt inducible protein kinase 2; Salt inducible serine/threonine kinase 2; Salt-inducible protein kinase 2; Serine/threonine protein kinase SIK2; Serine/threonine protein kinase SNF1 like kinase 2; Serine/threonine-protein kinase SIK2; Serine/threonine-protein kinase SNF1-like kinase 2; SIK 2; SIK-2; SIK2; SIK2_HUMAN; SNF1 like kinase; SNF1 like kinase 2; SNF1LK2;

Immunogens

Immunogen:

A synthesized peptide derived from human SIK2 around the phosphorylation site of Ser358.

Uniprot:

Q9H0K1(SIK2_HUMAN) >>Visit HPA database.

Gene(ID):
SIK2(23235)
Sequence:
MVMADGPRHLQRGPVRVGFYDIEGTLGKGNFAVVKLGRHRITKTEVAIKIIDKSQLDAVNLEKIYREVQIMKMLDHPHIIKLYQVMETKSMLYLVTEYAKNGEIFDYLANHGRLNESEARRKFWQILSAVDYCHGRKIVHRDLKAENLLLDNNMNIKIADFGFGNFFKSGELLATWCGSPPYAAPEVFEGQQYEGPQLDIWSMGVVLYVLVCGALPFDGPTLPILRQRVLEGRFRIPYFMSEDCEHLIRRMLVLDPSKRLTIAQIKEHKWMLIEVPVQRPVLYPQEQENEPSIGEFNEQVLRLMHSLGIDQQKTIESLQNKSYNHFAAIYFLLVERLKSHRSSFPVEQRLDGRQRRPSTIAEQTVAKAQTVGLPVTMHSPNMRLLRSALLPQASNVEAFSFPASGCQAEAAFMEEECVDTPKVNGCLLDPVPPVLVRKGCQSLPSNMMETSIDEGLETEGEAEEDPAHAFEAFQSTRSGQRRHTLSEVTNQLVVMPGAGKIFSMNDSPSLDSVDSEYDMGSVQRDLNFLEDNPSLKDIMLANQPSPRMTSPFISLRPTNPAMQALSSQKREVHNRSPVSFREGRRASDTSLTQGIVAFRQHLQNLARTKGILELNKVQLLYEQIGPEADPNLAPAAPQLQDLASSCPQEEVSQQQESVSTLPASVHPQLSPRQSLETQYLQHRLQKPSLLSKAQNTCQLYCKEPPRSLEQQLQEHRLQQKRLFLQKQSQLQAYFNQMQIAESSYPQPSQQLPLPRQETPPPSQQAPPFSLTQPLSPVLEPSSEQMQYSPFLSQYQEMQLQPLPSTSGPRAAPPLPTQLQQQQPPPPPPPPPPRQPGAAPAPLQFSYQTCELPSAASPAPDYPTPCQYPVDGAQQSDLTGPDCPRSPGLQEAPSSYDPLALSELPGLFDCEMLDAVDPQHNGYVLVN

PTMs - Q9H0K1 As Substrate

Site PTM Type Enzyme
T25 Phosphorylation
K28 Ubiquitination
K43 Ubiquitination
K49 Ubiquitination
K53 Acetylation
K53 Ubiquitination
K63 Ubiquitination
K81 Ubiquitination
S90 Phosphorylation
K144 Ubiquitination
K168 Sumoylation
T175 Phosphorylation Q15831 (STK11)
K266 Ubiquitination
K313 Ubiquitination
S342 Phosphorylation
S343 Phosphorylation
S358 Phosphorylation P31751 (AKT2) , P17612 (PRKACA)
T359 Phosphorylation
S379 Phosphorylation
T484 Phosphorylation
S486 Phosphorylation
S512 Phosphorylation
S534 Phosphorylation
K536 Ubiquitination
S545 Phosphorylation
R547 Methylation
S576 Phosphorylation
S587 Phosphorylation
T589 Phosphorylation
S590 Phosphorylation
K686 Ubiquitination
K692 Ubiquitination
Y700 Phosphorylation
K702 Ubiquitination

PTMs - Q9H0K1 As Enzyme

Substrate Site Source
P35568 (IRS1) S794 Uniprot
Q15078 (CDK5R1) S91 Uniprot
Q53ET0 (CRTC2) S171 Uniprot
Q9BV73 (CEP250) S2392 Uniprot
Q9BV73 (CEP250) S2394 Uniprot

Research Backgrounds

Function:

Phosphorylates 'Ser-794' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and repressing TORCs, the CREB-specific coactivators.

PTMs:

Phosphorylated at Thr-175 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39.

Acetylation at Lys-53 inhibits kinase activity. Deacetylated by HDAC6.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with and phosphorylates TORC2/CRTC2.

Family&Domains:

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.

Research Fields

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

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