Phospho-ATG4B (Ser316) Antibody | Affinity Biosciences

Product:	Phospho-ATG4B (Ser316) Antibody
Catalog:	AF3505
Description:	Rabbit polyclonal antibody to Phospho-ATG4B (Ser316)
Application:	ELISA(peptide)
Reactivity:	Human, Mouse
Mol.Wt.:	44kD(Calculated).
Uniprot:	Q9Y4P1
RRID:	AB_2846819

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

Phospho-ATG4B (Ser316) Antibody detects endogenous levels of ATG4B only when phosphorylated at Ser316.

RRID:

AB_2846819
Cite Format: Affinity Biosciences Cat# AF3505, RRID:AB_2846819.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

APG4 autophagy 4 homolog B; APG4B; ATG4 autophagy related 4 homolog B (S. cerevisiae); ATG4B; ATG4B_HUMAN; AUT like 1 cysteine endopeptidase; AUT-like 1 cysteine endopeptidase; AUTL1; Autophagin 1; Autophagin-1; Autophagy related 4B cysteine peptidase; Autophagy related cysteine endopeptidase 1; Autophagy related protein 4 homolog B; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; Cysteine protease ATG4B; hAPG4B; MGC1353;

Immunogens

Immunogen:

A synthesized peptide derived from human ATG4B around the phosphorylation site of Ser316.

Uniprot:

Q9Y4P1(ATG4B_HUMAN) >>Visit HPA database.

Gene(ID):

ATG4B(23192)

Expression:

Q9Y4P1 ATG4B_HUMAN:

Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.

Sequence:

Q9Y4P1 ATG4B_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDAATLTYDTLRFAEFEDFPETSEPVWILGRKYSIFTEKDEILSDVASRLWFTYRKNFPAIGGTGPTSDTGWGCMLRCGQMIFAQALVCRHLGRDWRWTQRKRQPDSYFSVLNAFIDRKDSYYSIHQIAQMGVGEGKSIGQWYGPNTVAQVLKKLAVFDTWSSLAVHIAMDNTVVMEEIRRLCRTSVPCAGATAFPADSDRHCNGFPAGAEVTNRPSPWRPLVLLIPLRLGLTDINEAYVETLKHCFMMPQSLGVIGGKPNSAHYFIGYVGEELIYLDPHTTQPAVEPTDGCFIPDESFHCQHPPCRMSIAELDPSIAVGFFCKTEDDFNDWCQQVKKLSLLGGALPMFELVELQPSHLACPDVLNLSLDSSDVERLERFFDSEDEDFEILSL

PTMs - Q9Y4P1 As Substrate

Q9Y4P1

Site	PTM Type	Source
M1	Acetylation	Uniprot
Y8	Phosphorylation	Uniprot
S34	Phosphorylation	Uniprot
K39	Ubiquitination	Uniprot
S44	Phosphorylation	Uniprot
K102	Ubiquitination	Uniprot
K119	Ubiquitination	Uniprot
Y122	Phosphorylation	Uniprot
Y123	Phosphorylation	Uniprot
K153	Ubiquitination	Uniprot
S316	Phosphorylation	Uniprot
K337	Ubiquitination	Uniprot
S383	Phosphorylation	Uniprot
S392	Phosphorylation	Uniprot

Research Backgrounds

Q9Y4P1_ATG4B_HUMAN

Function:

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.

Family&Domains:

Belongs to the peptidase C54 family.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - other. (View pathway)

· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)

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For Research Use Only.
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Phospho-ATG4B (Ser316) Antibody - #AF3505