Phospho-MAP3K4 (Ser499) Antibody - #AF3502

Product: Phospho-MAP3K4 (Ser499) Antibody
Catalog: AF3502
Description: Rabbit polyclonal antibody to Phospho-MAP3K4 (Ser499)
Application: ELISA(peptide)
Reactivity: Human
Mol.Wt.: 182kD(Calculated).
Uniprot: Q9Y6R4
RRID: AB_2846816

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
Phospho-MAP3K4 (Ser499) Antibody detects endogenous levels of MAP3K4 only when phosphorylated at Ser499.
RRID:
AB_2846816
Cite Format: Affinity Biosciences Cat# AF3502, RRID:AB_2846816.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

dJ473J16.1 (mitogen-activated protein kinase kinase kinase 4); FLJ42439; KIAA0213; M3K4_HUMAN; MAP three kinase 1; MAP/ERK kinase kinase 4; MAP3K4; MAPK/ERK kinase kinase 4; MAPKKK4; MEK kinase 4; MEKK 4; MEKK4; Mitogen-activated protein kinase kinase kinase 4; MTK1; predicted protein of HQ0412; PRO0412; RP3-473J16.4; SSK2/SSK22 MAP kinase kinase kinase, yeast, homolog of;

Immunogens

Immunogen:

A synthesized peptide derived from human MAP3K4 around the phosphorylation site of Ser499.

Uniprot:

Q9Y6R4(M3K4_HUMAN) >>Visit HPA database.

Gene(ID):
MAP3K4(4216)
Expression:
Q9Y6R4 M3K4_HUMAN:

Expressed at high levels in heart, placenta, skeletal muscle and pancreas, and at lower levels in other tissues.

Sequence:
MREAAAALVPPPAFAVTPAAAMEEPPPPPPPPPPPPEPETESEPECCLAARQEGTLGDSACKSPESDLEDFSDETNTENLYGTSPPSTPRQMKRMSTKHQRNNVGRPASRSNLKEKMNAPNQPPHKDTGKTVENVEEYSYKQEKKIRAALRTTERDRKKNVQCSFMLDSVGGSLPKKSIPDVDLNKPYLSLGCSNAKLPVSVPMPIARPARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTSGFWLNRSNELIWLELQAWHAGRTINDQDFFLYTARQAIPDIINEILTFKVDYGSFAFVRDRAGFNGTSVEGQCKATPGTKIVGYSTHHEHLQRQRVSFEQVKRIMELLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKGNEPEYEGDDTEGELKELESSTDESEEEQISDPRVPEIRQPIDNSFDIQSRDCISKKLERLESEDDSLGWGAPDWSTEAGFSRHCLTSIYRPFVDKALKQMGLRKLILRLHKLMDGSLQRARIALVKNDRPVEFSEFPDPMWGSDYVQLSRTPPSSEEKCSAVSWEELKAMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLQEVLEDLEKPDCNIDAFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGSFLEFGLQESCAEFWTSADDSSASDEIRRSVIEISRALKELFHEARERASKALGFAKMLRKDLEIAAEFRLSAPVRDLLDVLKSKQYVKVQIPGLENLQMFVPDTLAEEKSIILQLLNAAAGKDCSKDSDDVLIDAYLLLTKHGDRARDSEDSWGTWEAQPVKVVPQVETVDTLRSMQVDNLLLVVMQSAHLTIQRKAFQQSIEGLMTLCQEQTSSQPVIAKALQQLKNDALELCNRISNAIDRVDHMFTSEFDAEVDESESVTLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYLAIHRNSPRPMKVPRCHSDPPNPHLIIPTPEGFSTRSMPSDARSHGSPAAAAAAAAAAVAASRPSPSGGDSVLPKSISSAHDTRGSSVPENDRLASIAAELQFRSLSRHSSPTEERDEPAYPRGDSSGSTRRSWELRTLISQSKDTASKLGPIEAIQKSVRLFEEKRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITIAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERLSPEGKDFLSHCLESDPKMRWTASQLLDHSFVKVCTDEE

PTMs - Q9Y6R4 As Substrate

Site PTM Type Enzyme
S63 Phosphorylation
S66 Phosphorylation
S72 Phosphorylation
Y81 Phosphorylation
S84 Phosphorylation
S87 Phosphorylation
K98 Acetylation
Y138 Phosphorylation
Y140 Phosphorylation
S173 Phosphorylation
S253 Phosphorylation
S431 Phosphorylation
S435 Phosphorylation
Y442 Phosphorylation
T447 Phosphorylation
S456 Phosphorylation
S457 Phosphorylation
T458 Phosphorylation
S461 Phosphorylation
S481 Phosphorylation
S499 Phosphorylation
Y582 Phosphorylation
S798 Phosphorylation
S835 Phosphorylation
K890 Acetylation
S979 Phosphorylation
S1068 Phosphorylation
Y1076 Phosphorylation
S1135 Phosphorylation
S1148 Phosphorylation
S1159 Phosphorylation
S1178 Phosphorylation
S1252 Phosphorylation
S1268 Phosphorylation
S1270 Phosphorylation
T1271 Phosphorylation
S1274 Phosphorylation
S1282 Phosphorylation
Y1405 Phosphorylation
T1494 Phosphorylation Q9Y6R4 (MAP3K4)
T1505 Phosphorylation
Y1548 Phosphorylation
Y1556 Phosphorylation
K1575 Ubiquitination
S1579 Phosphorylation
S1584 Phosphorylation

PTMs - Q9Y6R4 As Enzyme

Substrate Site Source
Q9Y6R4 (MAP3K4) T1494 Uniprot

Research Backgrounds

Function:

Component of a protein kinase signal transduction cascade. Activates the CSBP2, P38 and JNK MAPK pathways, but not the ERK pathway. Specifically phosphorylates and activates MAP2K4 and MAP2K6.

Subcellular Location:

Cytoplasm>Perinuclear region.
Note: Localized in perinuclear vesicular-like structures, probably Golgi-associated vesicles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed at high levels in heart, placenta, skeletal muscle and pancreas, and at lower levels in other tissues.

Subunit Structure:

Monomer and homodimer. Homodimerization enhances kinase activity. Interacts with TRAF4; this promotes homodimerization. Binds both upstream activators and downstream substrates in multimolecular complexes. Interacts with AXIN1 and DIXDC1; interaction with DIXDC1 prevents interaction with AXIN1. Interacts with GADD45 and MAP2K6. Interacts with ZFP36; this interaction enhances the association with SH3KBP1/CIN85. Interacts with SH3KBP1; this interaction enhances the association with ZFP36. Interacts with CDC42 (By similarity).

Family&Domains:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

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