Phospho-SENP2 (Thr368) Antibody | Affinity Biosciences

IF/ICC

1/1

AF3534 staining A549 cells(H2O2 treatment) by IF/ICC.

Product:	Phospho-SENP2 (Thr368) Antibody
Catalog:	AF3534
Description:	Rabbit polyclonal antibody to Phospho-SENP2 (Thr368)
Application:	IF/ICC
Reactivity:	Human
Mol.Wt.:	60-70kD; 68kD(Calculated).
Uniprot:	Q9HC62
RRID:	AB_2846848

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

Phospho-SENP2 (Thr368) Antibody detects endogenous levels of SENP2 only when phosphorylated at Thr368.

RRID:

AB_2846848
Cite Format: Affinity Biosciences Cat# AF3534, RRID:AB_2846848.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Axam2; DKFZp762A2316; KIAA1331; Senp2; SENP2_HUMAN; Sentrin specific protease 2; Sentrin-specific protease 2; Sentrin/SUMO specific protease SENP2; Sentrin/SUMO-specific protease SENP2; SMT3 specific isopeptidase 2; SMT3-specific isopeptidase 2; Smt3ip2; SUMO1/sentrin/SMT3 specific peptidase 2;

Immunogens

Immunogen:

A synthesized peptide derived from human SENP2 around the phosphorylation site of Thr368.

Uniprot:

Q9HC62(SENP2_HUMAN) >>Visit HPA database.

Gene(ID):

SENP2(59343)

Sequence:

Q9HC62 SENP2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MYRWLVRILGTIFRFCDRSVPPARALLKRRRSDSTLFSTVDTDEIPAKRPRLDCFIHQVKNSLYNAASLFGFPFQLTTKPMVTSACNGTRNVAPSGEVFSNSSSCELTGSGSWNNMLKLGNKSPNGISDYPKIRVTVTRDQPRRVLPSFGFTLNSEGCNRRPGGRRHSKGNPESSLMWKPQEQAVTEMISEESGKGLRRPHCTVEEGVQKEEREKYRKLLERLKESGHGNSVCPVTSNYHSSQRSQMDTLKTKGWGEEQNHGVKTTQFVPKQYRLVETRGPLCSLRSEKRCSKGKITDTETMVGIRFENESRRGYQLEPDLSEEVSARLRLGSGSNGLLRRKVSIIETKEKNCSGKERDRRTDDLLELTEDMEKEISNALGHGPQDEILSSAFKLRITRGDIQTLKNYHWLNDEVINFYMNLLVERNKKQGYPALHVFSTFFYPKLKSGGYQAVKRWTKGVNLFEQEIILVPIHRKVHWSLVVIDLRKKCLKYLDSMGQKGHRICEILLQYLQDESKTKRNSDLNLLEWTHHSMKPHEIPQQLNGSDCGMFTCKYADYISRDKPITFTQHQMPLFRKKMVWEILHQQLL

PTMs - Q9HC62 As Substrate

Q9HC62

Site	PTM Type	Enzyme	Source
S32	Phosphorylation		Uniprot
S34	Phosphorylation		Uniprot
T35	Phosphorylation		Uniprot
S123	Phosphorylation		Uniprot
Y130	Phosphorylation		Uniprot
S168	Phosphorylation		Uniprot
Y216	Phosphorylation		Uniprot
K224	Ubiquitination		Uniprot
S231	Phosphorylation		Uniprot
Y239	Phosphorylation		Uniprot
K253	Acetylation		Uniprot
K264	Acetylation		Uniprot
K271	Acetylation		Uniprot
K271	Ubiquitination		Uniprot
S284	Phosphorylation		Uniprot
S287	Phosphorylation		Uniprot
S292	Phosphorylation		Uniprot
S333	Phosphorylation		Uniprot
S344	Phosphorylation	O14757 (CHEK1)	Uniprot
T369	Phosphorylation	Q15418 (RPS6KA1)	Uniprot
K455	Ubiquitination		Uniprot
Y493	Phosphorylation		Uniprot
S496	Phosphorylation		Uniprot
Y558	Phosphorylation		Uniprot

Research Backgrounds

Q9HC62_SENP2_HUMAN

Function:

Protease that catalyzes two essential functions in the SUMO pathway. The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides, SUMO1, SUMO2 and SUMO3 leading to the mature form of the proteins. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins, by cleaving an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein. May down-regulate CTNNB1 levels and thereby modulate the Wnt pathway. Deconjugates SUMO2 from MTA1. Plays a dynamic role in adipogenesis by desumoylating and promoting the stabilization of CEBPB.

PTMs:

Polyubiquitinated; which leads to proteasomal degradation.

Subcellular Location:

Nucleus>Nuclear pore complex. Nucleus membrane>Peripheral membrane protein>Nucleoplasmic side. Cytoplasm.
Note: Shuttles between cytoplasm and nucleus.

Subunit Structure:

Binds to SUMO2 and SUMO3 (By similarity). Interacts with the C-terminal domain of NUP153 via its N-terminus. Interacts with MTA1.

Family&Domains:

The N-terminus is necessary and sufficient for nuclear envelope targeting.

Belongs to the peptidase C48 family.

Research Fields

· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)

· Genetic Information Processing > Translation > RNA transport.

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For Research Use Only.
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Phospho-SENP2 (Thr368) Antibody - #AF3534