Phospho-hFANCI (Ser556) Antibody - #AF3524

Product: Phospho-hFANCI (Ser556) Antibody
Catalog: AF3524
Description: Rabbit polyclonal antibody to Phospho-hFANCI (Ser556)
Application: ELISA(peptide)
Reactivity: Human
Mol.Wt.: 149kD(Calculated).
Uniprot: Q9NVI1
RRID: AB_2846838

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MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
Phospho-hFANCI (Ser556) Antibody detects endogenous levels of hFANCI only when phosphorylated at Ser556.
RRID:
AB_2846838
Cite Format: Affinity Biosciences Cat# AF3524, RRID:AB_2846838.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FANCI; FANCI gene; FANCI_HUMAN; Fanconi anemia group I protein; Fanconi anemia, complementation group I; FLJ10719; FLJ14658; KIAA1794; Protein FACI; Protein FANCI;

Immunogens

Immunogen:

A synthesized peptide derived from human hFANCI around the phosphorylation site of Ser556.

Uniprot:

Q9NVI1(FANCI_HUMAN) >>Visit HPA database.

Gene(ID):
FANCI(55215)
Sequence:
MDQKILSLAAEKTADKLQEFLQTLREGDLTNLLQNQAVKGKVAGALLRAIFKGSPCSEEAGTLRRRKIYTCCIQLVESGDLQKEIASEIIGLLMLEAHHFPGPLLVELANEFISAVREGSLVNGKSLELLPIILTALATKKENLAYGKGVLSGEECKKQLINTLCSGRWDQQYVIQLTSMFKDVPLTAEEVEFVVEKALSMFSKMNLQEIPPLVYQLLVLSSKGSRKSVLEGIIAFFSALDKQHNEEQSGDELLDVVTVPSGELRHVEGTIILHIVFAIKLDYELGRELVKHLKVGQQGDSNNNLSPFSIALLLSVTRIQRFQDQVLDLLKTSVVKSFKDLQLLQGSKFLQNLVPHRSYVSTMILEVVKNSVHSWDHVTQGLVELGFILMDSYGPKKVLDGKTIETSPSLSRMPNQHACKLGANILLETFKIHEMIRQEILEQVLNRVVTRASSPISHFLDLLSNIVMYAPLVLQSCSSKVTEAFDYLSFLPLQTVQRLLKAVQPLLKVSMSMRDCLILVLRKAMFANQLDARKSAVAGFLLLLKNFKVLGSLSSSQCSQSLSVSQVHVDVHSHYNSVANETFCLEIMDSLRRCLSQQADVRLMLYEGFYDVLRRNSQLANSVMQTLLSQLKQFYEPKPDLLPPLKLEACILTQGDKISLQEPLDYLLCCIQHCLAWYKNTVIPLQQGEEEEEEEEAFYEDLDDILESITNRMIKSELEDFELDKSADFSQSTSIGIKNNICAFLVMGVCEVLIEYNFSISSFSKNRFEDILSLFMCYKKLSDILNEKAGKAKTKMANKTSDSLLSMKFVSSLLTALFRDSIQSHQESLSVLRSSNEFMRYAVNVALQKVQQLKETGHVSGPDGQNPEKIFQNLCDITRVLLWRYTSIPTSVEESGKKEKGKSISLLCLEGLQKIFSAVQQFYQPKIQQFLRALDVTDKEGEEREDADVSVTQRTAFQIRQFQRSLLNLLSSQEEDFNSKEALLLVTVLTSLSKLLEPSSPQFVQMLSWTSKICKENSREDALFCKSLMNLLFSLHVSYKSPVILLRDLSQDIHGHLGDIDQDVEVEKTNHFAIVNLRTAAPTVCLLVLSQAEKVLEEVDWLITKLKGQVSQETLSEEASSQATLPNQPVEKAIIMQLGTLLTFFHELVQTALPSGSCVDTLLKDLCKMYTTLTALVRYYLQVCQSSGGIPKNMEKLVKLSGSHLTPLCYSFISYVQNKSKSLNYTGEKKEKPAAVATAMARVLRETKPIPNLIFAIEQYEKFLIHLSKKSKVNLMQHMKLSTSRDFKIKGNILDMVLREDGEDENEEGTASEHGGQNKEPAKKKRKK

PTMs - Q9NVI1 As Substrate

Site PTM Type Enzyme
K4 Ubiquitination
K12 Ubiquitination
K16 Acetylation
K16 Ubiquitination
K39 Ubiquitination
K41 Ubiquitination
K52 Ubiquitination
T62 Phosphorylation
K125 Ubiquitination
K141 Ubiquitination
K148 Ubiquitination
S152 Phosphorylation
K157 Ubiquitination
K158 Ubiquitination
Y215 Phosphorylation
S222 Phosphorylation
K331 Ubiquitination
K336 Ubiquitination
K339 Ubiquitination
K348 Ubiquitination
K402 Ubiquitination
T406 Phosphorylation
S407 Phosphorylation
S409 Phosphorylation
S411 Phosphorylation
K420 Ubiquitination
K431 Ubiquitination
K501 Ubiquitination
K523 Ubiquitination
S556 Phosphorylation Q13315 (ATM)
S559 Phosphorylation Q13315 (ATM)
S565 Phosphorylation
Y606 Phosphorylation
Y610 Phosphorylation
S617 Phosphorylation
K638 Ubiquitination
K646 Acetylation
T653 Phosphorylation
K715 Ubiquitination
K725 Ubiquitination
S726 Phosphorylation
S730 Phosphorylation Q13315 (ATM)
K780 Ubiquitination
S782 Phosphorylation
K788 Ubiquitination
K799 Ubiquitination
T800 Phosphorylation
K849 Ubiquitination
K854 Ubiquitination
K869 Ubiquitination
K897 Ubiquitination
K902 Ubiquitination
S903 Phosphorylation
K926 Ubiquitination
K939 Ubiquitination
T952 Phosphorylation
S972 Phosphorylation Q13315 (ATM)
S1000 Phosphorylation
K1015 Ubiquitination
S1027 Phosphorylation
K1040 Ubiquitination
K1068 Ubiquitination
K1105 Ubiquitination
K1107 Ubiquitination
S1120 Phosphorylation
S1121 Phosphorylation Q13315 (ATM)
K1196 Ubiquitination
K1199 Ubiquitination
K1221 Ubiquitination
K1229 Ubiquitination
K1232 Ubiquitination
T1238 Phosphorylation
K1248 Ubiquitination
K1272 Ubiquitination
K1280 Ubiquitination
K1290 Ubiquitination
S1312 Phosphorylation

Research Backgrounds

Function:

Plays an essential role in the repair of DNA double-strand breaks by homologous recombination and in the repair of interstrand DNA cross-links (ICLs) by promoting FANCD2 monoubiquitination by FANCL and participating in recruitment to DNA repair sites. Required for maintenance of chromosomal stability. Specifically binds branched DNA: binds both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). Participates in S phase and G2 phase checkpoint activation upon DNA damage.

PTMs:

Monoubiquitinated by FANCL on Lys-523 during S phase and upon genotoxic stress. Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex. Ubiquitination is required for binding to chromatin, DNA repair, and normal cell cycle progression. Monoubiquitination is stimulated by DNA-binding.

Phosphorylated in response to DNA damage by ATM and/or ATR.

Subcellular Location:

Nucleus.
Note: Observed in spots localized in pairs on the sister chromatids of mitotic chromosome arms and not centromeres, one on each chromatids. These foci coincide with common fragile sites. They are frequently interlinked through BLM-associated ultra-fine DNA bridges.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with FANCD2; the interaction is direct. Interacts with FANCL. Interacts with MTMR15/FAN1. Interacts with POLN.

Family&Domains:

The C-terminal 30 residues are probably required for function in DNA repair.

Research Fields

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

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