FANCG Antibody | Affinity Biosciences

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Figure 3 hSCAPs’ odontoblastic differentiation ability and FTO expression under different conditions.

Product:	FANCG Antibody
Catalog:	AF0006
Description:	Rabbit polyclonal antibody to FANCG
Application:	WB
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	69kDa; 69kD(Calculated).
Uniprot:	O15287
RRID:	AB_2846783

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

FANCG Antibody detects endogenous levels of total FANCG.

RRID:

AB_2846783
Cite Format: Affinity Biosciences Cat# AF0006, RRID:AB_2846783.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DNA repair protein XRCC9; FAG; FANCG; FANCG_HUMAN; Fanconi anaemia complementation group G; Fanconi anemia group G protein; Protein FACG; X ray repair, complementing defective, in Chinese hamster cells 9; X-ray repair, complementing defective, in Chinese hamster, 9; XRCC9;

Immunogens

Immunogen:

A synthesized peptide derived from human FANCG.

Uniprot:

O15287(FANCG_HUMAN) >>Visit HPA database.

Gene(ID):

FANCG(2189)

Expression:

O15287 FANCG_HUMAN:

Highly expressed in testis and thymus. Found in lymphoblasts.

Description:

FANCG DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Candidate tumor suppressor gene. Belongs to the multisubunit FA complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. The complex is not found in FA patients. Note: This description may include information from UniProtKB.

Sequence:

O15287 FANCG_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSRQTTSVGSSCLDLWREKNDRLVRQAKVAQNSGLTLRRQQLAQDALEGLRGLLHSLQGLPAAVPVLPLELTVTCNFIILRASLAQGFTEDQAQDIQRSLERVLETQEQQGPRLEQGLRELWDSVLRASCLLPELLSALHRLVGLQAALWLSADRLGDLALLLETLNGSQSGASKDLLLLLKTWSPPAEELDAPLTLQDAQGLKDVLLTAFAYRQGLQELITGNPDKALSSLHEAASGLCPRPVLVQVYTALGSCHRKMGNPQRALLYLVAALKEGSAWGPPLLEASRLYQQLGDTTAELESLELLVEALNVPCSSKAPQFLIEVELLLPPPDLASPLHCGTQSQTKHILASRCLQTGRAGDAAEHYLDLLALLLDSSEPRFSPPPSPPGPCMPEVFLEAAVALIQAGRAQDALTLCEELLSRTSSLLPKMSRLWEDARKGTKELPYCPLWVSATHLLQGQAWVQLGAQKVAISEFSRCLELLFRATPEEKEQGAAFNCEQGCKSDAALQQLRAAALISRGLEWVASGQDTKALQDFLLSVQMCPGNRDTYFHLLQTLKRLDRRDEATALWWRLEAQTKGSHEDALWSLPLYLESYLSWIRPSDRDAFLEEFRTSLPKSCDL

PTMs - O15287 As Substrate

O15287

Site	PTM Type	Enzyme	Source
S7	Phosphorylation		Uniprot
K28	Ubiquitination		Uniprot
K182	Ubiquitination		Uniprot
K258	Ubiquitination		Uniprot
K347	Ubiquitination		Uniprot
S383	Phosphorylation	P06493 (CDK1)	Uniprot
S387	Phosphorylation	P06493 (CDK1)	Uniprot
K430	Ubiquitination		Uniprot
T487	Phosphorylation	P06493 (CDK1)	Uniprot
K491	Ubiquitination		Uniprot
K504	Ubiquitination		Uniprot
T557	Phosphorylation		Uniprot
K559	Ubiquitination		Uniprot
K618	Ubiquitination		Uniprot

Research Backgrounds

O15287_FANCG_HUMAN

Function:

DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Candidate tumor suppressor gene.

Subcellular Location:

Nucleus. Cytoplasm.
Note: The major form is nuclear. The minor form is cytoplasmic.

Tissue Specificity:

Highly expressed in testis and thymus. Found in lymphoblasts.

Subunit Structure:

Belongs to the multisubunit FA complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. The complex is not found in FA patients. In complex with FANCF, FANCA and FANCL, but not with FANCC, nor FANCE, interacts with HES1; this interaction may be essential for the stability and nuclear localization of FA core complex proteins. The complex with FANCC and FANCG may also include EIF2AK2 and HSP70. When phosphorylated at Ser-7, forms a complex with BRCA2, FANCD2 and XRCC3.

Research Fields

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

References

1). TBK1 Facilitates GLUT1-Dependent Glucose Consumption by suppressing mTORC1 Signaling in Colorectal Cancer Progression. International Journal of Biological Sciences, 2023 (PubMed: 35637944) [IF=9.2]

2). Single xenotransplant of rat brown adipose tissue prolonged the ovarian lifespan of aging mice by improving follicle survival. Aging Cell, 2019 (PubMed: 31389140) [IF=7.8]

3). FTO Positively Regulates Odontoblastic Differentiation via SMOC2 in Human Stem Cells from the Apical Papilla under Inflammatory Microenvironment. International journal of molecular sciences, 2024 (PubMed: 38612855) [IF=5.6]

Application: WB Species: Human Sample: hSCAPs

Figure 3 hSCAPs’ odontoblastic differentiation ability and FTO expression under different conditions. (a–c) ALP staining at 3 and 7 days showed decreased ALP activity, and ARS staining at day 14 indicated reduced mineralized nodules in the OM+LPS group compared to the OM group. (d) qRT-PCR revealed elevated mRNA levels of DSPP, DMP1, and COL1 in the OM group while showing decreased levels in the OM+LPS group. (e,f) Western blotting showed increased protein expression of DSPP, DMP1, and COL1 in the OM group, contrasting with decreased expression in the OM+LPS group. (g–i) qRT-PCR and Western blotting showed elevated FTO expression in OM, with reduced expression observed in OM+LPS. Control, growth medium (GM); LPS, GM with 1 µg/mL LPS; OM, odontoblastic induction medium; OM+LPS, OM with 1 µg/mL LPS; hSCAPs, human stem cells from the apical papilla; FTO, fat mass and obesity-associated protein; LPS, lipopolysaccharide; ALP, alkaline phosphatase; ARS, alizarin red S; qRT-PCR, Quantitative Real-Time Polymerase Chain Reaction; DSPP, dentin sialophosphoprotein; DMP1, dentin matrix protein-1; COL1, collagen I.

4). NLRC4-mediated pyroptosis was involved in coagulation disorders of acute pancreatitis. The journal of gene medicine, 2024 (PubMed: 38571451) [IF=3.5]

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FANCG Antibody - #AF0006