ACSS1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from TSA treated samples, using Acetyl-ACSS1 (Lys642) Antibody.

AF1001 at 1/100 staining Mouse colorectal tissue by IHC-P.

AF1001 at 1/100 staining Rat heart tissue by IHC-P.

Product:	ACSS1 Antibody
Catalog:	AF1001
Description:	Rabbit polyclonal antibody to ACSS1
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	70 kDa; 75kD(Calculated).
Uniprot:	Q9NUB1
RRID:	AB_2846798

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

ACSS1 Antibody detects endogenous levels of total ACSS1.

RRID:

AB_2846798
Cite Format: Affinity Biosciences Cat# AF1001, RRID:AB_2846798.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ACAS2L; AceCS2; AceCS2L; Acetate CoA ligase 2; Acetate--CoA ligase 2; Acetyl CoA synthetase 2; Acetyl Coenzyme A synthetase 2 (AMP forming) like; Acetyl coenzyme A synthetase 2 like, mitochondrial; Acetyl-CoA synthetase 2; Acetyl-coenzyme A synthetase 2-like, mitochondrial; ACS2L_HUMAN; Acss1; Acyl CoA synthetase short chain family member 1; Acyl-CoA synthetase short-chain family member 1; FLJ45659; KIAA1846; MGC33843;

Immunogens

Immunogen:

A synthesized peptide derived from human ACSS1 around the acetylation site of Lys642.

Uniprot:

Q9NUB1(ACS2L_HUMAN) >>Visit HPA database.

Gene(ID):

ACSS1(84532)

Sequence:

Q9NUB1 ACS2L_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAARTLGRGVGRLLGSLRGLSGQPARPPCGVSAPRRAASGPSGSAPAVAAAAAQPGSYPALSAQAAREPAAFWGPLARDTLVWDTPYHTVWDCDFSTGKIGWFLGGQLNVSVNCLDQHVRKSPESVALIWERDEPGTEVRITYRELLETTCRLANTLKRHGVHRGDRVAIYMPVSPLAVAAMLACARIGAVHTVIFAGFSAESLAGRINDAKCKVVITFNQGLRGGRVVELKKIVDEAVKHCPTVQHVLVAHRTDNKVHMGDLDVPLEQEMAKEDPVCAPESMGSEDMLFMLYTSGSTGMPKGIVHTQAGYLLYAALTHKLVFDHQPGDIFGCVADIGWITGHSYVVYGPLCNGATSVLFESTPVYPNAGRYWETVERLKINQFYGAPTAVRLLLKYGDAWVKKYDRSSLRTLGSVGEPINCEAWEWLHRVVGDSRCTLVDTWWQTETGGICIAPRPSEEGAEILPAMAMRPFFGIVPVLMDEKGSVVEGSNVSGALCISQAWPGMARTIYGDHQRFVDAYFKAYPGYYFTGDGAYRTEGGYYQITGRMDDVINISGHRLGTAEIEDAIADHPAVPESAVIGYPHDIKGEAAFAFIVVKDSAGDSDVVVQELKSMVATKIAKYAVPDEILVVKRLPKTRSGKVMRRLLRKIITSEAQELGDTTTLEDPSIIAEILSVYQKCKDKQAAAK

PTMs - Q9NUB1 As Substrate

Q9NUB1

Site	PTM Type	Source
S16	Phosphorylation	Uniprot
S32	Phosphorylation	Uniprot
S285	Phosphorylation	Uniprot
K396	Acetylation	Uniprot
Y521	Phosphorylation	Uniprot
K523	Ubiquitination	Uniprot
Y542	Phosphorylation	Uniprot
K633	Ubiquitination	Uniprot
K642	Acetylation	Uniprot
Y678	Phosphorylation	Uniprot

Research Backgrounds

Q9NUB1_ACS2L_HUMAN

Function:

Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids. Acetate is the preferred substrate. Can also utilize propionate with a much lower affinity (By similarity). Provides acetyl-CoA that is utilized mainly for oxidation under ketogenic conditions (By similarity). Involved in thermogenesis under ketogenic conditions, using acetate as a vital fuel when carbohydrate availability is insufficient (By similarity).

PTMs:

Reversibly acetylated on Lys-642. The acetyl-CoA synthase activity is inhibited by acetylation and activated by deacetylation mediated by the deacetylase SIRT3.

Subcellular Location:

Mitochondrion matrix.

Subunit Structure:

Interacts with SIRT3.

Family&Domains:

Belongs to the ATP-dependent AMP-binding enzyme family.

Research Fields

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.

· Metabolism > Carbohydrate metabolism > Propanoate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

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For Research Use Only.
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ACSS1 Antibody - #AF1001