Phospho-Anillin (Ser635) Antibody - #AF3533

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Product: Phospho-Anillin (Ser635) Antibody
Catalog: AF3533
Description: Rabbit polyclonal antibody to Phospho-Anillin (Ser635)
Application: IHC
Reactivity: Human, Rat
Mol.Wt.: 120~170kD; 124kD(Calculated).
Uniprot: Q9NQW6
RRID: AB_2846847

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 100ul $350 In stock
 200ul $450 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Clonality:
Polyclonal
Specificity:
Phospho-Anillin (Ser635) Antibody detects endogenous levels of Anillin only when phosphorylated at Ser635.
RRID:
AB_2846847
Cite Format: Affinity Biosciences Cat# AF3533, RRID:AB_2846847.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Actin binding protein scraps homolog Drosophila; Actin binding protein anillin; Actin-binding protein anillin; Anillin actin binding protein; anln; ANLN_HUMAN; DKFZp779A055; FSGS8; Scra; Scraps; Scraps homolog;

Immunogens

Immunogen:

A synthesized peptide derived from human Anillin around the phosphorylation site of Ser635.

Uniprot:

Q9NQW6(ANLN_HUMAN) >>Visit HPA database.

Gene(ID):
ANLN(54443)
Expression:
Q9NQW6 ANLN_HUMAN:

Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. In the kidney, it is widely expressed in tubules, but sparsely expressed in the glomerulus (PubMed:24676636). Expression is significantly increased in renal biopsy specimens from idiopathic FSGS (PubMed:24676636). Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.

Sequence:
MDPFTEKLLERTRARRENLQRKMAERPTAAPRSMTHAKRARQPLSEASNQQPLSGGEEKSCTKPSPSKKRCSDNTEVEVSNLENKQPVESTSAKSCSPSPVSPQVQPQAADTISDSVAVPASLLGMRRGLNSRLEATAASSVKTRMQKLAEQRRRWDNDDMTDDIPESSLFSPMPSEEKAASPPRPLLSNASATPVGRRGRLANLAATICSWEDDVNHSFAKQNSVQEQPGTACLSKFSSASGASARINSSSVKQEATFCSQRDGDASLNKALSSSADDASLVNASISSSVKATSPVKSTTSITDAKSCEGQNPELLPKTPISPLKTGVSKPIVKSTLSQTVPSKGELSREICLQSQSKDKSTTPGGTGIKPFLERFGERCQEHSKESPARSTPHRTPIITPNTKAIQERLFKQDTSSSTTHLAQQLKQERQKELACLRGRFDKGNIWSAEKGGNSKSKQLETKQETHCQSTPLKKHQGVSKTQSLPVTEKVTENQIPAKNSSTEPKGFTECEMTKSSPLKITLFLEEDKSLKVTSDPKVEQKIEVIREIEMSVDDDDINSSKVINDLFSDVLEEGELDMEKSQEEMDQALAESSEEQEDALNISSMSLLAPLAQTVGVVSPESLVSTPRLELKDTSRSDESPKPGKFQRTRVPRAESGDSLGSEDRDLLYSIDAYRSQRFKETERPSIKQVIVRKEDVTSKLDEKNNAFPCQVNIKQKMQELNNEINMQQTVIYQASQALNCCVDEEHGKGSLEEAEAERLLLIATGKRTLLIDELNKLKNEGPQRKNKASPQSEFMPSKGSVTLSEIRLPLKADFVCSTVQKPDAANYYYLIILKAGAENMVATPLASTSNSLNGDALTFTTTFTLQDVSNDFEINIEVYSLVQKKDPSGLDKKKKTSKSKAITPKRLLTSITTKSNIHSSVMASPGGLSAVRTSNFALVGSYTLSLSSVGNTKFVLDKVPFLSSLEGHIYLKIKCQVNSSVEERGFLTIFEDVSGFGAWHRRWCVLSGNCISYWTYPDDEKRKNPIGRINLANCTSRQIEPANREFCARRNTFELITVRPQREDDRETLVSQCRDTLCVTKNWLSADTKEERDLWMQKLNQVLVDIRLWQPDACYKPIGKP

PTMs - Q9NQW6 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S54 Phosphorylation
S65 Phosphorylation
S67 Phosphorylation
C71 S-Nitrosylation
S72 Phosphorylation
T75 Phosphorylation
K85 Ubiquitination
S92 Phosphorylation
S95 Phosphorylation
S97 Phosphorylation
S99 Phosphorylation
S102 Phosphorylation
T112 Phosphorylation
S140 Phosphorylation
S141 Phosphorylation
K143 Ubiquitination
S168 Phosphorylation
S169 Phosphorylation
S172 Phosphorylation
S176 Phosphorylation
S182 Phosphorylation
S189 Phosphorylation
T194 Phosphorylation
S211 Phosphorylation
S219 Phosphorylation
S225 Phosphorylation
S236 Phosphorylation
K237 Ubiquitination
S245 Phosphorylation
S250 Phosphorylation
S252 Phosphorylation
K254 Sumoylation
K254 Ubiquitination
S261 Phosphorylation
S268 Phosphorylation
K271 Ubiquitination
S274 Phosphorylation
S276 Phosphorylation
S281 Phosphorylation
S289 Phosphorylation
T294 Phosphorylation
S295 Phosphorylation
S299 Phosphorylation
T300 Phosphorylation
K307 Ubiquitination
S308 Phosphorylation
C309 S-Nitrosylation
T320 Phosphorylation
S323 Phosphorylation
T327 Phosphorylation
S330 Phosphorylation
T337 Phosphorylation
S339 Phosphorylation
T341 Phosphorylation
S344 Phosphorylation
K345 Ubiquitination
S349 Phosphorylation
S356 Phosphorylation
S358 Phosphorylation
K359 Ubiquitination
S362 Phosphorylation
T363 Phosphorylation
T364 Phosphorylation
T368 Phosphorylation
K371 Acetylation
K371 Ubiquitination
S388 Phosphorylation
S392 Phosphorylation
T393 Phosphorylation
T397 Phosphorylation
T401 Phosphorylation
T404 Phosphorylation
K413 Sumoylation
K413 Ubiquitination
T416 Phosphorylation
S417 Phosphorylation
S418 Phosphorylation
S419 Phosphorylation
T421 Phosphorylation
K428 Methylation
K428 Sumoylation
K428 Ubiquitination
K433 Ubiquitination
S449 Phosphorylation
K452 Ubiquitination
K464 Sumoylation
S471 Phosphorylation
T472 Phosphorylation
K482 Ubiquitination
T483 Phosphorylation
S485 Phosphorylation
K500 Ubiquitination
T504 Phosphorylation
T515 Phosphorylation
S517 Phosphorylation
S518 Phosphorylation
T523 Phosphorylation
K530 Ubiquitination
S531 Phosphorylation
K533 Ubiquitination
S536 Phosphorylation
K543 Ubiquitination
S553 Phosphorylation
S561 Phosphorylation
S562 Phosphorylation
S570 Phosphorylation
S621 Phosphorylation
S624 Phosphorylation
T628 Phosphorylation
K634 Ubiquitination
S637 Phosphorylation
S639 Phosphorylation
S642 Phosphorylation
K647 Ubiquitination
S658 Phosphorylation
S661 Phosphorylation
S664 Phosphorylation
Y671 Phosphorylation
S672 Phosphorylation
Y676 Phosphorylation
S678 Phosphorylation
K682 Ubiquitination
S688 Phosphorylation
K690 Ubiquitination
K702 Ubiquitination
K706 Ubiquitination
K717 Ubiquitination
K719 Ubiquitination
T767 Phosphorylation
K769 Ubiquitination
T771 Phosphorylation
K779 Ubiquitination
K790 Ubiquitination
S792 Phosphorylation
S795 Phosphorylation
K801 Ubiquitination
S913 Phosphorylation
S918 Phosphorylation
S927 Phosphorylation
K956 Acetylation
T1079 Phosphorylation
K1092 Sumoylation
K1092 Ubiquitination
K1101 Ubiquitination
Y1118 Phosphorylation

Research Backgrounds

Function:

Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression. Plays a role in bleb assembly during metaphase and anaphase of mitosis. May play a significant role in podocyte cell migration.

PTMs:

Phosphorylated during mitosis.

Ubiquitinated, and this requires FZR1/CDH1.

Subcellular Location:

Nucleus. Cytoplasm>Cytoskeleton. Cytoplasm>Cell cortex. Cell projection>Bleb.
Note: Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. In the kidney, it is widely expressed in tubules, but sparsely expressed in the glomerulus. Expression is significantly increased in renal biopsy specimens from idiopathic FSGS. Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.

Subunit Structure:

Interacts with F-actin. Interacts with CD2AP. May interact with RHOA. Interacts with FZR1/CDH1 during mitotic exit.

Restrictive clause

 

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For Research Use Only.
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